scholarly journals Caffeic Acid Phenethyl Ester Inhibits Oral Cancer Cell Metastasis by Regulating Matrix Metalloproteinase-2 and the Mitogen-Activated Protein Kinase Pathway

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Chih-Yu Peng ◽  
Hui-Wen Yang ◽  
Yin-Hung Chu ◽  
Yu-Chao Chang ◽  
Ming-Ju Hsieh ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Matrix Metalloproteinase ◽  
Caffeic Acid ◽  
Cancer Cell ◽  
Cancer Metastasis ◽  
Caffeic Acid Phenethyl Ester ◽  
Matrix Metalloproteinase 2 ◽  
Migration And Invasion ◽  
Oral Cancer Cell ◽  
Cell Metastasis

Caffeic acid phenethyl ester (CAPE), an active component extracted from honeybee hives, exhibits anti-inflammatory and anticancer activities. However, the molecular mechanism by which CAPE affects oral cancer cell metastasis has yet to be elucidated. In this study, we investigated the potential mechanisms underlying the effects of CAPE on the invasive ability of SCC-9 oral cancer cells. Results showed that CAPE attenuated SCC-9 cell migration and invasion at noncytotoxic concentrations (0 μM to 40 μM). Western blot and gelatin zymography analysis findings further indicated that CAPE downregulated matrix metalloproteinase-2 (MMP-2) protein expression and inhibited its enzymatic activity. CAPE exerted its inhibitory effects on MMP-2 expression and activity by upregulating tissue inhibitor of metalloproteinase-2 (TIMP-2) and potently decreased migration by reducing focal adhesion kinase (FAK) phosphorylation and the activation of its downstream signaling molecules p38/MAPK and JNK. These data indicate that CAPE could potentially be used as a chemoagent to prevent oral cancer metastasis.

scholarly journals Pinostilbene Hydrate Suppresses Human Oral Cancer Cell Metastasis by Downregulation of Matrix Metalloproteinase-2 Through the Mitogen-Activated Protein Kinase Signaling Pathway

2018 ◽  
Vol 50 (3) ◽  
pp. 911-923 ◽  
Author(s):  
Ming-Ju Hsieh ◽  
Mei-Chung Chin ◽  
Chia-Chieh Lin ◽  
Yi-Ting His ◽  
Yu-Sheng Lo ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Mitogen Activated Protein Kinase ◽  
Matrix Metalloproteinase 2 ◽  
Migration And Invasion ◽  
Protein Activity ◽  
Oral Cancer Cell ◽  
Cell Metastasis ◽  
Mitogen Activated Protein

Background/Aims: Cancer is the most common cause of death worldwide with approximately one third of people being diagnosed with cancer in their lifetime. Pinostilbene hydrate (PSH) A methylated derivative of resveratrol Has been reported to possess antioxidative Cardioprotective and anticancer properties. However the antimetastatic effect of pinostilbene in oral squamous cell carcinoma (OSCC) remains unknown. Methods: In this study We investigated the effect of PSH on antimetastatic activity and the relevant signaling pathways underlying mechanisms of SCC-9 SAS and HSC-3 oral cancer cell lines by MTT assay Wound healing Transwell assay Zymography and western blot analysis. Results: Our findings indicated that PSH inhibits migration and invasion ability by reducing the protein activity and expression of matrix metalloproteinases-2 (MMP-2) in all three cell lines. Moreover • The phosphorylation of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinases (p38) had significant inhibitory effects in the presence of PSH in the SCC9 and SAS cell lines. A combination of ERK1/2 and p38 inhibitors with PSH also reduced the migration and activity of MMP-2 in the SCC9 and SAS cell lines. Conclusion: This study demonstrated that PSH suppresses MMP-2 enzymatic activity by downregulating the p38/ERK1/2 pathway and that it might be a promising agent for preventing OSCC cell metastasis.

Geraniin inhibits oral cancer cell migration by suppressing matrix metalloproteinase‐2 activation through the FAK/Src and ERK pathways

2019 ◽  
Vol 34 (10) ◽  
pp. 1085-1093 ◽  
Author(s):  
Chia‐Ming Yeh ◽  
Ming‐Ju Hsieh ◽  
Jia‐Sin Yang ◽  
Shun‐Fa Yang ◽  
Yi‐Ting Chuang ◽  
...  
Keyword(s):  
Cell Migration ◽  
Oral Cancer ◽  
Matrix Metalloproteinase ◽  
Cancer Cell ◽  
Matrix Metalloproteinase 2 ◽  
Cancer Cell Migration ◽  
Oral Cancer Cell

Apoptosis induced by caffeic acid phenethyl ester in human oral cancer cell lines: Involvement of Puma and Bax activation

2017 ◽  
Vol 84 ◽  
pp. 94-99 ◽  
Author(s):  
Hyun-Ju Yu ◽  
Ji-Ae Shin ◽  
In-Hyoung Yang ◽  
Dong-Hoon Won ◽  
Chi Hyun Ahn ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Caffeic Acid ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Caffeic Acid Phenethyl Ester ◽  
Oral Cancer Cell

scholarly journals PROPOLIS OR CAFFEIC ACID PHENETHYL ESTER (CAPE) INHIBITS GROWTH AND VIABILITY IN MULTIPLE ORAL CANCER CELL LINES.

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Whitney Saarem ◽  
Fang Yu Wang ◽  
Elena Farfel
Keyword(s):  
Oral Cancer ◽  
Caffeic Acid ◽  
Cell Lines ◽  
Cancer Cell ◽  
Normal Cell ◽  
Cancer Cell Lines ◽  
Caffeic Acid Phenethyl Ester ◽  
Cellular Viability ◽  
Cell Control ◽  
Oral Cancer Cell

Objective: Propolis is a natural antimicrobial resin from honeybee hives that contains caffeic acid phenethyl ester (CAPE), which has anti-proliferative activity against some human cancers, including colon, liver, lung and breast – although limited evidence has evaluated this potential in oral cancers. Based upon this information, the primary objective of this study was to evaluate the anti-tumor effects of CAPE against multiple well-characterized oral cancer cell lines. Methods: Using well-characterized oral cancer cell lines (SCC15, SCC25 and CAL27), CAPE was administered at 100 ug/mL to assess any effects on cellular viability or growth over three days. A normal, non-cancerous cell line (HGF-1) was also included. Results: The results of this pilot study demonstrated that CAPE administration significantly reduced both viability and proliferation in all three oral cancer cell lines.  Viability was significantly reduced between 30.3% and 35.4% among the oral cancer cell lines (p<0.05), but remained unchanged in the HGF-1 normal cell control (p=0.878). Growth was significantly inhibited between 53.1% and 60.6% among the oral cancer cell lines (p<0.05) but was not affected in the HGF-1 normal cell control (p=0.341). Conclusions: Although the reductions in both cellular viability and proliferation were distinct for each cell line, all exhibited a similar trend and were within a narrowly defined range. These results strongly suggest that CAPE administration had a significant and immediate effect on oral cancer growth and viability and therefore should be considered as the basis for future studies as a potential complementary and alternative therapy for oral cancer. Key words: Caffeic Acid Phenethyl Ester (CAPE), Propolis, Oral cancer, Complementary and alternative medicine.

Caffeic acid phenethyl ester upregulates N-myc downstream regulated gene 1 via ERK pathway to inhibit human oral cancer cell growth in vitro and in vivo

2017 ◽  
Vol 61 (9) ◽  
pp. 1600842 ◽  
Author(s):  
Li-Chuan Chung ◽  
Kun-Chun Chiang ◽  
Tsui-Hsia Feng ◽  
Kang-Shuo Chang ◽  
Sung-Ting Chuang ◽  
...  
Keyword(s):  
Cell Growth ◽  
Oral Cancer ◽  
Caffeic Acid ◽  
Cancer Cell ◽  
Caffeic Acid Phenethyl Ester ◽  
Erk Pathway ◽  
Cancer Cell Growth ◽  
Oral Cancer Cell
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