Characterization of Microsomal Bilirubin and p-Nitrophenol Uridine Diphosphate Glucuronosyltransferase Activities in Human Liver: A Comparison with Rat Liver

Enzyme ◽  
1981 ◽  
Vol 26 (2) ◽  
pp. 93-102 ◽  
Author(s):  
Jean-Louis Mahu ◽  
Anne-Marie Preaux ◽  
Philippe Mavier ◽  
Pierre Berthelot
Keyword(s):  
Rat Liver ◽  
Human Liver ◽  
Uridine Diphosphate ◽  
Uridine Diphosphate Glucuronosyltransferase

The effects of UDP-sugars, UDP and Mg2+on uridine diphosphate glucuronosyltransferase activity in human liver microsomes

Xenobiotica ◽  
2017 ◽  
Vol 48 (9) ◽  
pp. 882-890 ◽  
Author(s):  
Gurinder Walia ◽  
Alexander D. Smith ◽  
Zoe Riches ◽  
Abby C. Collier ◽  
Michael W. H. Coughtrie
Keyword(s):  
Human Liver ◽  
Liver Microsomes ◽  
Human Liver Microsomes ◽  
Uridine Diphosphate ◽  
Uridine Diphosphate Glucuronosyltransferase

scholarly journals Purification and immunochemical characterization of a male-specific rat liver oestrogen sulphotransferase

1993 ◽  
Vol 289 (3) ◽  
pp. 719-725 ◽  
Author(s):  
E B Borthwick ◽  
A Burchell ◽  
M W Coughtrie
Keyword(s):  
Rat Liver ◽  
Human Liver ◽  
Regulatory Mechanism ◽  
Biologically Active ◽  
Male Rats ◽  
Immunochemical Characterization ◽  
Male Specific ◽  
The Brain ◽  
Brain Examination

Sulphation of oestrogens represents an important regulatory mechanism for these biologically active compounds. We have characterized and purified a form of rat liver sulphotransferase (ST), existing as a 32,500 Da monomer, which sulphates oestrogens, and have used this preparation to produce antibodies against oestrogen ST. The enzyme was active against oestrone, oestriol and beta-oestradiol, but not towards androgens. Using the antibody as a probe for immunoblotting, it was determined that the enzyme is expressed solely in male rats, and predominantly in the liver. Of the tissues examined, the only major extrahepatic tissue found to have any oestrogen ST was the brain (although the levels were very low), indicating that there might be a role for the sulphation of oestrogens in the brain. Examination of human liver and platelet cytosols by immunoblotting showed that the antibody recognized two major proteins of 32 and 34 kDa, which were presumed to correspond to the two principal phenol ST isoenzymes present in man.

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