The Effect of Birth and Related Hormonal Events on the Neonatal Development of Uridine Diphosphate Glucuronosyltransferase Activity towards Bilirubin and Other Substrates in Rat Liver

1978 ◽  
Vol 6 (1) ◽  
pp. 175-176
Author(s):  
MARY T. CAMPBELL ◽  
GRAHAM J. WISHART
Keyword(s):  
Rat Liver ◽  
Uridine Diphosphate ◽  
Neonatal Development ◽  
Uridine Diphosphate Glucuronosyltransferase

scholarly journals An improved assay technique for uridine diphosphate glucuronosyltransferase activity towards 5-hydroxytryptamine and some properties of the enzyme

1978 ◽  
Vol 175 (3) ◽  
pp. 1119-1124 ◽  
Author(s):  
J E A Leakey
Keyword(s):  
Rat Liver ◽  
Mouse Liver ◽  
Wistar Rat ◽  
Uridine Diphosphate ◽  
Gunn Rat ◽  
Uridine Diphosphate Glucuronosyltransferase ◽  
Membrane Perturbation ◽  
Udp Glucuronosyltransferase ◽  
Routine Assay ◽  
Assay Technique

A simplified and sensitive procedure for the routine assay of UDP-glucuronosyltransferase activity towards 5-hydroxytryptamine (serotonin) was developed and the reaction product confirmed as the O-glucuronide of this substrate. The assay was used to study some properties of this UDP-glucuronosyltransferase activity. In mouse liver activity was stimulated by membrane-perturbation procedures and by UDP-N-acetylglucosamine. In rat liver it was stimulated by digitonin, but not by diethylnitrosamine. Mouse duodenum, kidney, and lung possessed activity that was less latent than in liver. No activity was found in homogenates of brain. The activity was present in Gunn rat liver, though only one-third of that in Wistar rat liver. Cat liver contained no UDP-glucuronosyltransferase activity towards 5-hydroxytryptamine.

Sign in / Sign up

Export Citation Format

Share Document