Dependence on Glucocorticoids of Development of ‘Latent’ and ‘Activated’ Uridine Diphosphate Glucuronosyltransferase Activity in Organ Cultures of Foetal Rat Liver

1976 ◽  
Vol 4 (6) ◽  
pp. 1069-1071 ◽  
Author(s):  
G. J. WISHART ◽  
M. A. GOHEER ◽  
AGNES M. DONALD ◽  
G. J. DUTTON
Keyword(s):  
Rat Liver ◽  
Uridine Diphosphate ◽  
Organ Cultures ◽  
Uridine Diphosphate Glucuronosyltransferase ◽  
Foetal Rat

scholarly journals Precocious development of uridine diphosphate glucuronosyltransferase activity during organ culture of foetal rat liver in the presence of glucocorticoids

1977 ◽  
Vol 166 (2) ◽  
pp. 249-253 ◽  
Author(s):  
G J Wishart ◽  
M A Goheer ◽  
J E A Leakey ◽  
G J Dutton
Keyword(s):  
Rat Liver ◽  
Defined Medium ◽  
Transferase Activity ◽  
Uridine Diphosphate ◽  
Chemically Defined Medium ◽  
Foetal Rat ◽  
First Time ◽  
Precocious Development ◽  
Stimulation Of

1. Precocious development of mammalian UDP-glucuronosyltransferase (EC 2.4.1.1.7) induced by endogenous compounds of known chemical composition is reported for the first time. 2. This development occurs in cultured explants of foetal rat liver when exposed to corticosteroids possessing a pregn-4′-ene structure and a hydroxy or an oxo group at C-11. 3. Explants from 14-day foetuses cultured for 3 days in a chemically defined medium containing dexamethasone exhibited transferase activities towards o-aminophenol within adult male values. Those liver transferase activities attained in utero by 17 days were still negligible. 4. Evidence from several approaches indicated that the explants required glucocorticoids for expression of the transferase, not for maintenance of viability. 5. Glucocorticoid-dependent stimulation of transferase activity required incorporation of L-[14C]leucine into protein, as judged from the pulsing of cultures with cycloheximide. 6. The relevance of these culture experiments to the situation in vivo is discussed.

scholarly journals An improved assay technique for uridine diphosphate glucuronosyltransferase activity towards 5-hydroxytryptamine and some properties of the enzyme

1978 ◽  
Vol 175 (3) ◽  
pp. 1119-1124 ◽  
Author(s):  
J E A Leakey
Keyword(s):  
Rat Liver ◽  
Mouse Liver ◽  
Wistar Rat ◽  
Uridine Diphosphate ◽  
Gunn Rat ◽  
Uridine Diphosphate Glucuronosyltransferase ◽  
Membrane Perturbation ◽  
Udp Glucuronosyltransferase ◽  
Routine Assay ◽  
Assay Technique

A simplified and sensitive procedure for the routine assay of UDP-glucuronosyltransferase activity towards 5-hydroxytryptamine (serotonin) was developed and the reaction product confirmed as the O-glucuronide of this substrate. The assay was used to study some properties of this UDP-glucuronosyltransferase activity. In mouse liver activity was stimulated by membrane-perturbation procedures and by UDP-N-acetylglucosamine. In rat liver it was stimulated by digitonin, but not by diethylnitrosamine. Mouse duodenum, kidney, and lung possessed activity that was less latent than in liver. No activity was found in homogenates of brain. The activity was present in Gunn rat liver, though only one-third of that in Wistar rat liver. Cat liver contained no UDP-glucuronosyltransferase activity towards 5-hydroxytryptamine.

scholarly journals N-Acetylglutamate in rat liver during foetal development

1984 ◽  
Vol 222 (3) ◽  
pp. 837-838 ◽  
Author(s):  
M Van Dijk ◽  
P Lund
Keyword(s):  
Rat Liver ◽  
Urea Cycle ◽  
Developmental Pattern ◽  
Foetal Development ◽  
Tissue Content ◽  
Foetal Rat ◽  
Foetal Life

N-Acetylglutamate is present in foetal rat liver at 17 days' gestation. The tissue content (approx. 50 nmol/g wet wt.) remains constant during later foetal life. The appearance of N-acetylglutamate does not parallel the developmental pattern of the urea cycle.

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