Neutrophil Gelatinase-Associated Lipocalin, but Not Kidney Injury Marker 1, Correlates with Duration of Delayed Graft Function

2015 ◽  
Vol 55 (4) ◽  
pp. 319-327 ◽  
Author(s):  
Eline Kristina van den Akker ◽  
Dennis Alexander Hesselink ◽  
Olivier Christiaan Manintveld ◽  
Jan Nicolaas Maria IJzermans ◽  
Ronald Wilhelm Frederik de Bruijn ◽  
...  
Keyword(s):  
Graft Function ◽  
Kidney Injury ◽  
Delayed Graft Function ◽  
Donor Age ◽  
Serum Samples ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Study Materials ◽  
Early Biomarker ◽  
Neutrophil Gelatinase ◽  
First Time

Background: No specific early biomarker is available to measure kidney injury after kidney transplantation (KT). Both neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury marker 1 (KIM-1) increase after oxidative injury. Their potential as early biomarkers was evaluated in this one-arm pilot study. Materials and Methods: Twenty consecutive KT patients receiving a kidney from a donation after circulatory death donor were included. Graft perfusate was collected, as well as serum samples before transplantation, at the end of surgery, and 1, 4, and 7 days after transplantation. NGAL and KIM-1 were measured using ELISA. Kidney function and delayed graft function (DGF) were monitored. Results: In this cohort, 85% of the KT patients developed DGF. Perfusate NGAL correlated with donor age (r2 = 0.094, p = 0.01) and serum creatinine (r2 = 0.243, p = 0.05). A cardiac cause of death was associated with higher NGAL in the perfusate (p = 0.03). Serum NGAL at day 1 was significantly higher in patients with DGF (730 ng/ml, range 490-1,655, vs. 417 ng/ml, range 232-481; p = 0.01). Serum NGAL levels at day 1, 4, and 7 correlated with the duration of DGF. KIM-1 was not detectable in the perfusate or in the serum until postoperative day 4 in 80% of patients. Conclusions: NGAL in the perfusate correlates with known donor risk factors for DGF. For the first time, we describe that serum NGAL at day 1 can discriminate between DGF and immediate graft function. Also, serum NGAL levels at day 1, 4, and 7 correlate with the duration of DGF. No association with KIM-1 was found. These data suggest that NGAL may be used as an early biomarker to detect DGF and warrants further study.

Neutrophil gelatinase-associated lipocalin, but not kidney injury marker-1, correlates with duration of delayed graft function

2014 ◽  
Vol 31 (4) ◽  
pp. 230
Author(s):  
E.K. van den Akker ◽  
D.A. Hesselink ◽  
O.C. Manintveld ◽  
J.N.M. IJzermans ◽  
R.W.F. de Bruin ◽  
...  
Keyword(s):  
Graft Function ◽  
Kidney Injury ◽  
Delayed Graft Function ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Neutrophil Gelatinase

scholarly journals Alkalmas-e a vizelet neutrofil gelatináz asszociálta lipokalin meghatározása a rejekció előrejelzésére veseátültetés után?

2015 ◽  
Vol 156 (48) ◽  
pp. 1956-1959
Author(s):  
Gábor Telkes ◽  
Gábor Dallos ◽  
Marina Varga
Keyword(s):  
Negative Impact ◽  
Graft Function ◽  
Kidney Injury ◽  
Delayed Graft Function ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Kidney Recipients ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Neutrophil Gelatinase

Introduction: Delayed graft function and acute rejection have negative impact on graft survival. Aim: To asses the predictive value of urinary neutrophil gelatinase-associated lipocalin, which has been found to be a promising biomarker for the diagnosis of acute kidney injury. Method: In this prospective study urinary neutrophil gelatinase-associated lipocalin levels of 27 kidney recipients were measured. Results: Patients were grouped as follows: group 1, no complication; group 2, rejection; group 3, delayed graft function requiring dialysis; group 4, rejection plus delayed graft function. There were no significant differences between groups 1 and 2, and between groups 3 and 4. Patients in groups 3 and 4 had significantly higher urinary neutrophil gelatinase-associated lipocalin levels as compared to those in groups 3 and 4. There was a paralIel change in urinary neutrophil gelatinase-associated lipocalin levels in groups 1 and 2. Conclusions: In these patients urinary neutrophil gelatinase-associated lipocalin levels failed to provide useful information in both cases of normal and impaired function. Orv. Hetil., 2015, 156(48), 1956–1959.

Urinary neutrophil gelatinase-associated lipocalin as an early biomarker for acute kidney injury in dogs

2020 ◽  
Vol 255 ◽  
pp. 105423
Author(s):  
S. Scheemaeker ◽  
E. Meyer ◽  
J.P. Schoeman ◽  
P. Defauw ◽  
L. Duchateau ◽  
...  
Keyword(s):  
Acute Kidney Injury ◽  
Kidney Injury ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Early Biomarker ◽  
Neutrophil Gelatinase

Assessment of Neutrophil Gelatinase-associated Lipocalin in the Brain-dead Organ Donor to Predict Immediate Graft Function in Kidney Recipients

2015 ◽  
Vol 122 (1) ◽  
pp. 96-105 ◽  
Author(s):  
Laurent Muller ◽  
Armelle Nicolas-Robin ◽  
Sophie Bastide ◽  
Orianne Martinez ◽  
Guillaume Louart ◽  
...  
Keyword(s):  
Renal Replacement Therapy ◽  
Replacement Therapy ◽  
Odds Ratio ◽  
Graft Function ◽  
Delayed Graft Function ◽  
Receiver Operating Curve ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Kidney Recipients ◽  
Brain Dead ◽  
Neutrophil Gelatinase

Abstract Background: Delayed graft function is a major determinant of long-term renal allograft survival. Despite considerable efforts to improve donor selection and matching, incidence of delayed graft function remains close to 25%. As neutrophil gelatinase-associated lipocalin (NGAL) has been shown to predict acute renal failure, the authors tested the hypothesis that NGAL measurement in brain-dead donors predicts delayed graft function in kidney recipients. Methods: In a prospective, multicenter, observational study, serum NGAL was measured in donors at the time of transfer to operating room. The primary endpoint was the delayed graft function, defined as the need for renal replacement therapy during the first week posttransplantation. Results: Among 159 included brain-dead donors, 146 were analyzable leading to 243 renal transplantations. Of these, 56 (23%) needed renal replacement therapy. Donors’ NGAL values were similar in case of both delayed and normal graft function in recipients. The area under the receiver-operating curve for NGAL to predict the need for renal replacement therapy before day 8 was 0.50 (95% CI, 0.42 to 0.59). The area under curve for NGAL to predict failure to return to a normal graft function at day 8 was 0.51 (95% CI, 0.44 to 0.59). Using multivariate analysis, NGAL was not associated to the need for renal replacement therapy (odds ratio, 0.99; 95% CI, 0.98 to1.00) or failure to return to a normal graft function at day 8 (odds ratio, 1.00; 95% CI, 0.99 to 1.00). Conclusion: NGAL measurement in brain-dead donors at the time of recovery failed to predict delayed or normal graft function in kidney recipients.

scholarly journals Dual Actions of A2A and A3 Adenosine Receptor Ligand Prevents Obstruction-Induced Kidney Fibrosis in Mice

2021 ◽  
Vol 22 (11) ◽  
pp. 5667
Author(s):  
Eun Seon Pak ◽  
Lak Shin Jeong ◽  
Xiyan Hou ◽  
Sushil K. Tripathi ◽  
Jiyoun Lee ◽  
...  
Keyword(s):  
Kidney Injury ◽  
Tubulointerstitial Fibrosis ◽  
Cellular Damage ◽  
Kidney Fibrosis ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
A3 Adenosine Receptor ◽  
Kidney Morphology ◽  
Neutrophil Gelatinase ◽  
First Time ◽  
And Oxidative Stress

Kidney fibrosis is the final outcome of chronic kidney disease (CKD). Adenosine plays a significant role in protection against cellular damage by activating four subtypes of adenosine receptors (ARs), A1AR, A2AAR, A2BAR, and A3AR. A2AAR agonists protect against inflammation, and A3AR antagonists effectively inhibit the formation of fibrosis. Here, we showed for the first time that LJ-4459, a newly synthesized dual-acting ligand that is an A2AAR agonist and an A3AR antagonist, prevents the progression of tubulointerstitial fibrosis. Unilateral ureteral obstruction (UUO) surgery was performed on 6-week-old male C57BL/6 mice. LJ-4459 (1 and 10 mg/kg) was orally administered for 7 days, started at 1 day before UUO surgery. Pretreatment with LJ-4459 improved kidney morphology and prevented the progression of tubular injury as shown by decreases in urinary kidney injury molecular-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL) excretion. Obstruction-induced tubulointerstitial fibrosis was attenuated by LJ-4459, as shown by a decrease in fibrotic protein expression in the kidney. LJ-4459 also inhibited inflammation and oxidative stress in the obstructed kidney, with reduced macrophage infiltration, reduced levels of pro-inflammatory cytokines, as well as reduced levels of reactive oxygen species (ROS). These data demonstrate that LJ-4459 has potential as a therapeutic agent against the progression of tubulointerstitial fibrosis.

scholarly journals Neutrophil gelatinase‐associated lipocalin: An early biomarker for predicting acute kidney injury and severity in patients with acute pancreatitis

JGH Open ◽  
2018 ◽  
Vol 3 (2) ◽  
pp. 105-110 ◽  
Author(s):  
Pradeep K Siddappa ◽  
Rakesh Kochhar ◽  
Pooja Sarotra ◽  
Bikas Medhi ◽  
Vivekanand Jha ◽  
...  
Keyword(s):  
Acute Kidney Injury ◽  
Acute Pancreatitis ◽  
Kidney Injury ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Early Biomarker ◽  
Neutrophil Gelatinase
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