Investigations on the Preservation of Blood by Rapid Freezing and Thawing Procedures

2015 ◽  
pp. 439-442 ◽  
Author(s):  
A. P. Rinfret ◽  
G. F. Doebbler ◽  
C. W. Cowley
Keyword(s):  
Rapid Freezing ◽  
Freezing And Thawing

Viability of ovarian tissue after freezing

1957 ◽  
Vol 147 (929) ◽  
pp. 520-528 ◽  
Keyword(s):  
Systematic Study ◽  
Normal Saline ◽  
Ovarian Tissue ◽  
Cumulus Cells ◽  
Rapid Freezing ◽  
Freezing And Thawing ◽  
Slow Cooling ◽  
Rapid Cooling ◽  
Fertilized Egg ◽  
Cells Cultured

The fertilized egg of the rabbit, obtained from the Fallopian tube, is very sensitive to freezing and thawing, even after treatment with glycerol (Smith 1953). By contrast, the cumulus cells of the follicular granulosa which adhere to the egg after ovulation are much more resistant, and a few survive without any special precautions being taken (Smith 1949). A systematic study of the viability of the cumulus cells cultured after freezing and thawing by various methods (Smith 1953) gave the following results: (1) A majority of the cells survived when suspended in homologous serum, cooled slowly to — 79° C and thawed rapidly at + 40° C. Very few survived rapid freezing. (2) Addition of 15 % glycerol to the suspending medium improved survival after slow cooling but not after rapid cooling. (3) Cells suspended in normal saline failed to survive either slow or rapid cooling. Addition of glycerol promoted some survival after slow cooling. These experiments emphasized the need for slow cooling, already demonstrated with bull spermatozoa, and the superiority of serum over saline as a suspending medium. The addition of glycerol to the medium, though conducive to survival after freezing and thawing, was not as necessary as with spermatozoa.

In vivo survival of platelet concentrates following rapid freezing and thawing

Cryobiology ◽  
1969 ◽  
Vol 5 (6) ◽  
pp. 379-384 ◽  
Author(s):  
H. Pfisterer ◽  
F. Weber ◽  
G. Michlmayr
Keyword(s):  
Rapid Freezing ◽  
Platelet Concentrates ◽  
Freezing And Thawing

Rapid freezing of the mouse blastocyst: effects of cryoprotectants and of time and temperature of exposure to cryoprotectant before direct plunging into liquid nitrogen

1991 ◽  
Vol 3 (2) ◽  
pp. 175 ◽  
Author(s):  
R Li ◽  
A Trounson
Keyword(s):  
Liquid Nitrogen ◽  
Room Temperature ◽  
Rapid Freezing ◽  
High Concentration ◽  
Freezing And Thawing ◽  
Initial Exposure ◽  
Survival And Development ◽  
High Concentrations

This study investigates the effects of time and temperature of exposure to a high concentration (4.5 M) of dimethyl sulfoxide (DMSO), glycerol, 1,2-propanediol (PROH), or a mixture of DMSO and glycerol (DG) in a solution containing 0.25 M sucrose, on the survival and development of rapidly frozen mouse blastocysts. Embryos had significantly (P less than 0.01) higher rates of survival and development when exposed to cryoprotectant at 0 degree C compared with room temperature. The time of exposure to cryoprotectant at either 0 degree C or room temperature before being plunged into liquid nitrogen significantly (P less than 0.01) affected the survival and development of frozen-thawed embryos. Survival and development of blastocysts in vitro and in vivo was significantly (P less than 0.05) higher when exposed at 0 degree C for 10 min to DG, DMSO and glycerol than to PROH. It is concluded that, unlike early-cleavage stage embryos, blastocysts need to be equilibrated at a low temperature (0 degree C) with high concentrations of cryoprotectant before rapid freezing. Exposure of blastocysts to 4.5 M cryoprotectant and 0.25 M sucrose at room temperature either was toxic or else markedly reduced their viability after freezing and thawing, depending on the duration of the initial exposure.

scholarly journals A novel decellularization method to produce brain scaffolds

2019 ◽  
Author(s):  
Alessandro E.C. Granato ◽  
Edgar Ferreira da Cruz ◽  
Dorival Mendes Rodrigues-Junior ◽  
Amanda Cristina Mosini ◽  
Henning Ulrich ◽  
...  
Keyword(s):  
Cell Survival ◽  
Low Cost ◽  
Sodium Dodecyl ◽  
Rapid Freezing ◽  
Neural Cells ◽  
3D Scaffold ◽  
Freezing And Thawing ◽  
Simple Method ◽  
Cell Growth And Differentiation ◽  
First Time

ABSTRACTScaffolds composed of extracellular matrix (ECM) can assist tissue remodeling and repair following injury. The ECM is a complex biomaterial composed of proteins, glycoproteins, proteoglycans, and glycosaminoglycans, secreted by cells. The ECM contains fundamental biological cues that modulate cell behavior and serves as a structural scaffold for cell adhesion and growth. For clinical applications, where immune rejection is a constraint, ECM can be processed using decellularization methods intended to remove cells and donor antigens from tissue or organs, while preserving native biological cues essential for cell growth and differentiation. Recent studies show bioengineered organs composed by a combination of a diversity of materials and stem cells as a possibility of new therapeutic strategies to treat diseases that affect different tissues and organs, including the central nervous system (CNS). Nevertheless, the methodologies currently described for brain decellularization involve the use of several chemical reagents with many steps that ultimately limit the process of organ or tissue recellularization. Here, we describe for the first time a fast and straightforward method for complete decellularization of mice brain by the combination of rapid freezing and thawing following the use of only one detergent (Sodium dodecyl sulfate (SDS)). Our data show that using the protocol we describe here the brain can be entirely decellularized, while still maintaining ECM components that are essential for cell survival and repopulation of the scaffold. Our results also show the repopulation of the decellularized brain matrix with Neuro2a cells, that were identified by immunohistochemistry in their undifferentiated form. We conclude that this novel and simple method for brain decellularization can be used as a biocompatible scaffold for cell repopulation.Impact StatementFor the first time we describe an easy, effective and low cost method for complete decellularization of murine brain by the use of only one detergent (SDS) combined with rapid freezing and thawing, that can be used as a 3D scaffold for cell culture of neuronal cells. The results show that the decellularized brains still maintain ECM components essential for cell survival and repopulation of the scaffold. Moreover, we found that the decellularized brain matrix can be repopulated with neural cells, showing its biocompatibility.GRAFICAL ABSTRACT

scholarly journals Motility of Bull Spermatozoa after Rapid-freezing and Thawing

1979 ◽  
Vol 50 (10) ◽  
pp. 683-688
Author(s):  
Hajime MIYAMOTO ◽  
Yoshimasa NISHIKAWA
Keyword(s):  
Rapid Freezing ◽  
Freezing And Thawing

scholarly journals Effect of freezing and thawing rates on sperm motility in Bocachico Prochilodus magdalenae (Pisces, Characiformes)

2013 ◽  
pp. 3295-3303 ◽  
Author(s):  
José G. Martínez ◽  
Sandra Pardo C
Keyword(s):  
Slow Freezing ◽  
Rapid Freezing ◽  
Sperm Viability ◽  
Sperm Cryopreservation ◽  
Freezing And Thawing ◽  
Straight Line ◽  
Independent Effects ◽  
Thawing Rate ◽  
Main Effects ◽  
Freezing And Thawing Rates

ABSTRACTObjective. To determine the freezing and thawing rates necessary to maintain sperm viability during cryopreservation of Bocachico semen. Materials and methods. Four interactional treatments were implemented between two freezing (rapid and slow) and two thawing (rapid and slow) curves, in a 2x2 factorial as follows: rapid freezing-rapid thawing, rapid freezing-slow thawing, slow freezing-rapid thawing, and slow freezing-slow thawing. After thawing by Sperm Class Analyzer (SCA) curvilinear velocity (VCL) and straight-line (VSL) (μm sec-1) were analyzed; total, rapid, medium, and slow motility, were compared among treatments. Results. The rapid freezing-slow thawing treatment was lethal for all variables of velocity and motility, causing a significant (p<0.01) post-thaw inmotility of 100%. The slow freezing-rapid thawing interaction had a significantly higher effect than the other treatments (p<0.05), particularly on variables such as rapid motility (10.1 ± 1.1%), medium motility (30.16 ± 4.1%), and curvilinear velocity (51.5 ± 4.75 μm sec.-1) also decreased the percentage of sperm with slow motility (41.7 ± 4.45%). Independently of the applied thawing rate, the freezing rate generated the main significant effect on total motility. Conclusions. It is possible to conclude that the interaction effect between freezing and thawing rates is nil (except for slow motility) during cryopreservation process. However, the independent effects of these factors (main effects) on remaining motility variables are positively significant and decisive to the maintenance of these features, especially the freeze factor (when it is slow). This becomes the first successful report of sperm cryopreservation from Bocachico Prochilodus magdalenae in the world and may be used in conservation programs for this endangered species.

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