scholarly journals Effect of freezing and thawing rates on sperm motility in Bocachico Prochilodus magdalenae (Pisces, Characiformes)

2013 ◽  
pp. 3295-3303 ◽  
Author(s):  
José G. Martínez ◽  
Sandra Pardo C
Keyword(s):  
Slow Freezing ◽  
Rapid Freezing ◽  
Sperm Viability ◽  
Sperm Cryopreservation ◽  
Freezing And Thawing ◽  
Straight Line ◽  
Independent Effects ◽  
Thawing Rate ◽  
Main Effects ◽  
Freezing And Thawing Rates

ABSTRACTObjective. To determine the freezing and thawing rates necessary to maintain sperm viability during cryopreservation of Bocachico semen. Materials and methods. Four interactional treatments were implemented between two freezing (rapid and slow) and two thawing (rapid and slow) curves, in a 2x2 factorial as follows: rapid freezing-rapid thawing, rapid freezing-slow thawing, slow freezing-rapid thawing, and slow freezing-slow thawing. After thawing by Sperm Class Analyzer (SCA) curvilinear velocity (VCL) and straight-line (VSL) (μm sec-1) were analyzed; total, rapid, medium, and slow motility, were compared among treatments. Results. The rapid freezing-slow thawing treatment was lethal for all variables of velocity and motility, causing a significant (p<0.01) post-thaw inmotility of 100%. The slow freezing-rapid thawing interaction had a significantly higher effect than the other treatments (p<0.05), particularly on variables such as rapid motility (10.1 ± 1.1%), medium motility (30.16 ± 4.1%), and curvilinear velocity (51.5 ± 4.75 μm sec.-1) also decreased the percentage of sperm with slow motility (41.7 ± 4.45%). Independently of the applied thawing rate, the freezing rate generated the main significant effect on total motility. Conclusions. It is possible to conclude that the interaction effect between freezing and thawing rates is nil (except for slow motility) during cryopreservation process. However, the independent effects of these factors (main effects) on remaining motility variables are positively significant and decisive to the maintenance of these features, especially the freeze factor (when it is slow). This becomes the first successful report of sperm cryopreservation from Bocachico Prochilodus magdalenae in the world and may be used in conservation programs for this endangered species.

Rates and intensity of freeze–thaw cycles affect nitrous oxide and carbon dioxide emissions from agricultural soils

2019 ◽  
Vol 99 (4) ◽  
pp. 472-484 ◽  
Author(s):  
David E. Pelster ◽  
Martin H. Chantigny ◽  
Philippe Rochette ◽  
Normand Bertrand ◽  
Denis A. Angers ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Nitrous Oxide ◽  
Carbon Dioxide Emissions ◽  
Slow Freezing ◽  
Control Treatment ◽  
Rapid Freezing ◽  
Silty Clay ◽  
Freezing And Thawing ◽  
Freeze Thaw ◽  
Freezing And Thawing Rates

In cool temperate regions, large emissions of nitrous oxide (N2O), an important greenhouse and ozone-depleting gas, have been observed during freeze–thaw (FT) cycles. However, it is unclear how freezing and thawing rates, freezing intensity, and freezing duration influence N2O emissions. We used a laboratory incubation to measure N2O emissions from two soils (sandy loam, silty clay) undergoing a single FT cycle of various freezing and thawing rates [rapid (0.5 °C h−1) vs. slow (0.017 °C h−1)], freezing intensity (−1 vs. −3 °C), and freezing duration (24 vs. 48 freezing degree-days). In general, soil carbon dioxide fluxes during freezing were highest when soils were frozen slowly at −1 °C, whereas fluxes after thawing were highest from the soils frozen and thawed rapidly at −3 °C. Soil N2O emissions during both the freezing and thawing periods were greatest in the soils exposed to rapid freezing to −3 °C, intermediate under rapid freezing to −1 °C and slow freezing to −3 °C, and smallest under slow freezing to −1 °C and the control treatment (constant +1 °C). The similar N2O emissions between the unfrozen control and the slowly frozen −1 °C treatment was unexpected as previous field studies with similar freezing rates and temperatures still experienced high N2O emissions during thaw. This suggests that the physical disruptions caused by freezing and thawing of the surface soil are not the primary driver of FT-induced N2O emissions under field conditions.

GIANT PANDA SPERM TOLERATE CRYOPRESERVATION AT RAPID FREEZING AND THAWING RATES

2007 ◽  
Vol 77 (Suppl_1) ◽  
pp. 107-108 ◽  
Author(s):  
Copper Aitken-Palmer ◽  
Rong Hou ◽  
David Wildt ◽  
Mary Ann Ottinger ◽  
Rebecca Spindler ◽  
...  
Keyword(s):  
Giant Panda ◽  
Rapid Freezing ◽  
Freezing And Thawing ◽  
Freezing And Thawing Rates

scholarly journals Comparative analysis between slow freezing and ultra-rapid freezing for human sperm cryopreservation

2018 ◽  
Author(s):  
Natalí S. Riva ◽  
Claudio Ruhlmann ◽  
Rocío S. Iaizzo ◽  
Carla A. Marcial López ◽  
Alejandro Gustavo Martínez
Keyword(s):  
Comparative Analysis ◽  
Human Sperm ◽  
Slow Freezing ◽  
Rapid Freezing ◽  
Sperm Cryopreservation

scholarly journals Sperm Cryopreservation in American Flamingo (Phoenicopterus Ruber): Influence of Cryoprotectants and Seminal Plasma Removal

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 203
Author(s):  
María Gemma Millán de la Blanca ◽  
Eva Martínez-Nevado ◽  
Cristina Castaño ◽  
Juncal García ◽  
Berenice Bernal ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Genetic Material ◽  
First Year ◽  
Sperm Cryopreservation ◽  
Straight Line ◽  
The Family ◽  
Second Year ◽  
American Flamingo ◽  
Phoenicopterus Ruber

The American flamingo is a useful model for the development of successful semen cryopreservation procedures to be applied to threatened related species from the family Phoenicopteridae, and to permit genetic material banking. Current study sought to develop effective sperm cryopreservation protocols through examining the influences of two permeating cryoprotectants and the seminal plasma removal. During two consecutive years (April), semen samples were collected and frozen from American flamingos. In the first year, the effect of two permeating cryoprotectants, DMA (dimethylacetamide) (6%) or Me2SO (dimethylsulphoxide) (8%), on frozen–thawed sperm variables were compared in 21 males. No differences were seen between DMA and Me2SO for sperm motility, sperm viability, and DNA fragmentation after thawing. In the second year, the role of seminal plasma on sperm cryoresistance was investigated in 31 flamingos. Sperm samples were cryopreserved with and without seminal plasma, using Me2SO (8%) as a cryoprotectant. The results showed that samples with seminal plasma had higher values than samples without seminal plasma for the following sperm variables: Straight line velocity (22.40 µm/s vs. 16.64 µm/s), wobble (75.83% vs. 69.40%), (p < 0.05), linearity (62.73% vs. 52.01%) and straightness (82.38% vs. 73.79%) (p < 0.01); but acrosome integrity was lower (55.56% vs. 66.88%) (p < 0.05). The cryoresistance ratio (CR) was greater in samples frozen with seminal plasma than without seminal plasma for CR-progressive motility (138.72 vs. 54.59), CR-curvilinear velocity (105.98 vs. 89.32), CR-straight line velocity (152.77 vs. 112.58), CR-average path velocity (122.48 vs. 98.12), CR-wobble (111.75 vs. 102.04) (p < 0.05), CR-linearity (139.41 vs. 113.18), and CR-straightness (124.02 vs. 109.97) (p < 0.01). This research demonstrated that there were not differences between Me2SO and DMA to successful freezing sperm of flamingos; seminal plasma removal did not provide a benefit for sperm cryopreservation.

Viability of ovarian tissue after freezing

1957 ◽  
Vol 147 (929) ◽  
pp. 520-528 ◽  
Keyword(s):  
Systematic Study ◽  
Normal Saline ◽  
Ovarian Tissue ◽  
Cumulus Cells ◽  
Rapid Freezing ◽  
Freezing And Thawing ◽  
Slow Cooling ◽  
Rapid Cooling ◽  
Fertilized Egg ◽  
Cells Cultured

The fertilized egg of the rabbit, obtained from the Fallopian tube, is very sensitive to freezing and thawing, even after treatment with glycerol (Smith 1953). By contrast, the cumulus cells of the follicular granulosa which adhere to the egg after ovulation are much more resistant, and a few survive without any special precautions being taken (Smith 1949). A systematic study of the viability of the cumulus cells cultured after freezing and thawing by various methods (Smith 1953) gave the following results: (1) A majority of the cells survived when suspended in homologous serum, cooled slowly to — 79° C and thawed rapidly at + 40° C. Very few survived rapid freezing. (2) Addition of 15 % glycerol to the suspending medium improved survival after slow cooling but not after rapid cooling. (3) Cells suspended in normal saline failed to survive either slow or rapid cooling. Addition of glycerol promoted some survival after slow cooling. These experiments emphasized the need for slow cooling, already demonstrated with bull spermatozoa, and the superiority of serum over saline as a suspending medium. The addition of glycerol to the medium, though conducive to survival after freezing and thawing, was not as necessary as with spermatozoa.

scholarly journals Cryopreservation: The secret of modern preservation of brewer’s yeasts – minireview

2021 ◽  
Vol 67 (1) ◽  
pp. 403-408
Author(s):  
Katarína Hanzalíková ◽  
Petra Kubizniakova ◽  
Lucie Kyselová ◽  
Dagmar Matoulková
Keyword(s):  
Cell Damage ◽  
Cellular Structures ◽  
Biological Functions ◽  
Freezing And Thawing ◽  
Thawing Processes ◽  
Thawing Rate ◽  
Brewer’S Yeasts ◽  
Long Term Preservation ◽  
Stress Accumulation

The aim of the long-term preservation of cells, tissues and organs is to maintain their cellular structures and biological functions for as long as possible. Cryopreservation is a process where biological material is stored and preserved at very low temperatures. However, freezing and thawing processes can cause irreversible cell damage, which is related to formation of ice crystals, osmotic stress, accumulation of reactive forms of oxygen, etc. Therefore the cell viability depends mainly on the freezing rate, the composition of the cryoprotective medium as well as on the thawing rate. Using a suitable cryoprotective medium can increase the viability rate of the yeasts after “revitalization“. Appropriate pre-cultivation before freezing also plays an important role. These facts show that cell freezing and thawing processes must be controlled to avoid cell damage.

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