The Histochemical Demonstration of Aluminum within Tissue Sections

2015 ◽  
pp. 120-123 ◽  
Author(s):  
A. J. Freemont
Keyword(s):  
Histochemical Demonstration ◽  
Tissue Sections

Histochemical Demonstration of Copper and Copper-Associated Protein in the Canine Liver

1985 ◽  
Vol 22 (4) ◽  
pp. 327-332 ◽  
Author(s):  
L. P. Thornburg ◽  
M. Beissenherz ◽  
M. Dolan ◽  
M. F. Raisbeck
Keyword(s):  
Atomic Absorption ◽  
Histochemical Demonstration ◽  
Atomic Absorption Analysis ◽  
Absorption Analysis ◽  
Tissue Sections ◽  
Histochemical Methods ◽  
Histochemical Detection ◽  
Rubeanic Acid ◽  
Copper Detection

Three different histochemical methods for copper detection were compared. Atomic absorption analysis was used to substantiate the tissue stains. There was good correlation between rhodanine staining and rubeanic acid-stained tissue sections. The orcein reaction for copper-associated protein did not consistently correlate with the methods demonstrating copper. Prolonged staining (72 hours) with rubeanic acid more consistently and clearly detected increased copper in canine livers than did staining with rhodanine. Seventy-two hour staining with rubeanic acid is the method of choice for histochemical detection of copper in canine liver.

Histochemical demonstration of hydrogen peroxide production by leukocytes in fixed-frozen tissue sections of inflammatory lesions

1994 ◽  
Vol 56 (4) ◽  
pp. 436-443 ◽  
Author(s):  
Arthur M. Dannenberg ◽  
Brian H. Schofield ◽  
Jay B. Rao ◽  
Theresa T. Dinh ◽  
Ki Lee ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Histochemical Demonstration ◽  
Hydrogen Peroxide Production ◽  
Inflammatory Lesions ◽  
Tissue Sections ◽  
Frozen Tissue

scholarly journals MICROSCOPIC HISTOCHEMICAL DEMONSTRATION OF STEROID-3β-OL DEHYDROGENASE IN TISSUE SECTIONS

1958 ◽  
Vol 6 (4) ◽  
pp. 225-232 ◽  
Author(s):  
LEE W. WATTENBERG
Keyword(s):  
Corpus Luteum ◽  
Adrenal Cortex ◽  
Positive Reaction ◽  
Interstitial Cells ◽  
Histochemical Demonstration ◽  
Biochemical Data ◽  
Tissue Sections

A procedure for the microscopic histochemical demonstration of steroid-3β-ol dehydrogenase has been described. In agreement with reported quantitative biochemical data, positive reactions have been obtained in the adrenal cortex, corpus luteum, placenta, and interstitial cells of the testis. In addition, a strongly positive reaction is obtained in the interstitial cells of the rodent ovary.

Histochemical Demonstration of Enzyme Activities in Plastic and Paraffin Embedded Tissue Sections

1979 ◽  
Vol 54 (1) ◽  
pp. 5-12 ◽  
Author(s):  
Sadanobu Higuchi ◽  
Moritaka Suga ◽  
Arthur M. Dannenberg ◽  
Brian H. Schofield
Keyword(s):  
Enzyme Activities ◽  
Histochemical Demonstration ◽  
Tissue Sections

scholarly journals Microspectrophotometric quantitation of the diaminobenzidine reaction for histochemical demonstration of cytochrome oxidase activity.

1978 ◽  
Vol 26 (3) ◽  
pp. 157-162 ◽  
Author(s):  
A C Frasch ◽  
M E Itoiz ◽  
R L Cabrini
Keyword(s):  
Cytochrome Oxidase ◽  
Incubation Medium ◽  
Histochemical Demonstration ◽  
Tongue Muscle ◽  
Tissue Sections ◽  
Direct Light ◽  
Fixed Concentration ◽  
Set Up ◽  
Cryostat Sections

Polyacrylamide models in which an extract of cattle heart mitochondria was incorporated, as well as cryostat sections of tongue muscle and epithelium, were used to set up the conditions under which the histochemical reaction for the demonstration of cytochrome oxidase can be quantitated. Using diaminobenzidine in a concentration of 5.5 mM, cytochrome C in a fixed concentration of 76 micron and keeping the incubation medium away from direct light action, enzyme activity can be evaluated by means of direct microphotometry on tissue sections. Each biologic model requires previous individual determination of the measurement limits. These limits can be readily established by using a small chamber for the incubation medium, which can be placed in the microphotometer, allowing the reaction rate to be following using a single section.

scholarly journals HISTOCHEMICAL DEMONSTRATION OF MITOCHONDRIAL ADENOSINE TRIPHOSPHATASE ACTIVITY IN TISSUE SECTIONS

1962 ◽  
Vol 10 (1) ◽  
pp. 65-74 ◽  
Author(s):  
MAX WACHSTEIN ◽  
MAIRE BRADSHAW ◽  
JOSÉ M. ORTIZ
Keyword(s):  
Adenosine Triphosphatase ◽  
Histochemical Demonstration ◽  
The Other ◽  
Mitochondrial Activity ◽  
Bile Canaliculi ◽  
Adenosine Triphosphatase Activity ◽  
Tissue Sections ◽  
Dog Kidney ◽  
Cryostat Sections ◽  
Neutral Formalin

A comparative study was made of the distribution of mitochondrial adenosine triphosphatase activity in several organs of rat and rabbit using both the calcium and lead techniques. Certain modifications in these techniques assured both improved intracellular morphology and considerable preservation of enzyme activity. Of particular importance was the low temperature (–2 to –3°C) of the neutral calcium-formol solution, and, following short fixation (lead method), a subsequent wash in neutral buffer. Mitochondrial activity was similar with both techniques, but the lead method proved to be less costly, less time-consuming, and above all, far less capricious than the calcium technique. In good preparations, the appearance of mitochondria is as clearly defined as in sections stained with non-enzymatic, conventional techniques. Long exposure of cryostat sections to formalin or preparation of sections from tissue blocks fixed in neutral formalin leads to the complete abolition of mitochondrial activity; on the other hand, it accentuates enzyme staining of other structures, such as, for instance, bile canaliculi in the liver and the secretory capillaries in the pancreas and salivary glands. It also visualizes the infolding membranes in certain tubules of the rat and dog kidney. It is assumed that formalin-fixation aids in the enzymato-morphologic distinction of these two different intracellular structures.

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