scholarly journals MICROSCOPIC HISTOCHEMICAL DEMONSTRATION OF STEROID-3β-OL DEHYDROGENASE IN TISSUE SECTIONS

1958 ◽  
Vol 6 (4) ◽  
pp. 225-232 ◽  
Author(s):  
LEE W. WATTENBERG
Keyword(s):  
Corpus Luteum ◽  
Adrenal Cortex ◽  
Positive Reaction ◽  
Interstitial Cells ◽  
Histochemical Demonstration ◽  
Biochemical Data ◽  
Tissue Sections

A procedure for the microscopic histochemical demonstration of steroid-3β-ol dehydrogenase has been described. In agreement with reported quantitative biochemical data, positive reactions have been obtained in the adrenal cortex, corpus luteum, placenta, and interstitial cells of the testis. In addition, a strongly positive reaction is obtained in the interstitial cells of the rodent ovary.

Histochemical Demonstration of Copper and Copper-Associated Protein in the Canine Liver

1985 ◽  
Vol 22 (4) ◽  
pp. 327-332 ◽  
Author(s):  
L. P. Thornburg ◽  
M. Beissenherz ◽  
M. Dolan ◽  
M. F. Raisbeck
Keyword(s):  
Atomic Absorption ◽  
Histochemical Demonstration ◽  
Atomic Absorption Analysis ◽  
Absorption Analysis ◽  
Tissue Sections ◽  
Histochemical Methods ◽  
Histochemical Detection ◽  
Rubeanic Acid ◽  
Copper Detection

Three different histochemical methods for copper detection were compared. Atomic absorption analysis was used to substantiate the tissue stains. There was good correlation between rhodanine staining and rubeanic acid-stained tissue sections. The orcein reaction for copper-associated protein did not consistently correlate with the methods demonstrating copper. Prolonged staining (72 hours) with rubeanic acid more consistently and clearly detected increased copper in canine livers than did staining with rhodanine. Seventy-two hour staining with rubeanic acid is the method of choice for histochemical detection of copper in canine liver.

scholarly journals Targeted disruption of the aromatase P450 gene (Cyp19) in mice and their ovarian and uterine responses to 17beta-oestradiol

2001 ◽  
Vol 170 (1) ◽  
pp. 99-111 ◽  
Author(s):  
K Toda ◽  
K Takeda ◽  
T Okada ◽  
S Akira ◽  
T Saibara ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Corpus Luteum ◽  
Lipid Droplets ◽  
Smooth Endoplasmic Reticulum ◽  
Ovarian Follicles ◽  
Interstitial Cells ◽  
Aromatase Activity ◽  
Tubular Structures ◽  
Wild Type ◽  
Targeted Disruption

Aromatase P450 (CYP19) is an enzyme catalysing the conversion of androgens into oestrogens. We generated mice lacking aromatase activity (ArKO) by targeted disruption of Cyp19 and report the characteristic features of the ArKO ovaries and uteri as revealed by histological and biochemical analyses. ArKO females were totally infertile but there were as many developing follicles in their ovaries at 8 weeks of age as in wild-type ovaries. Nevertheless, no typical corpus luteum was observed in the ArKO ovaries. Electron microscopy revealed the presence of well-developed smooth endoplasmic reticulum, few lipid droplets and mitochondria with less organized tubular structures in the ArKO luteinized interstitial cells. These ultrastructural features were different from those of the wild-type interstitial cells, where there are many lipid droplets and mitochondria with well-developed tubular structures, characteristic of steroid-producing cells. When ArKO mice were supplemented with 17beta-oestradiol (E(2); 15 microg/mouse) every fourth day from 4 weeks of age for 1 month, increased numbers of follicles were observed in the ovaries as compared with those of untreated ArKO mice, although no typical corpus luteum was detectable. Ultrastructural analysis revealed the disappearance of the accumulated smooth endoplasmic reticulum in the luteinized interstitial cells after E(2 )supplementation. Transcripts of pro-apoptotic genes such as p53 and Bax genes were markedly elevated in the ArKO ovaries as compared with those of wild-type mice. Although E(2) supplementation did not cause suppression of the elevated expression of p53 and Bax mRNAs, it caused marked enhancement of expression levels of lactoferrin and progesterone receptor mRNAs in the uteri as well as increases in uterine wet weight. At 8 months of age, ArKO mice developed haemorrhages in the ovaries, in which follicles were nearly depleted, while age-matched wild-type females still had many ovarian follicles. Furthermore, macrophage-like cells were occasionally observed in the ArKO ovarian follicles. These results suggested that targeted disruption of Cyp19 caused anovulation and precocious depletion of ovarian follicles. Additionally, analysis of mice supplemented with E(2) demonstrated that E(2) apparently supports development of ovarian follicles, although it did not restore the defect in ovulation.

Histochemical demonstration of hydrogen peroxide production by leukocytes in fixed-frozen tissue sections of inflammatory lesions

1994 ◽  
Vol 56 (4) ◽  
pp. 436-443 ◽  
Author(s):  
Arthur M. Dannenberg ◽  
Brian H. Schofield ◽  
Jay B. Rao ◽  
Theresa T. Dinh ◽  
Ki Lee ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Histochemical Demonstration ◽  
Hydrogen Peroxide Production ◽  
Inflammatory Lesions ◽  
Tissue Sections ◽  
Frozen Tissue

A Technique for the Histochemical Demonstration of Polyphenol Oxidase and its application to Egg-shell Formation in Helminths and Byssus Formation in Mytilus

1954 ◽  
Vol s3-95 (30) ◽  
pp. 139-152
Author(s):  
J. D. SMYTH
Keyword(s):  
Positive Reaction ◽  
Polyphenol Oxidase ◽  
Histochemical Demonstration ◽  
Female Genitalia ◽  
Shell Formation ◽  
Whole Mount ◽  
Vitelline Cells ◽  
Quinone Tanning ◽  
Oxidase Test ◽  
Egg Shell

1. The distribution of polyphenol oxidase in quinone-tanning systems may be demonstrated in frozen-dried sections by incubation in 0.2 per cent, aqueous catechol at 400° C. for 15-60 minutes. A red colour develops at the enzyme site. 2. The evidence for the view that the egg-shell in trematodes, in certain cestode groups, and in turbellarians, is a quinone-tanned protein secreted by the so-called ‘vitelline’ glands, is summarized. The ‘vitelline’ cells, in addition to giving positive reactions for proteins and phenols, give a strongly positive reaction with the catechol polyphenol oxidase test. 3. The catechol technique may also be applied to whole helminths fixed in 70 per cent, alcohol, and serves as a useful whole mount stain for the shell-producing regions of the female genitalia. 4. In Mytilus the catechol technique reveals the presence of polyphenol oxidase in an ‘upper’ or enzyme gland in the foot. 5. It is suggested that in Mytilus the byssus is formed from a phenolic protein secreted from the phenol gland, which on contact with polyphenol oxidase can undergo ‘auto-quinone tanning’.

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