Semipermeable membranes for improving the histochemical demonstration of enzyme activities in tissue sections

1975 ◽  
Vol 43 (3) ◽  
pp. 225-236 ◽  
Author(s):  
A. E. F. H. Meijer ◽  
G. P. de Vries
Keyword(s):  
Enzyme Activities ◽  
Histochemical Demonstration ◽  
Semipermeable Membranes ◽  
Tissue Sections

Histochemical Demonstration of Enzyme Activities in Plastic and Paraffin Embedded Tissue Sections

1979 ◽  
Vol 54 (1) ◽  
pp. 5-12 ◽  
Author(s):  
Sadanobu Higuchi ◽  
Moritaka Suga ◽  
Arthur M. Dannenberg ◽  
Brian H. Schofield
Keyword(s):  
Enzyme Activities ◽  
Histochemical Demonstration ◽  
Tissue Sections

Histochemical Demonstration of Copper and Copper-Associated Protein in the Canine Liver

1985 ◽  
Vol 22 (4) ◽  
pp. 327-332 ◽  
Author(s):  
L. P. Thornburg ◽  
M. Beissenherz ◽  
M. Dolan ◽  
M. F. Raisbeck
Keyword(s):  
Atomic Absorption ◽  
Histochemical Demonstration ◽  
Atomic Absorption Analysis ◽  
Absorption Analysis ◽  
Tissue Sections ◽  
Histochemical Methods ◽  
Histochemical Detection ◽  
Rubeanic Acid ◽  
Copper Detection

Three different histochemical methods for copper detection were compared. Atomic absorption analysis was used to substantiate the tissue stains. There was good correlation between rhodanine staining and rubeanic acid-stained tissue sections. The orcein reaction for copper-associated protein did not consistently correlate with the methods demonstrating copper. Prolonged staining (72 hours) with rubeanic acid more consistently and clearly detected increased copper in canine livers than did staining with rhodanine. Seventy-two hour staining with rubeanic acid is the method of choice for histochemical detection of copper in canine liver.

Histochemical demonstration of hydrogen peroxide production by leukocytes in fixed-frozen tissue sections of inflammatory lesions

1994 ◽  
Vol 56 (4) ◽  
pp. 436-443 ◽  
Author(s):  
Arthur M. Dannenberg ◽  
Brian H. Schofield ◽  
Jay B. Rao ◽  
Theresa T. Dinh ◽  
Ki Lee ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Histochemical Demonstration ◽  
Hydrogen Peroxide Production ◽  
Inflammatory Lesions ◽  
Tissue Sections ◽  
Frozen Tissue

scholarly journals Use of tissue-free glycol methacrylate sections as semi-permeable membranes: a simple way to shorten incubation times and to improve localization in enzyme histochemistry.

1989 ◽  
Vol 37 (2) ◽  
pp. 173-176 ◽  
Author(s):  
P O Gerrits ◽  
R W Horobin ◽  
M J Hardonk
Keyword(s):  
Alkaline Phosphatase ◽  
Azo Dye ◽  
Enzyme Histochemistry ◽  
Diazonium Salt ◽  
Histochemical Staining ◽  
Semipermeable Membranes ◽  
Large Molecules ◽  
Glycol Methacrylate ◽  
Tissue Sections ◽  
Antibody Staining

Placing 2-microns sections of tissue-free glycol methacrylate on top of tissue sections is a simple way of forming semipermeable membranes to enhance enzyme histochemical staining. For demonstrating alkaline phosphatase in glycol methacrylate-embedded kidney by a standard azo dye method, such membranes enabled incubation times to be reduced to 1-2 hr, with azo dye reaction product being more crisply localized as compared to sections stained without membranes. Such effects are possible because the membranes are highly permeable to small molecules (e.g., substrate and diazonium salt), slightly permeable to molecules of moderate size (e.g., the final reaction product), and impermeable to large molecules (e.g., alkaline phosphatase and other tissue biopolymers). The implications of these findings for enzyme histochemistry and for enzyme-labeled antibody staining are discussed.

scholarly journals MICROSCOPIC HISTOCHEMICAL DEMONSTRATION OF STEROID-3β-OL DEHYDROGENASE IN TISSUE SECTIONS

1958 ◽  
Vol 6 (4) ◽  
pp. 225-232 ◽  
Author(s):  
LEE W. WATTENBERG
Keyword(s):  
Corpus Luteum ◽  
Adrenal Cortex ◽  
Positive Reaction ◽  
Interstitial Cells ◽  
Histochemical Demonstration ◽  
Biochemical Data ◽  
Tissue Sections

A procedure for the microscopic histochemical demonstration of steroid-3β-ol dehydrogenase has been described. In agreement with reported quantitative biochemical data, positive reactions have been obtained in the adrenal cortex, corpus luteum, placenta, and interstitial cells of the testis. In addition, a strongly positive reaction is obtained in the interstitial cells of the rodent ovary.

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