scholarly journals Comparison of Hemagglutination Inhibition Assay and Enzyme Immunoassay for Determination of Mumps and Rubella Immune Status in Health Care Personnel

2013 ◽  
Vol 27 (5) ◽  
pp. 418-421 ◽  
Author(s):  
Shunichi Kumakura ◽  
Hiroshi Shibata ◽  
Takeshi Isobe ◽  
Masahiro Hirose ◽  
Miki Ohe ◽  
...  
Keyword(s):  
Health Care ◽  
Enzyme Immunoassay ◽  
Hemagglutination Inhibition ◽  
Immune Status ◽  
Health Care Personnel ◽  
Inhibition Assay ◽  
Hemagglutination Inhibition Assay

scholarly journals Hemagglutination Inhibition Assay for Gentamicin

1977 ◽  
Vol 11 (2) ◽  
pp. 359-361 ◽  
Author(s):  
W. A. Mahon ◽  
R. I. Feldman ◽  
G. H. Scherr
Keyword(s):  
Hemagglutination Inhibition ◽  
Inhibition Assay ◽  
Hemagglutination Inhibition Assay

scholarly journals Standardization of Hemagglutination Inhibition Assay for Influenza Serology Allows for High Reproducibility between Laboratories

2016 ◽  
Vol 23 (3) ◽  
pp. 236-242 ◽  
Author(s):  
Mary Zacour ◽  
Brian J. Ward ◽  
Angela Brewer ◽  
Patrick Tang ◽  
Guy Boivin ◽  
...  
Keyword(s):  
Hemagglutination Inhibition ◽  
Laboratory Data ◽  
Research Network ◽  
Reference Laboratory ◽  
Inhibition Assay ◽  
Serum Samples ◽  
High Reproducibility ◽  
Hemagglutination Inhibition Assay ◽  
Geographically Dispersed

ABSTRACTStandardization of the hemagglutination inhibition (HAI) assay for influenza serology is challenging. Poor reproducibility of HAI results from one laboratory to another is widely cited, limiting comparisons between candidate vaccines in different clinical trials and posing challenges for licensing authorities. In this study, we standardized HAI assay materials, methods, and interpretive criteria across five geographically dispersed laboratories of a multidisciplinary influenza research network and then evaluated intralaboratory and interlaboratory variations in HAI titers by repeatedly testing standardized panels of human serum samples. Duplicate precision and reproducibility from comparisons between assays within laboratories were 99.8% (99.2% to 100%) and 98.0% (93.3% to 100%), respectively. The results for 98.9% (95% to 100%) of the samples were within 2-fold of all-laboratory consensus titers, and the results for 94.3% (85% to 100%) of the samples were within 2-fold of our reference laboratory data. Low-titer samples showed the greatest variability in comparisons between assays and between sites. Classification of seroprotection (titer ≥ 40) was accurate in 93.6% or 89.5% of cases in comparison to the consensus or reference laboratory classification, respectively. This study showed that with carefully chosen standardization processes, high reproducibility of HAI results between laboratories is indeed achievable.

scholarly journals Evaluation of a Multiplex Bead Immunoassay for Determination of Immune Status to Varicella-Zoster Virus in Medical Center Students and Employees

2015 ◽  
Vol 22 (3) ◽  
pp. 351-353 ◽  
Author(s):  
Michael J. Loeffelholz ◽  
Harry E. Prince
Keyword(s):  
Varicella Zoster Virus ◽  
Enzyme Immunoassay ◽  
Predictive Value ◽  
Immune Status ◽  
Medical Center ◽  
Igg Antibodies ◽  
Varicella Zoster ◽  
Multiplex Bead ◽  
Bead Immunoassay

ABSTRACTThis study evaluated an enzyme immunoassay, a multiplex bead immunoassay (MBIA), and the anticomplement immunofluorescence (ACIF) test for detecting varicella-zoster virus IgG antibodies in sera from medical center students and employees. The agreement between methods was ≥95%. The MBIA was less sensitive than was the ACIF test, with a negative predictive value of 66.7%.

Sign in / Sign up

Export Citation Format

Share Document