Changes in Histone Methylation and Acetylation during Microspore Reprogramming to Embryogenesis Occur Concomitantly with BnHKMT and BnHAT Expression and Are Associated with Cell Totipotency, Proliferation, and Differentiation in Brassica napus

2014 ◽  
Vol 143 (1-3) ◽  
pp. 209-218 ◽  
Author(s):  
Héctor Rodríguez-Sanz ◽  
Jordi Moreno-Romero ◽  
María-Teresa Solís ◽  
Claudia Köhler ◽  
María C. Risueño ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Histone Methylation ◽  
Proliferation And Differentiation ◽  
Microspore Reprogramming ◽  
Histone Methylation And Acetylation

scholarly journals SOX19b regulates the premature neuronal differentiation of neural stem cells through EZH2-mediated histone methylation in neural tube development of zebrafish

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Xian Li ◽  
Wenjuan Zhou ◽  
Xinyue Li ◽  
Ming Gao ◽  
Shufang Ji ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Neural Tube ◽  
Neural Tube Defects ◽  
Histone Methylation ◽  
Zebrafish Embryos ◽  
Loss Of Function ◽  
Zebrafish Model ◽  
Proliferation And Differentiation ◽  
Neural Tube Development

Abstract Objective Neural tube defects (NTDs) are the most serious and common birth defects in the clinic. The SRY-related HMG box B1 (SoxB1) gene family has been implicated in different processes of early embryogenesis. Sox19b is a maternally expressed gene in the SoxB1 family that is found in the region of the presumptive central nervous system (CNS), but its role and mechanism in embryonic neural stem cells (NSCs) during neural tube development have not yet been explored. Considering that Sox19b is specific to bony fish, we intended to investigate the role and mechanism of Sox19b in neural tube development in zebrafish embryos. Material and methods Morpholino (MO) antisense oligonucleotides were used to construct a Sox19b loss-of-function zebrafish model. The phenotype and the expression of related genes were analysed by in situ hybridization and immunolabelling. Epigenetic modifications were detected by western blot and chromatin immunoprecipitation. Results In this study, we found that zebrafish embryos exhibited a reduced or even deleted forebrain phenotype after the expression of the Sox19b gene was inhibited. Moreover, we found for the first time that knockdown of Sox19b reduced the proliferation of NSCs; increased the transcription levels of Ngn1, Ascl1, HuC, Islet1, and cyclin-dependent kinase (CDK) inhibitors; and led to premature differentiation of NSCs. Finally, we found that knockdown of Sox19b decreased the levels of EZH2/H3K27me3 and decreased the level of H3K27me3 at the promoters of Ngn1 and ascl1a. Conclusion Together, our data demonstrate that Sox19b plays an essential role in early NSC proliferation and differentiation through EZH2-mediated histone methylation in neural tube development. This study established the role of transcription factor Sox19b and epigenetic factor EZH2 regulatory network on NSC development, which provides new clues and theoretical guidance for the clinical treatment of neural tube defects.

scholarly journals BCL-6 suppresses miR-142-3p/5p expression in SLE CD4+ T cells by modulating histone methylation and acetylation of the miR-142 promoter

2019 ◽  
Vol 17 (5) ◽  
pp. 474-482 ◽  
Author(s):  
Shu Ding ◽  
Qing Zhang ◽  
Shuangyan Luo ◽  
Lihua Gao ◽  
Jinhua Huang ◽  
...  
Keyword(s):  
T Cells ◽  
Histone Methylation ◽  
Cd4 T Cells ◽  
Histone Methylation And Acetylation

scholarly journals Chromatin remodeling in mammalian embryos

Reproduction ◽  
2018 ◽  
Vol 155 (3) ◽  
pp. R147-R158 ◽  
Author(s):  
Birgit Cabot ◽  
Ryan A Cabot
Keyword(s):  
Chromatin Remodeling ◽  
Histone Methylation ◽  
Mammalian Species ◽  
Epigenetic Changes ◽  
Mammalian Embryo ◽  
Mammalian Embryos ◽  
Epigenetic Remodeling ◽  
Histone Methylation And Acetylation

The mammalian embryo undergoes a dramatic amount of epigenetic remodeling during the first week of development. In this review, we discuss several epigenetic changes that happen over the course of cleavage development, focusing on covalent marks (e.g., histone methylation and acetylation) and non-covalent remodeling (chromatin remodeling via remodeling complexes; e.g., SWI/SNF-mediated chromatin remodeling). Comparisons are also drawn between remodeling events that occur in embryos from a variety of mammalian species.

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