Arxula adeninivorans Recombinant Guanine Deaminase and Its Application in the Production of Food with Low Purine Content

2014 ◽  
Vol 24 (2) ◽  
pp. 67-81 ◽  
Author(s):  
Anke Trautwein-Schult ◽  
Dagmara Jankowska ◽  
Arno Cordes ◽  
Petra Hoferichter ◽  
Christina Klein ◽  
...  
Keyword(s):  
Arxula Adeninivorans ◽  
Guanine Deaminase ◽  
Purine Content

Arxula adeninivorans recombinant adenine deaminase and its application in the production of food with low purine content

2013 ◽  
Vol 115 (5) ◽  
pp. 1134-1146 ◽  
Author(s):  
D.A. Jankowska ◽  
K. Faulwasser ◽  
A. Trautwein-Schult ◽  
A. Cordes ◽  
P. Hoferichter ◽  
...  
Keyword(s):  
Arxula Adeninivorans ◽  
Purine Content ◽  
Adenine Deaminase

scholarly journals Arxula adeninivorans xanthine oxidoreductase and its application in the production of food with low purine content

2013 ◽  
Vol 115 (3) ◽  
pp. 796-807 ◽  
Author(s):  
D.A. Jankowska ◽  
A. Trautwein-Schult ◽  
A. Cordes ◽  
P. Hoferichter ◽  
C. Klein ◽  
...  
Keyword(s):  
Arxula Adeninivorans ◽  
Xanthine Oxidoreductase ◽  
Purine Content

scholarly journals A novel enzymatic approach in the production of food with low purine content usingArxula adeninivoransendogenous and recombinant purine degradative enzymes

Bioengineered ◽  
2015 ◽  
Vol 6 (1) ◽  
pp. 20-25 ◽  
Author(s):  
Dagmara A Jankowska ◽  
Anke Trautwein-Schult ◽  
Arno Cordes ◽  
Rüdiger Bode ◽  
Keith Baronian ◽  
...  
Keyword(s):  
Purine Content ◽  
Degradative Enzymes

scholarly journals Improved Production of Heterologous Proteins by a Glucose Repression-Defective Mutant of Kluyveromyces lactis

2004 ◽  
Vol 70 (5) ◽  
pp. 2632-2638 ◽  
Author(s):  
Claudia Donnini ◽  
Francesca Farina ◽  
Barbara Neglia ◽  
Maria Concetta Compagno ◽  
Daniela Uccelletti ◽  
...  
Keyword(s):  
Glucose Repression ◽  
Kluyveromyces Lactis ◽  
Kinase Domain ◽  
Arxula Adeninivorans ◽  
Nucleotide Position ◽  
Heterologous Proteins ◽  
Reporter Protein ◽  
Gene Encoding ◽  
Defective Mutant ◽  
Highly Sensitive

ABSTRACT The secreted production of heterologous proteins in Kluyveromyces lactis was studied. A glucoamylase (GAA) from the yeast Arxula adeninivorans was used as a reporter protein for the study of the secretion efficiencies of several wild-type and mutant strains of K. lactis. The expression of the reporter protein was placed under the control of the strong promoter of the glyceraldehyde-3-phosphate dehydrogenase of Saccharomyces cerevisiae. Among the laboratory strains tested, strain JA6 was the best producer of GAA. Since this strain is known to be highly sensitive to glucose repression and since this is an undesired trait for biomass-oriented applications, we examined heterologous protein production by using glucose repression-defective mutants isolated from this strain. One of them, a mutant carrying a dgr151-1 mutation, showed a significantly improved capability of producing heterologous proteins such as GAA, human serum albumin, and human interleukin-1β compared to the parent strain. dgr151-1 is an allele of RAG5, the gene encoding the only hexokinase present in K. lactis (a homologue of S. cerevisiae HXK2). The mutation in this strain was mapped to nucleotide position +527, resulting in a change from glycine to aspartic acid within the highly conserved kinase domain. Cells carrying the dgr151-1 allele also showed a reduction in N- and O-glycosylation. Therefore, the dgr151 strain may be a promising host for the production of heterologous proteins, especially when the hyperglycosylation of recombinant proteins must be avoided.

scholarly journals Crystal Structure of Bacillus subtilis Guanine Deaminase

2004 ◽  
Vol 279 (34) ◽  
pp. 35479-35485 ◽  
Author(s):  
Shwu-Huey Liaw ◽  
Yu-Jui Chang ◽  
Cheng-Tsung Lai ◽  
Hui-Chuan Chang ◽  
Gu-Gang Chang
Keyword(s):  
Crystal Structure ◽  
Bacillus Subtilis ◽  
Guanine Deaminase

Insights into the Dual Shuttle Catalytic Mechanism of Guanine Deaminase

2021 ◽  
Author(s):  
Asmita Sen ◽  
Vandana Gaded ◽  
Prabha Jayapal ◽  
Gopalan Rajaraman ◽  
Ruchi Anand
Keyword(s):  
Catalytic Mechanism ◽  
Guanine Deaminase

Endothelial Purine Content

1998 ◽  
pp. 827-831 ◽  
Author(s):  
H. Vorbach ◽  
G. Weigel ◽  
B. Robibaro ◽  
M. Reiter ◽  
M. Hlousek ◽  
...  
Keyword(s):  
Purine Content

scholarly journals Upregulated Guanine Deaminase Is Involved in Hyperpigmentation of Seborrheic Keratosis via Uric Acid Release

2021 ◽  
Vol 22 (22) ◽  
pp. 12501
Author(s):  
Kyung Ah Cheong ◽  
In Sup Kil ◽  
Hyuk Wan Ko ◽  
Ai-Young Lee
Keyword(s):  
Uric Acid ◽  
The Elderly ◽  
Epidermal Keratinocytes ◽  
Seborrheic Keratosis ◽  
Human Epidermal Keratinocytes ◽  
Skin Hyperpigmentation ◽  
Guanine Deaminase ◽  
Acid Release ◽  
Skin Specimen

Seborrheic keratosis, which is a benign tumor composed of epidermal keratinocytes, develops common in the elderly. Uric acid generated by upregulated guanine deaminase (GDA) has been identified to cause UV-induced keratinocyte senescence in seborrheic keratosis. Seborrheic keratosis is also frequently pigmented. Growing evidences indicate that hyperuricemia is a risk factor of acanthosis nigricans, an acquired skin hyperpigmentation. The objective of this study was to investigate role of GDA and its metabolic end product, uric acid, in hyperpigmentation of patients with seborrheic keratosis using their lesional and non-lesional skin specimen sets and cultured primary human epidermal keratinocytes with or without GDA overexpression or uric acid treatment. GDA-overexpressing keratinocytes or their conditioned media containing uric acid increased expression levels of MITF and tyrosinase in melanocytes. Uric acid released from keratinocytes was facilitated by ABCG2 transporter with the help of PDZK1 interaction. Released uric acid was taken by URAT1 transporter in melanocytes, stimulating melanogenesis through p38 MAPK activation. Overall, GDA upregulation in seborrheic keratosis plays a role in melanogenesis via its metabolic end product uric acid, suggesting that seborrheic keratosis as an example of hyperpigmentation associated with photoaging.

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