Arxula adeninivorans recombinant adenine deaminase and its application in the production of food with low purine content

2013 ◽  
Vol 115 (5) ◽  
pp. 1134-1146 ◽  
Author(s):  
D.A. Jankowska ◽  
K. Faulwasser ◽  
A. Trautwein-Schult ◽  
A. Cordes ◽  
P. Hoferichter ◽  
...  
Keyword(s):  
Arxula Adeninivorans ◽  
Purine Content ◽  
Adenine Deaminase

scholarly journals Arxula adeninivorans xanthine oxidoreductase and its application in the production of food with low purine content

2013 ◽  
Vol 115 (3) ◽  
pp. 796-807 ◽  
Author(s):  
D.A. Jankowska ◽  
A. Trautwein-Schult ◽  
A. Cordes ◽  
P. Hoferichter ◽  
C. Klein ◽  
...  
Keyword(s):  
Arxula Adeninivorans ◽  
Xanthine Oxidoreductase ◽  
Purine Content

Arxula adeninivorans Recombinant Guanine Deaminase and Its Application in the Production of Food with Low Purine Content

2014 ◽  
Vol 24 (2) ◽  
pp. 67-81 ◽  
Author(s):  
Anke Trautwein-Schult ◽  
Dagmara Jankowska ◽  
Arno Cordes ◽  
Petra Hoferichter ◽  
Christina Klein ◽  
...  
Keyword(s):  
Arxula Adeninivorans ◽  
Guanine Deaminase ◽  
Purine Content

scholarly journals A novel enzymatic approach in the production of food with low purine content usingArxula adeninivoransendogenous and recombinant purine degradative enzymes

Bioengineered ◽  
2015 ◽  
Vol 6 (1) ◽  
pp. 20-25 ◽  
Author(s):  
Dagmara A Jankowska ◽  
Anke Trautwein-Schult ◽  
Arno Cordes ◽  
Rüdiger Bode ◽  
Keith Baronian ◽  
...  
Keyword(s):  
Purine Content ◽  
Degradative Enzymes

scholarly journals Cytosine and adenine deaminase base-editors induce broad and nonspecific changes in gene expression and splicing

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Jiao Fan ◽  
Yige Ding ◽  
Chao Ren ◽  
Ziguo Song ◽  
Jie Yuan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Double Strand Breaks ◽  
Great Promise ◽  
Single Nucleotide ◽  
Strand Breaks ◽  
Dna Base ◽  
Target Dna ◽  
Single Nucleotide Variations ◽  
Adenine Deaminase ◽  
Target Rna

AbstractCytosine or adenine base editors (CBEs or ABEs) hold great promise in therapeutic applications because they enable the precise conversion of targeted base changes without generating of double-strand breaks. However, both CBEs and ABEs induce substantial off-target DNA editing, and extensive off-target RNA single nucleotide variations in transfected cells. Therefore, the potential effects of deaminases induced by DNA base editors are of great importance for their clinical applicability. Here, the transcriptome-wide deaminase effects on gene expression and splicing is examined. Differentially expressed genes (DEGs) and differential alternative splicing (DAS) events, induced by base editors, are identified. Both CBEs and ABEs generated thousands of DEGs and hundreds of DAS events. For engineered CBEs or ABEs, base editor-induced variants had little effect on the elimination of DEGs and DAS events. Interestingly, more DEGs and DAS events are observed as a result of over expressions of cytosine and adenine deaminases. This study reveals a previously overlooked aspect of deaminase effects in transcriptome-wide gene expression and splicing, and underscores the need to fully characterize such effects of deaminase enzymes in base editor platforms.

Effects of iron supplementation on blood adenine deaminase activity and oxidative stress in Trypanosoma evansi infection of rats

2014 ◽  
Vol 147 ◽  
pp. 1-6 ◽  
Author(s):  
Nathieli B. Bottari ◽  
Matheus D. Baldissera ◽  
Alexandre A. Tonin ◽  
Raqueli T. França ◽  
Danieli Zanini ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Iron Supplementation ◽  
Trypanosoma Evansi ◽  
Adenine Deaminase ◽  
And Oxidative Stress

scholarly journals Improved Production of Heterologous Proteins by a Glucose Repression-Defective Mutant of Kluyveromyces lactis

2004 ◽  
Vol 70 (5) ◽  
pp. 2632-2638 ◽  
Author(s):  
Claudia Donnini ◽  
Francesca Farina ◽  
Barbara Neglia ◽  
Maria Concetta Compagno ◽  
Daniela Uccelletti ◽  
...  
Keyword(s):  
Glucose Repression ◽  
Kluyveromyces Lactis ◽  
Kinase Domain ◽  
Arxula Adeninivorans ◽  
Nucleotide Position ◽  
Heterologous Proteins ◽  
Reporter Protein ◽  
Gene Encoding ◽  
Defective Mutant ◽  
Highly Sensitive

ABSTRACT The secreted production of heterologous proteins in Kluyveromyces lactis was studied. A glucoamylase (GAA) from the yeast Arxula adeninivorans was used as a reporter protein for the study of the secretion efficiencies of several wild-type and mutant strains of K. lactis. The expression of the reporter protein was placed under the control of the strong promoter of the glyceraldehyde-3-phosphate dehydrogenase of Saccharomyces cerevisiae. Among the laboratory strains tested, strain JA6 was the best producer of GAA. Since this strain is known to be highly sensitive to glucose repression and since this is an undesired trait for biomass-oriented applications, we examined heterologous protein production by using glucose repression-defective mutants isolated from this strain. One of them, a mutant carrying a dgr151-1 mutation, showed a significantly improved capability of producing heterologous proteins such as GAA, human serum albumin, and human interleukin-1β compared to the parent strain. dgr151-1 is an allele of RAG5, the gene encoding the only hexokinase present in K. lactis (a homologue of S. cerevisiae HXK2). The mutation in this strain was mapped to nucleotide position +527, resulting in a change from glycine to aspartic acid within the highly conserved kinase domain. Cells carrying the dgr151-1 allele also showed a reduction in N- and O-glycosylation. Therefore, the dgr151 strain may be a promising host for the production of heterologous proteins, especially when the hyperglycosylation of recombinant proteins must be avoided.

Purine salvage inMethanocaldococcus jannaschii: Elucidating the role of a conserved cysteine in adenine deaminase

2016 ◽  
Vol 84 (6) ◽  
pp. 828-840 ◽  
Author(s):  
Danielle V. Miller ◽  
Anne M. Brown ◽  
Huimin Xu ◽  
David R. Bevan ◽  
Robert H. White
Keyword(s):  
Purine Salvage ◽  
Adenine Deaminase

Endothelial Purine Content

1998 ◽  
pp. 827-831 ◽  
Author(s):  
H. Vorbach ◽  
G. Weigel ◽  
B. Robibaro ◽  
M. Reiter ◽  
M. Hlousek ◽  
...  
Keyword(s):  
Purine Content
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