Equine platelet lysate as an alternative to fetal bovine serum in equine mesenchymal stromal cell culture - too much of a good thing?

2015 ◽  
Vol 48 (2) ◽  
pp. 261-264 ◽  
Author(s):  
K. A. Russell ◽  
T. G. Koch
Keyword(s):  
Cell Culture ◽  
Bovine Serum ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Fetal Bovine Serum ◽  
Platelet Lysate ◽  
Good Thing ◽  
Stromal Cell Culture ◽  
Fetal Bovine

Serum-converted platelet lysate can substitute for fetal bovine serum in human mesenchymal stromal cell cultures

Cytotherapy ◽  
2013 ◽  
Vol 15 (12) ◽  
pp. 1458-1468 ◽  
Author(s):  
Mariluz P. Mojica-Henshaw ◽  
Pam jacobson ◽  
Julie Morris ◽  
Linda Kelley ◽  
Jan Pierce ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Bovine Serum ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Fetal Bovine Serum ◽  
Platelet Lysate ◽  
Fetal Bovine

scholarly journals Platelet lysate: a replacement for fetal bovine serum in animal cell culture?

2003 ◽  
Vol 42 (2) ◽  
pp. 67-74 ◽  
Author(s):  
Liselott Johansson ◽  
Jeanna Klinth ◽  
Olov Holmqvist ◽  
Sten Ohlson
Keyword(s):  
Cell Culture ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Animal Cell ◽  
Animal Cell Culture ◽  
Platelet Lysate ◽  
Fetal Bovine

scholarly journals Platelet Lysate for Mesenchymal Stromal Cell Culture in the Canine and Equine Species: Analogous but Not the Same

Animals ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 189
Author(s):  
Alina Hagen ◽  
Heidrun Holland ◽  
Vivian-Pascal Brandt ◽  
Carla U. Doll ◽  
Thomas C. Häußler ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β1 ◽  
Buffy Coat ◽  
Platelet Lysate ◽  
Stromal Cell Culture ◽  
Fetal Bovine ◽  
Free Replacement ◽  
Apoptosis And Necrosis

Platelet lysate (PL) is an attractive platelet-based therapeutic tool and has shown promise as xeno-free replacement for fetal bovine serum (FBS) in human and equine mesenchymal stromal cell (MSC) culture. Here, we established a scalable buffy-coat-based protocol for canine PL (cPL) production (n = 12). The cPL was tested in canine adipose MSC (n = 5) culture compared to FBS. For further comparison, equine adipose MSC (n = 5) were cultured with analogous equine PL (ePL) or FBS. During canine blood processing, platelet and transforming growth factor-β1 concentrations increased (p < 0.05 and p < 0.001), while white blood cell concentrations decreased (p < 0.05). However, while equine MSC showed good results when cultured with 10% ePL, canine MSC cultured with 2.5% or 10% cPL changed their morphology and showed decreased metabolic activity (p < 0.05). Apoptosis and necrosis in canine MSC were increased with 2.5% cPL (p < 0.05). Surprisingly, passage 5 canine MSC showed less genetic aberrations after culture with 10% cPL than with FBS. Our data reveal that using analogous canine and equine biologicals does not entail the same results. The buffy-coat-based cPL was not adequate for canine MSC culture, but may still be useful for therapeutic applications.

Human platelet lysate: Replacing fetal bovine serum as a gold standard for human cell propagation?

Biomaterials ◽  
2016 ◽  
Vol 76 ◽  
pp. 371-387 ◽  
Author(s):  
Thierry Burnouf ◽  
Dirk Strunk ◽  
Mickey B.C. Koh ◽  
Katharina Schallmoser
Keyword(s):  
Human Cell ◽  
Gold Standard ◽  
Bovine Serum ◽  
Human Platelet ◽  
Fetal Bovine Serum ◽  
Platelet Lysate ◽  
Human Platelet Lysate ◽  
Fetal Bovine ◽  
Cell Propagation

241 9.22 Performance of Gamma-irradiated Fetal Bovine Serum in Cell Culture

1994 ◽  
pp. 99-101
Author(s):  
J.D. Keathley ◽  
D. Wyatt ◽  
C.M. Williams ◽  
R. Festen ◽  
C. Maben
Keyword(s):  
Cell Culture ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Fetal Bovine ◽  
Gamma Irradiated

scholarly journals Elucidation of the Interaction between Flavan-3-ols and Bovine Serum Albumin and Its Effect on Their In-Vitro Cytotoxicity

Molecules ◽  
2019 ◽  
Vol 24 (20) ◽  
pp. 3667
Author(s):  
Yasuyuki Fujii ◽  
Yoshitomo Suhara ◽  
Yusuke Sukikara ◽  
Tomohiro Teshima ◽  
Yoshihisa Hirota ◽  
...  
Keyword(s):  
Cell Culture ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
In Vitro Cytotoxicity ◽  
Binding Energies ◽  
Docking Simulations ◽  
Fetal Bovine

Flavan-3-ols (FLs), specifically catechin and its oligomer B-type procyanidins, are suggested to potently bind to bovine serum albumin (BSA). We examined the interaction between BSA and FLs by fluorescence quenching and found the following order of binding activities to BSA: cinnamtannin A2 (A2; tetramer) > procyanidin C1 (C1; trimer) ≈ procyanidin B2 (B2, dimer) > (−)epicatechin (EC, monomer). Docking simulations between BSA and each compound at the binding site showed that the calculated binding energies were consistent with the results of our experimental assay. FLs exerted cytotoxicity at 1000 μg/mL in F11 cell culture with fetal bovine serum containing BSA. In culture containing serum-free medium, FLs exhibited significant cell proliferation at 10−4 μg/mL and cytotoxicity was observed at concentrations greater than 10 μg/mL. Results of this study suggest that interactions between polyphenols and BSA should be taken into account when evaluating procyanidin in an in vitro cell culture system.

Detection of bovine viruses in fetal bovine serum used in cell culture

In Vitro ◽  
1975 ◽  
Vol 11 (6) ◽  
pp. 400-403 ◽  
Author(s):  
A. J. Kniazeff ◽  
L. J. Wopschall ◽  
H. E. Hopps ◽  
C. S. Morris
Keyword(s):  
Cell Culture ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Fetal Bovine ◽  
Bovine Viruses

scholarly journals Human Platelet Lysate as a Functional Substitute for Fetal Bovine Serum in the Culture of Human Adipose Derived Stromal/Stem Cells

Cells ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 724 ◽  
Author(s):  
Mathew Cowper ◽  
Trivia Frazier ◽  
Xiying Wu ◽  
J. Curley ◽  
Michelle Ma ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Human Platelets ◽  
Dependent Manner ◽  
Wide Range ◽  
Human Ascs ◽  
Fetal Bovine ◽  
Stromal Stem Cells

Introduction: Adipose derived stromal/stem cells (ASCs) hold potential as cell therapeutics for a wide range of disease states; however, many expansion protocols rely on the use of fetal bovine serum (FBS) as a cell culture nutrient supplement. The current study explores the substitution of lysates from expired human platelets (HPLs) as an FBS substitute. Methods: Expired human platelets from an authorized blood center were lysed by freeze/thawing and used to examine human ASCs with respect to proliferation using hematocytometer cell counts, colony forming unit-fibroblast (CFU-F) frequency, surface immunophenotype by flow cytometry, and tri-lineage (adipocyte, chondrocyte, osteoblast) differentiation potential by histochemical staining. Results: The proliferation assays demonstrated that HPLs supported ASC proliferation in a concentration dependent manner, reaching levels that exceeded that observed in the presence of 10% FBS. The concentration of 0.75% HPLs was equivalent to 10% FBS when utilized in cell culture media with respect to proliferation, immunophenotype, and CFU-F frequency. When added to osteogenic, adipogenic, and chondrogenic differentiation media, both supplements showed appropriate differentiation by staining. Conclusion: HPLs is an effective substitute for FBS in the culture, expansion and differentiation of human ASCs suitable for pre-clinical studies; however, additional assays and analyses will be necessary to validate HPLs for clinical applications and regulatory approval.

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