scholarly journals Comparison of Platelet-Rich Plasma and VEGF-Transfected Mesenchymal Stem Cells on Vascularization and Bone Formation in a Critical-Size Bone Defect

2012 ◽  
Vol 196 (6) ◽  
pp. 523-533 ◽  
Author(s):  
P. Kasten ◽  
M. Beverungen ◽  
H. Lorenz ◽  
J. Wieland ◽  
M. Fehr ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Bone Defect ◽  
Critical Size ◽  
Platelet Rich Plasma

Isolation and Implantation of Bone Marrow–Derived Mesenchymal Stem Cells with Fibrin Micro Beads to Repair a Critical-Size Bone Defect in Mice

2009 ◽  
Vol 15 (9) ◽  
pp. 2537-2546 ◽  
Author(s):  
Alon Ben-Ari ◽  
Rachel Rivkin ◽  
Miryam Frishman ◽  
Elena Gaberman ◽  
Lilia Levdansky ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bone Defect ◽  
Critical Size

Mesenchymal stem cells and platelet-rich plasma enhance bone formation in sinus grafting: a histomorphometric study in minipigs

2008 ◽  
Vol 35 (6) ◽  
pp. 539-546 ◽  
Author(s):  
Francesco Pieri ◽  
Enrico Lucarelli ◽  
Giuseppe Corinaldesi ◽  
Giovanna Iezzi ◽  
Adriano Piattelli ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Platelet Rich Plasma ◽  
Histomorphometric Study ◽  
Sinus Grafting ◽  
Enhance Bone Formation

scholarly journals Effects of Autologous Bone Marrow Mesenchymal Stem Cells and Platelet-Rich Plasma on Bone Regeneration and Osseointegration of A Hydroxyapatite-Coated Titanium Implant

Coatings ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 840
Author(s):  
Francesca Salamanna ◽  
Nicolandrea Del Piccolo ◽  
Maria Sartori ◽  
Gianluca Giavaresi ◽  
Lucia Martini ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Bone Regeneration ◽  
Platelet Rich Plasma ◽  
Autologous Bone Marrow ◽  
Bone Formation Rate ◽  
Marrow Mesenchymal Stem Cells ◽  
Autologous Bone

Bone regeneration remains one of the major clinical needs in orthopedics, and advanced and alternative strategies involving bone substitutes, cells, and growth factors (GFs) are mandatory. The purpose of this study was to evaluate whether the association of autologous bone marrow mesenchymal stem cells (BMSC), isolated by ‘one-step surgical procedure’, and activated platelet rich plasma (PRP) improves osseointegration and bone formation of a hydroxyapatite-coated titanium (Ti-HA) implant, already in clinical use, in a rabbit cancellous defect. The GFs present in plasma, in inactivated and activated PRP were also tested. At 2 weeks, histology and histomorphometry highlighted increased bone-to-implant contact (BIC) in Ti-HA combined with BMSC and PRP in comparison to Ti-HA alone and Ti-HA + PRP. The combined effect of BMSC and PRP peaked at 4 weeks where the BIC value was higher than all other treatments. At both experimental times, newly formed bone (Trabecular Bone Volume, BV/TV) in all tested treatments showed increased values in comparison to Ti-HA alone. At 4 weeks Ti-HA + PRP + BMSC showed the highest BV/TV and the highest osteoblasts number; additionally, a higher osteoid surface and bone formation rate were found in Ti-HA + BMSC + PRP than in all other treatments. Finally, the analyses of GFs revealed higher values in the activated PRP in comparison to plasma and to non-activated PRP. The study suggests that the combination of autologous activated PRP, as a carrier for BMSCs, is a promising regenerative strategy for bone formation, osseointegration, and mineralization of bone implants.

scholarly journals Integration of Umbilical Cord Mesenchymal Stem Cell Application in Hydroxyapatite-Based Scaffolds in the Treatment of Vertebral Bone Defect due to Spondylitis Tuberculosis: A Translational Study

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Ahmad Jabir Rahyussalim ◽  
Ahmad Nugroho ◽  
Muhammad Luqman Labib Zufar ◽  
Irfan Fathurrahman ◽  
Tri Kurniawati
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Umbilical Cord ◽  
Bone Defect ◽  
Developed Countries ◽  
Anterior Fusion ◽  
Lumbar Region ◽  
Vertebral Bone

Background. Vertebral bone defect represents one of the most commonly found skeletal problems in the spine. Progressive increase of vertebral involvement of skeletal tuberculosis (TB) is reported as the main cause, especially in developed countries. Conventional spinal fusion using bone graft has been associated with donor-site morbidity and complications. We reported the utilization of umbilical cord mesenchymal stem cells (UC-MSCs) combined with hydroxyapatite (HA) based scaffolds in treating vertebral bone defect due to spondylitis tuberculosis. Materials and Methods. Three patients with tuberculous spondylitis in the thoracic, thoracolumbar, or lumbar region with vertebral body collapse of more than 50 percent were included. The patient underwent a 2-stage surgical procedure, consisting of debridement, decompression, and posterior stabilization in the first stage followed by anterior fusion using the lumbotomy approach at the second stage. Twenty million UC-MSCs combined with HA granules in 2 cc of saline were transplanted to fill the vertebral bone defect. Postoperative alkaline phosphatase level, quality of life, and radiological healing were evaluated at one-month, three-month, and six-month follow-up. Results. The initial mean ALP level at one-month follow-up was 48.33 ± 8.50   U / L . This value increased at the three-month follow-up but decreased at the six-month follow-up time, 97 ± 8.19   U / L and 90.33 ± 4.16   U / L , respectively. Bone formation of 50-75% of the defect site with minimal fracture line was found. Increased bone formation comprising 75-100% of the total bone area was reported six months postoperation. A total score of the SF-36 questionnaire showed better progression in all 8 domains during the follow-up with the mean total score at six months of 2912.5 ± 116.67 from all patients. Conclusion. Umbilical cord mesenchymal stem cells combined with hydroxyapatite-based scaffold utilization represent a prospective alternative therapy for bone formation and regeneration of vertebral bone defect due to spondylitis tuberculosis. Further clinical investigations are needed to evaluate this new alternative.

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