Comparison capacity of collagen hydrogel, mix‐powder and in situ hydroxyapatite/collagen hydrogelscaffolds with and without mesenchymal stem cells and platelet‐rich plasma in regeneration of critical sized bone defect in a rabbit animal model

2021 ◽  
Author(s):  
Behnam Bakhtiarimoghadam ◽  
Sadegh Shirian ◽  
Esmaeil Mirzaei ◽  
Siavash Sharifi ◽  
Iraj Karimi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Animal Model ◽  
Mesenchymal Stem Cells ◽  
Bone Defect ◽  
Platelet Rich Plasma ◽  
Collagen Hydrogel ◽  
Rabbit Animal Model

scholarly journals Repair of rotator cuff injury in rabbit animal model by in situ implantation of an injectable elastin-like recombinamer and allogeneic mesenchymal stem cells

2016 ◽  
Vol 4 ◽  
Author(s):  
Girotti Alessandra ◽  
Iba�ez Arturo ◽  
Trigueros Larrea Jos� Mar�a ◽  
Lamus Molina Jos� Francisco ◽  
Alonso Matilde ◽  
...  
Keyword(s):  
Stem Cells ◽  
Animal Model ◽  
Mesenchymal Stem Cells ◽  
Rotator Cuff ◽  
Rotator Cuff Injury ◽  
Rabbit Animal Model

scholarly journals Cartilage Repair with Mesenchymal Stem Cells (MSCs) Delivered in a Novel Chondroitin Sulfate / Polyethylene Glycol Hydrogel in a Rabbit Animal Model”

2017 ◽  
Vol 5 (7_suppl6) ◽  
pp. 2325967117S0022 ◽  
Author(s):  
Cecilia Pascual-Garrido ◽  
Francisco Rodriguez Fontan ◽  
Jorge Chahla ◽  
Karin Payne ◽  
Elizabeth Aisenbrey ◽  
...  
Keyword(s):  
Stem Cells ◽  
Animal Model ◽  
Mesenchymal Stem Cells ◽  
Polyethylene Glycol ◽  
Chondroitin Sulfate ◽  
Cartilage Repair ◽  
Rabbit Animal Model

scholarly journals Comparison of Platelet-Rich Plasma and VEGF-Transfected Mesenchymal Stem Cells on Vascularization and Bone Formation in a Critical-Size Bone Defect

2012 ◽  
Vol 196 (6) ◽  
pp. 523-533 ◽  
Author(s):  
P. Kasten ◽  
M. Beverungen ◽  
H. Lorenz ◽  
J. Wieland ◽  
M. Fehr ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Bone Defect ◽  
Critical Size ◽  
Platelet Rich Plasma

scholarly journals Reconstruction of Bone Defect Combined with Massive Loss of Periosteum Using Injectable Human Mesenchymal Stem Cells in Biocompatible Ceramic Scaffolds in a Porcine Animal Model

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Chun-Cheng Lin ◽  
Shih-Chieh Lin ◽  
Chao-Ching Chiang ◽  
Ming-Chau Chang ◽  
Oscar Kuang-Sheng Lee
Keyword(s):  
Stem Cells ◽  
Animal Model ◽  
Mesenchymal Stem Cells ◽  
Bone Defect ◽  
Soft Tissues ◽  
Bone Defects ◽  
Open Fractures ◽  
Large Animal ◽  
Ceramic Scaffold ◽  
Segmental Bone

Clinically, in patients who sustain severe open fractures, there is not only a segmental bone defect needed to be reconstructed but also insufficient healing capacity due to concomitant damages to the periosteum and surrounding soft tissues. For studying the reconstruction of bone defects associated with massive loss of periosteum and surrounding soft tissues, there are no well-established preclinical models in large animals in the literature. The purpose of the study was to generate a large animal model of bone defect with massive periosteum loss and to adopt a tissue engineering approach to achieve rapid bony union with stem cells and biomaterials. In this study, a bone defect with massive periosteum stripping was generated in pigs, which was followed by emptying nearby canal marrow including fat and cancellous bone. The stripped periosteum was a mimic to the situation in the Gustilo type 3 open fractures. Bone defects were then reconstructed by impacting the biocompatible ceramic scaffold, morselized tricalcium phosphate (TCP) loaded with human adipose tissue-derived mesenchymal stem cells (hMSCs). Radiological and pathological assessments indicated that TCP and hMSCs synergistically promoted bone healing with increased lamination and ingrowth of vessels. Both bridging periosteum formation and gap filling were induced rapidly. In conclusion, a porcine model of segmental bone loss with damage of surrounding periosteum was created. Reconstruction of such defects with hMSCs and TCP achieved rapid union of bone defects associated with massive periosteal stripping.

Sign in / Sign up

Export Citation Format

Share Document