scholarly journals Replicative Aging Induces Endothelial to Mesenchymal Transition in Human Aortic Endothelial Cells: Potential Role of Inflammation

2012 ◽  
Vol 49 (1) ◽  
pp. 59-64 ◽  
Author(s):  
Bradley S. Fleenor ◽  
Kurt D. Marshall ◽  
Catarina Rippe ◽  
Douglas R. Seals
Keyword(s):  
Endothelial Cells ◽  
Potential Role ◽  
Replicative Aging ◽  
Aortic Endothelial Cells ◽  
Mesenchymal Transition ◽  
Endothelial To Mesenchymal Transition ◽  
Human Aortic Endothelial Cells ◽  
Aortic Endothelial

Functional expression of Kir2.x in human aortic endothelial cells: the dominant role of Kir2.2

2005 ◽  
Vol 289 (5) ◽  
pp. C1134-C1144 ◽  
Author(s):  
Yun Fang ◽  
Gernot Schram ◽  
Victor G. Romanenko ◽  
Congzhu Shi ◽  
Lisa Conti ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Dominant Role ◽  
Functional Expression ◽  
Inward Rectifier ◽  
Dominant Negative ◽  
Current Sensitivity ◽  
Aortic Endothelial Cells ◽  
Human Aortic Endothelial Cells ◽  
Aortic Endothelial

Inward rectifier K+ channels (Kir) are a significant determinant of endothelial cell (EC) membrane potential, which plays an important role in endothelium-dependent vasodilatation. In the present study, several complementary strategies were applied to determine the Kir2 subunit composition of human aortic endothelial cells (HAECs). Expression levels of Kir2.1, Kir2.2, and Kir2.4 mRNA were similar, whereas Kir2.3 mRNA expression was significantly weaker. Western blot analysis showed clear Kir2.1 and Kir2.2 protein expression, but Kir2.3 protein was undetectable. Functional analysis of endothelial inward rectifier K+ current ( IK) demonstrated that 1) IK current sensitivity to Ba2+ and pH were consistent with currents determined using Kir2.1 and Kir2.2 but not Kir2.3 and Kir2.4, and 2) unitary conductance distributions showed two prominent peaks corresponding to known unitary conductances of Kir2.1 and Kir2.2 channels with a ratio of ∼4:6. When HAECs were transfected with dominant-negative (dn)Kir2.x mutants, endogenous current was reduced ∼50% by dnKir2.1 and ∼85% by dnKir2.2, whereas no significant effect was observed with dnKir2.3 or dnKir2.4. These studies suggest that Kir2.2 and Kir2.1 are primary determinants of endogenous K+ conductance in HAECs under resting conditions and that Kir2.2 provides the dominant conductance in these cells.

The Role of Rho/Rho-Kinase Pathway in the Expression of ICAM-1 by Linoleic Acid in Human Aortic Endothelial Cells

Inflammation ◽  
2011 ◽  
Vol 35 (3) ◽  
pp. 1041-1048 ◽  
Author(s):  
Chang Hee Jung ◽  
Woo Je Lee ◽  
Jenie Yoonoo Hwang ◽  
So Mi Seol ◽  
Yun Mi Kim ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Linoleic Acid ◽  
Rho Kinase ◽  
Aortic Endothelial Cells ◽  
Human Aortic Endothelial Cells ◽  
Kinase Pathway ◽  
Aortic Endothelial
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