scholarly journals Critical Role of NADPH Oxidase-derived Reactive Oxygen Species in Generating Ca2+ Oscillations in Human Aortic Endothelial Cells Stimulated by Histamine

2002 ◽  
Vol 277 (36) ◽  
pp. 32546-32551 ◽  
Author(s):  
Qinghua Hu ◽  
Zu-Xi Yu ◽  
Victor J. Ferrans ◽  
Kazuyo Takeda ◽  
Kaikobad Irani ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Endothelial Cells ◽  
Nadph Oxidase ◽  
Critical Role ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Aortic Endothelial Cells ◽  
Human Aortic Endothelial Cells ◽  
Aortic Endothelial

Hyperglycemia and reactive oxygen species mediate apoptosis in aortic endothelial cells through Janus kinase 2

2005 ◽  
Vol 43 (5) ◽  
pp. 320-326 ◽  
Author(s):  
Amany Tawfik ◽  
Liming Jin ◽  
Amy K.L. Banes-Berceli ◽  
Ruth B. Caldwell ◽  
Safia Ogbi ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Endothelial Cells ◽  
Reactive Oxygen ◽  
Janus Kinase ◽  
Oxygen Species ◽  
Janus Kinase 2 ◽  
Aortic Endothelial Cells ◽  
Aortic Endothelial

Oxidized phospholipids mediate occludin expression and phosphorylation in vascular endothelial cells

2006 ◽  
Vol 290 (2) ◽  
pp. H674-H683 ◽  
Author(s):  
Lucas DeMaio ◽  
Mahsa Rouhanizadeh ◽  
Srinivasa Reddy ◽  
Alex Sevanian ◽  
Juliana Hwang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Endothelial Cells ◽  
Reactive Oxygen ◽  
Diffusive Flux ◽  
Oxygen Species ◽  
Aortic Endothelial Cells ◽  
Zonula Occludens ◽  
Bovine Aortic Endothelial Cells ◽  
Zonula Occludens 1 ◽  
Aortic Endothelial

Oxidized l-α-1-palmitoyl-2-arachidonoyl- sn-glycero-3-phosphorylcholine (OxPAPC), a component of minimally modified LDL, induces production of proinflammatory cytokines and development of atherosclerotic lesions. We tested the hypothesis that OxPAPC alters expression, phosphorylation, and localization of tight junction (TJ) proteins, particularly occludin, a transmembrane TJ protein. OxPAPC reduced total occludin protein and increased occludin phosphorylation dose dependently (10–50 μg/ml) and time dependently in bovine aortic endothelial cells. OxPAPC decreased occludin mRNA and reduced the immunoreactivity of zonula occludens-1 at the cell-cell contacts. Furthermore, OxPAPC increased the diffusive flux of 10-kDa dextran in a dose-dependent manner. O2−· production by bovine aortic endothelial cells increased nearly twofold after exposure to OxPAPC. Also, enzymatic generation of O2−· by xanthine oxidase-lumazine and H2O2 by glucose oxidase-glucose increased occludin phosphorylation, implicating reactive oxygen species as modulators of the OxPAPC effects on occludin phosphorylation. Superoxide dismutase and/or catalase blocked the effects of OxPAPC on occludin protein content and phosphorylation, occludin mRNA, zonula occludens-1 immunoreactivity, and diffusive flux of 10-kDa dextran. These findings suggest that changes in TJ proteins are potential mechanisms by which OxPAPC compromises the barrier properties of the vascular endothelium. OxPAPC-induced disruption of TJs, which likely facilitates transmigration of LDL and inflammatory cells into the subendothelial layers, may be mediated by reactive oxygen species.

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