The Effect of 3,5,3′-Triiodothyronine on Phosphoenolpyruvate Carboxykinase, Fatty Acid Synthetase and Malic Enzyme Activity of Liver and Brown Fat of Fetal and Neonatal Rats

Neonatology ◽  
1982 ◽  
Vol 41 (1-2) ◽  
pp. 1-7 ◽  
Author(s):  
Peter Hahn ◽  
Salim Hassanali
Keyword(s):  
Enzyme Activity ◽  
Fatty Acid ◽  
Malic Enzyme ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Fatty Acid Synthetase ◽  
Brown Fat ◽  
Neonatal Rats ◽  
Malic Enzyme Activity

Metabolic alterations after dehydroepiandrosterone treatment in Zucker rats

1984 ◽  
Vol 246 (2) ◽  
pp. E123-E128 ◽  
Author(s):  
A. Shepherd ◽  
M. P. Cleary
Keyword(s):  
Enzyme Activity ◽  
Adipose Tissue ◽  
Fatty Acid ◽  
Malic Enzyme ◽  
Fatty Acid Synthetase ◽  
Metabolic Effects ◽  
Zucker Rats ◽  
Obese Rats ◽  
Malic Enzyme Activity ◽  
Dhea Treatment

Dehydroepiandrosterone (DHEA) is a known noncompetitive inhibitor of glucose-6-phosphate dehydrogenase (G6PD). In the present investigation, the effects of chronic DHEA treatment on G6PD and several other enzymes involved in lipid metabolism were examined in lean and obese Zucker rats. Significant decreases in body weight were found in DHEA-treated rats in comparison with nontreated rats. In lean rats, DHEA treatment did not decrease either liver or adipose tissue G6PD and fatty acid synthetase activity, but malic enzyme activity was increased. In obese rats, decreased liver and adipose tissue G6PD and fatty acid synthetase activities were found. Malic enzyme activity in liver of obese DHEA rats was increased but not in adipose tissue. Adipose tissue lipoprotein lipase activity was decreased in both lean and obese DHEA rats. Serum insulin in obese DHEA rats was also decreased compared with control obese rats. These results indicate that the inhibition of G6PD may not be the mechanism of action of the antiobesity effect of DHEA. However, the metabolic effects of DHEA seen in obese rats may contribute to its antiobesity action.

The Effects of Catecholamines, Glucagon, and Diet on Enzyme Activities in Brown Fat and Liver of the Rat

1974 ◽  
Vol 52 (9) ◽  
pp. 739-743 ◽  
Author(s):  
Peter Hahn ◽  
Lorne T. Kirby
Keyword(s):  
Body Weight ◽  
Fatty Acid ◽  
Malic Enzyme ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Fatty Acid Synthetase ◽  
High Carbohydrate Diet ◽  
Brown Fat ◽  
Carbohydrate Diet ◽  
High Carbohydrate ◽  
Cleavage Enzyme

The effects of a high carbohydrate diet fed to young rats for 24–48 h on phosphoenolpyruvate carboxykinase, malic enzyme, citrate-cleavage enzyme, and fatty acid synthetase could be overcome by injecting the animals with glucagon (1 mg/100 g body weight) or norepinephrine (20 μg/100 g body weight) four times a day. The same effect was achieved with 50 mg ephedrine added to the 24 h diet. The catecholamines were more effective in brown fat, whereas glucagon seemed somewhat more effective in the liver.

The effect of starvation on obese mice

1981 ◽  
Vol 59 (4) ◽  
pp. 355-357 ◽  
Author(s):  
P. Hahn ◽  
J. P. Skala
Keyword(s):  
Fatty Acid ◽  
Plasma Levels ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Fatty Acid Synthetase ◽  
Brown Fat ◽  
Obese Group ◽  
Synthetase Activity ◽  
Obese Mice ◽  
Carnitine Acetyltransferase ◽  
Total Carnitine

Plasma levels of total and acylcarnitine and the activities of carnitine acetyltransferase (CAT) and carnitine, palmitoyltransferase (PCT) in liver and CAT in brown fat were determined in young obese (ob/ob) mice and their littermates during starvation. Plasma levels of acylcarnitine and β-hydroxybutyrate rose equally in both groups. Total carnitine levels, however, decreased in lean and rose in obese animals. Hepatic PCT and phosphoenolpyruvate carboxykinase activities rose more in lean than obese mice and brown fat CAT activity decreased in the obese group. Fatty acid synthetase activity decreased equally in the liver in obese mice and their lean littermates.

The effects of dietary fat on fatty acid synthesis and malic enzyme activity in liver from growing chicks

1969 ◽  
Vol 47 (7) ◽  
pp. 743-746 ◽  
Author(s):  
Alan G. Goodridge
Keyword(s):  
Fatty Acids ◽  
Enzyme Activity ◽  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Dietary Fat ◽  
Malic Enzyme ◽  
Corn Oil ◽  
Acid Synthesis ◽  
Hepatic Lipogenesis ◽  
Malic Enzyme Activity

Hepatic malic enzyme activity and incorporation of glucose-U-14C into fatty acids in liver slices was no greater in birds fed fat-free diets than in those fed the same diets supplemented with corn oil. The results suggest that in the chick, in contrast to the rat, dietary fat does not suppress hepatic lipogenesis.

Regulation of Phosphoenolpyruvate Carboxykinase and Fatty Acid Synthetase in Brown Fat of Suckling Rats*

Endocrinology ◽  
1978 ◽  
Vol 103 (4) ◽  
pp. 1417-1424 ◽  
Author(s):  
PETER HAHN ◽  
DAVID SECCOMBE ◽  
LORNE KIRBY ◽  
JEAN GIRARD ◽  
R. ASSAN ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Fatty Acid Synthetase ◽  
Brown Fat ◽  
Suckling Rats

Morphological and biochemical analyses of changes in rat hepatocytes related to wine and ethanol consumption

1984 ◽  
Vol 42 ◽  
pp. 232-233
Author(s):  
S.M. Geyer ◽  
C.L. Mendenhall ◽  
J.T. Hung ◽  
E.L. Cardell ◽  
R.L. Drake ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Enzyme Activity ◽  
Malic Enzyme ◽  
Smooth Endoplasmic Reticulum ◽  
Rat Hepatocytes ◽  
Mature Male ◽  
Treatment Groups ◽  
Malic Enzyme Activity ◽  
Biochemical Analyses

Thirty-three mature male Holtzman rats were randomly placed in 3 treatment groups: Controls (C); Ethanolics (E); and Wine drinkers (W). The animals were fed synthetic diets (Lieber type) with ethanol or wine substituted isocalorically for carbohydrates in the diet of E and W groups, respectively. W received a volume of wine which provided the same gram quantity of alcohol consumed by E. The animals were sacrificed by decapitation after 6 weeks and the livers processed for quantitative triglycerides (T3), proteins, malic enzyme activity (MEA), light microscopy (LM) and electron microscopy (EM). Morphometric analysis of randomly selected LM and EM micrographs was performed to determine organellar changes in centrilobular (CV) and periportal (PV) regions of the liver. This analysis (Table 1) showed that hepatocytes from E were larger than those in C and W groups. Smooth endoplasmic reticulum decreased in E and increased in W compared to C values.

‘Malic Enzyme’ Activity in Blowfly Muscle

Nature ◽  
1956 ◽  
Vol 177 (4514) ◽  
pp. 842-843 ◽  
Author(s):  
S. E. LEWIS ◽  
G. M. PRICE
Keyword(s):  
Enzyme Activity ◽  
Malic Enzyme ◽  
Malic Enzyme Activity
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