Phosphoramidon Modulates Effects of the 5-Lipoxygenase Inhibition on Anaphylactic Contraction of the Guinea Pig Trachea

1994 ◽  
Vol 103 (3) ◽  
pp. 293-297
Author(s):  
Neven Tudorić ◽  
Robert L. Coon ◽  
Zeljko J. Bosnjak
Keyword(s):  
Guinea Pig ◽  
Lipoxygenase Inhibition ◽  
Anaphylactic Contraction

Endogenous vasodilators modulate pulmonary vascular anaphylaxis

1994 ◽  
Vol 76 (2) ◽  
pp. 916-922 ◽  
Author(s):  
L. J. Kelly ◽  
B. J. Undem ◽  
G. K. Adams
Keyword(s):  
Nitric Oxide ◽  
Methylene Blue ◽  
Methyl Ester ◽  
Guinea Pig ◽  
Initial Phase ◽  
Contractile Response ◽  
Pulmonary Arteries ◽  
Relaxing Factor ◽  
Anaphylactic Contraction

We examined the role of endothelium-derived nitric oxide during antigen-induced contraction in pulmonary arteries isolated from actively sensitized guinea pigs. Ovalbumin (10(-2) mg/ml)-induced contraction was not sustained, and tension returned to baseline within 15 min. Pretreatment with methylene blue (10(-5) M) increased both the amplitude and the duration of the contractile response in these tissues. At 15 min, tension remained elevated and was > 70% of the peak amplitude. Removal of the endothelium with saponin (200 micrograms/ml) increased the magnitude of the contraction by > 125%; however, the duration of the response was unaffected. After pretreatment with saponin, methylene blue no longer increased the amplitude of antigen-induced contraction but its effect on the duration was unchanged. Pretreatment with nitro-L-arginine methyl ester significantly increased the magnitude of the contraction in each of the tissues. These results suggest that the response of guinea pig pulmonary arteries to antigen is modulated by two types of endogenous vasodilators, endothelium-derived nitric oxide that inhibits the initial phase of the response and an endothelium-independent relaxing factor that is guanosine 3′,5′-cyclic monophosphate dependent and attenuates the duration of anaphylactic contraction.

scholarly journals Quercetin Inhibits Anaphylactic Contraction of Guinea Pig Ileum Smooth Muscle

1983 ◽  
Vol 71 (4) ◽  
pp. 371-373 ◽  
Author(s):  
Michael J. Fanning ◽  
Peter Macander ◽  
Gary Drzewiecki ◽  
Elliott Middleton, Jr.
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Guinea Pig Ileum ◽  
Anaphylactic Contraction

Recovery after anaphylaxis of isolated guinea pig ileum segments

1961 ◽  
Vol 200 (5) ◽  
pp. 1083-1086 ◽  
Author(s):  
Sheldon E. Greisman
Keyword(s):  
Guinea Pig ◽  
Mechanism Of Action ◽  
Muscle Tone ◽  
Active Participation ◽  
Guinea Pig Ileum ◽  
Relaxation Phase ◽  
Impaired Ability ◽  
Contractile System ◽  
Spontaneous Relaxation ◽  
Anaphylactic Contraction

Following anaphylactic contraction of actively sensitized, isolated guinea pig ileum segments in a continuously aerated system, spontaneous relaxation ensues. Interruption of aeration during anaphylactic contraction consistently inhibited the relaxation phase. If aeration was resumed, relaxation promptly ensued. Such relaxation was not an all-or-none reaction, but could be made to occur in stepwise fashion by intermittent interruption of aeration. These effects of interruption of aeration on muscle tone during anaphylaxis appeared specific, since opposite tone changes were observed during contractions induced with exogenous histamine. Studies with Tyrode's solution, pretreated with 100% O2 or with 98.5% O2–1.5% CO2, suggested that the mechanism of action of aeration was, in part at least, mediated by an oxygen-dependent process and implicated the active participation of intracellular histamine-inactivating systems. Although postanaphylactic ileum relaxation was also accelerated in Tyrode's solution pretreated with 100% N2, this effect differed in important details from accelerated relaxation in oxygenated systems and appeared to depend upon impaired ability of the contractile system to respond to released histamine.

Effects of 5-Lipoxygenase Inhibition on Cardiac Anaphylaxis in Isolated Guinea Pig Hearts

Pharmacology ◽  
1989 ◽  
Vol 38 (6) ◽  
pp. 352-362 ◽  
Author(s):  
S.R. Jolly ◽  
J. Travis ◽  
R.G. Van Inwegen
Keyword(s):  
Guinea Pig ◽  
Cardiac Anaphylaxis ◽  
Lipoxygenase Inhibition

Role of histamine or other released substances in anaphylaxis in isolated guinea pig ileum

1959 ◽  
Vol 197 (1) ◽  
pp. 31-33 ◽  
Author(s):  
Murray Dworetzky
Keyword(s):  
Guinea Pig ◽  
Anaphylactic Reaction ◽  
Active Substance ◽  
Guinea Pig Ileum ◽  
Anaphylactic Contraction

A study was made of the role of histamine or other active liberated substances in anaphylaxis in isolated segments of guinea pig ileum. A technique was used by which it can be quantitatively estimated how much of the total anaphylactic reaction is due to liberation of such substances. The method consisted of suspending a segment from a sensitized animal in a 40-ml bath, together with a segment called the ‘indicator segment’, from a normal, nonsensitized animal. Thus, during anaphylactic contraction of the sensitized segment, histamine or any other active substance liberated into the bath would be expected to diffuse through the perfusion bath and cause a contraction of the so-called ‘indicator segment’. The height of the contractions was roughly estimated in terms of that amount of histamine necessary to cause a contraction of the same intensity. Using this technique, it was concluded that no more than 10% of the total anaphylactic contraction in the isolated sensitized guinea pig ileum can be accounted for by histamine or any other substance liberated into the bath fluid.

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