scholarly journals Pharmacological characterization of the in vitro anaphylactic contraction of the guinea-pig esophageal muscularis mucosae.

1988 ◽  
Vol 91 (1) ◽  
pp. 17-27 ◽  
Author(s):  
Sachie FUJINUMA
Keyword(s):  
Guinea Pig ◽  
Muscularis Mucosae ◽  
Pharmacological Characterization ◽  
Anaphylactic Contraction

Morphological and Pharmacological Characterization of Guinea-Pig Prostatic Smooth Muscle Cells in Vitro

1997 ◽  
Vol 4 (2) ◽  
pp. 186-190 ◽  
Author(s):  
Naohito Mikuma ◽  
Seiji Furuya ◽  
Hiroshi Isomura ◽  
Hideo Yabu ◽  
Norimasa Sawada ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Pharmacological Characterization

Prejunctional inhibitory muscarinic receptors on cholinergic nerves in human and guinea pig airways

1988 ◽  
Vol 64 (6) ◽  
pp. 2532-2537 ◽  
Author(s):  
P. A. Minette ◽  
P. J. Barnes
Keyword(s):  
Guinea Pig ◽  
Muscarinic Receptors ◽  
Contractile Response ◽  
Electrical Field Stimulation ◽  
Cholinergic Nerves ◽  
Ach Release ◽  
Pharmacological Characterization ◽  
Human Airways

We have investigated whether prejunctional inhibitory muscarinic receptors ("autoreceptors") exist on cholinergic nerves in human airways in vitro and whether guinea pig trachea provides a good model for further pharmacological characterization of these receptors. Pilocarpine was used as a selective agonist and gallamine as a selective antagonist of these autoreceptors. Acetylcholine (ACh) release from postganglionic cholinergic nerves was elicited by electrical field stimulation (EFS) (40 V, 0.5 ms, 32 Hz). In human bronchi, pilocarpine inhibited the contractile response to EFS in a dose-related fashion; the dose inhibiting 50% of the control contraction was 2.2 +/- 0.4 x 10(-7) (SE) M (n = 22), and the inhibition was 96% at 3 x 10(-5) M. The inhibitory effects of pilocarpine were antagonized by gallamine in a dose-related fashion. The results were qualitatively the same in the guinea pig. Gallamine significantly enhanced the contractile response to EFS in the guinea pig, whereas pirenzepine failed to do so, which suggests that M2-receptors are involved. We conclude that prejunctional muscarinic receptors that inhibit ACh release are present on cholinergic nerves in human airways and that guinea pig trachea is a good model for further pharmacological characterization of these receptors, which appear to belong to the M2-subtype.

Pharmacological characterization of opossum distal colonic muscularis mucosae in vitro

1986 ◽  
Vol 250 (1) ◽  
pp. G98-G102 ◽  
Author(s):  
W. H. Percy ◽  
J. Christensen
Keyword(s):  
Electrical Field Stimulation ◽  
Distal Colon ◽  
Field Stimulation ◽  
Muscularis Mucosae ◽  
Electrical Field ◽  
Pharmacological Characterization ◽  
Neural Inhibition ◽  
Spontaneous Contractions

In muscularis mucosae from the opossum distal colon, both tone and spontaneous contractions were highly dependent on the available oxygen. Acetylcholine and histamine caused, respectively, atropine- and pyrilamine-sensitive contractions. Norepinephrine relaxed the tissue, an effect abolished by propranolol. Under these conditions norepinephrine failed to elicit contractions and at higher concentrations again caused relaxations. The tissue gave concentration-dependent relaxations to ATP but not to ADP, AMP, or adenosine. Electrical field stimulation (20-30 Hz, 1-2 ms, 120 mA) revealed a cholinergic excitatory innervation and a nonadrenergic, noncholinergic neural inhibition. Cholecystokinin, gastrin, substance P, and vasoactive intestinal polypeptide were without effect on this tissue. In these respects, colonic muscularis mucosae differs considerably from that of other gastrointestinal viscera.

Sign in / Sign up

Export Citation Format

Share Document