Effect of Glycoprotein-Processing Inhibitors on the Mouse IgE Binding Capacity of Rat Basophilic Leukemia Cells

1987 ◽  
Vol 84 (1) ◽  
pp. 85-92 ◽  
Author(s):  
Claire Gavériaux ◽  
Francis Loor
Keyword(s):  
Binding Capacity ◽  
Leukemia Cells ◽  
Ige Binding ◽  
Rat Basophilic Leukemia Cells ◽  
Glycoprotein Processing ◽  
Basophilic Leukemia

Protein Kinase C Activators of the Teleocidin Family Decrease the IgE-Binding Capacity of Rat Basophilic Leukemia Cells

1988 ◽  
Vol 86 (4) ◽  
pp. 465-471 ◽  
Author(s):  
Claire Gavériaux ◽  
Theodor Fehr ◽  
Encarnita Montecino-Rodriguez ◽  
Jean-Jacques Sanglier ◽  
Francis Loor
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Binding Capacity ◽  
Leukemia Cells ◽  
Kinase C ◽  
Ige Binding ◽  
Protein Kinase C Activators ◽  
Rat Basophilic Leukemia Cells ◽  
Basophilic Leukemia

scholarly journals THE INTERACTION OF IgE WITH RAT BASOPHILIC LEUKEMIA CELLS

1974 ◽  
Vol 140 (6) ◽  
pp. 1676-1695 ◽  
Author(s):  
Anthony Kulczycki ◽  
Henry Metzger
Keyword(s):  
Binding Capacity ◽  
High Specificity ◽  
Leukemia Cells ◽  
Binding Reaction ◽  
Reversible Binding ◽  
Surface Receptors ◽  
Rat Basophilic Leukemia Cells ◽  
Number Of Binding Sites ◽  
Complete Dissociation ◽  
Basophilic Leukemia

Previous studies indicated that cultured rat basophilic leukemia cells have surface receptors which bind IgE with high specificity. In this paper we describe some quantitative aspects of the phenomenon. The reaction mechanism appears to consist of a simple reversible binding reaction with k1 = 9.6 x 104 M–1 sec–1 and k–1 ⩽ 1.6 x 10–5 sec–1 at 37°C. The calculated KA is therefore ⩾ 6 x 109 M–1. The activation energy of binding was found to be 7.8 kcal/mol. The number of binding sites/cell varied between 3 x 105 to over 1 x 106. The binding was insensitive to pH's between 6–8 but at pH 3.0 complete dissociation of bound IgE occurred in ∼ 1 min at 0°C leaving the receptors for IgE intact. Ca++ plus EDTA and Mg++ plus EDTA produce a fairly marked reduction in binding capacity though these reagents alone produce much smaller effects.

The distribution of antigen on the surface of RBL-2H3 rat basophilic leukemia cells observed by back-scattered electron imaging

1986 ◽  
Vol 44 ◽  
pp. 274-275
Author(s):  
R.F. Stump ◽  
J.R. Pfeiffer ◽  
JC. Seagrave ◽  
D. Huskisson ◽  
J.M. Oliver
Keyword(s):  
Cell Surface ◽  
Dynamic Properties ◽  
Leukemia Cells ◽  
Antigen Binding ◽  
Scattered Electron ◽  
Ige Receptor ◽  
Receptor Complexes ◽  
Rat Basophilic Leukemia Cells ◽  
Electron Imaging ◽  
Basophilic Leukemia

In RBL-2H3 rat basophilic leukemia cells, antigen binding to cell surface IgE-receptor complexes stimulates the release of inflammatory mediators and initiates a series of membrane and cytoskeletal events including a transformation of the cell surface from a microvillous to a lamellar topography. It is likely that dynamic properties of the IgE receptor contribute to the activation of these responses. Fewtrell and Metzger have established that limited crosslinking of IgE-receptor complexes is essential to trigger secretion. In addition, Baird and colleagues have reported that antigen binding causes a rapid immobilization of IgE-receptor complexes, and we have demonstrated an apparent increase with time in the affinity of IgE-receptor complexes for antigen.

Immunoglobulin E mediated membrane conductance changes in rat basophilic leukemia cells

1988 ◽  
Vol 66 (3) ◽  
pp. 328-331 ◽  
Author(s):  
Carlos Barajas-López ◽  
Jan D. Huizinga
Keyword(s):  
Immunoglobulin E ◽  
Calcium Influx ◽  
Membrane Conductance ◽  
Membrane Resistance ◽  
Leukemia Cells ◽  
Rat Basophilic Leukemia Cells ◽  
Conductance Changes ◽  
Electrophysiological Effects ◽  
Basophilic Leukemia ◽  
Stimulation Of

Electrophysiological effects of anaphylactic stimulation of rat basophilic leukemia cells (RBL-2H3) were studied using conventional microelectrodes. Stimulation of passively sensitized cells by anti-immunoglobulin E resulted in hyperpolarization followed by depolarization. These changes in membrane polarization were associated with a decrease in input membrane resistance. No effect of anaphylactic stimulation was seen in Ca2+-free solution or when Ca2+ influx was blocked by Co2+, but it was mimicked by the Ca2+ ionophore A-23187. This suggests that the changes in ionic conductances were associated with calcium influx. These results support the hypothesis that membrane conductance changes are involved in the stimulus-secretion process of the RBL-2H3 cells.

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