Immunoglobulin E mediated membrane conductance changes in rat basophilic leukemia cells

1988 ◽  
Vol 66 (3) ◽  
pp. 328-331 ◽  
Author(s):  
Carlos Barajas-López ◽  
Jan D. Huizinga
Keyword(s):  
Immunoglobulin E ◽  
Calcium Influx ◽  
Membrane Conductance ◽  
Membrane Resistance ◽  
Leukemia Cells ◽  
Rat Basophilic Leukemia Cells ◽  
Conductance Changes ◽  
Electrophysiological Effects ◽  
Basophilic Leukemia ◽  
Stimulation Of

Electrophysiological effects of anaphylactic stimulation of rat basophilic leukemia cells (RBL-2H3) were studied using conventional microelectrodes. Stimulation of passively sensitized cells by anti-immunoglobulin E resulted in hyperpolarization followed by depolarization. These changes in membrane polarization were associated with a decrease in input membrane resistance. No effect of anaphylactic stimulation was seen in Ca2+-free solution or when Ca2+ influx was blocked by Co2+, but it was mimicked by the Ca2+ ionophore A-23187. This suggests that the changes in ionic conductances were associated with calcium influx. These results support the hypothesis that membrane conductance changes are involved in the stimulus-secretion process of the RBL-2H3 cells.

Changes in the receptor for immunoglobulin E coincident with receptor-mediated stimulation of basophilic leukemia cells

Biochemistry ◽  
1983 ◽  
Vol 22 (25) ◽  
pp. 5733-5737 ◽  
Author(s):  
Ruy Perez-Monfort ◽  
Clare Fewtrell ◽  
Henry Metzger
Keyword(s):  
Immunoglobulin E ◽  
Leukemia Cells ◽  
Basophilic Leukemia ◽  
Stimulation Of

scholarly journals Small oligomers of immunoglobulin E (IgE) cause large-scale clustering of IgE receptors on the surface of rat basophilic leukemia cells.

1984 ◽  
Vol 98 (2) ◽  
pp. 577-583 ◽  
Author(s):  
A K Menon ◽  
D Holowka ◽  
B Baird
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Surface ◽  
Large Scale ◽  
Immunoglobulin E ◽  
Leukemia Cells ◽  
Ige Receptors ◽  
Rat Basophilic Leukemia Cells ◽  
A Cell ◽  
Basophilic Leukemia

We examined the distribution of small oligomers of IgE bound to rat basophilic leukemia cells using fluorescence microscopy. The oligomers were seen to cluster into visible patches on the cell surface at 4 degrees C; at higher temperatures internalization also was observed. In contrast, cells labeled with IgE monomers remained predominantly ring-stained. Evidence is provided that the observed clustering of IgE oligomers is a cell-induced phenomenon, and the possible significance of this clustering is discussed in the context of the oligomer-triggered degranulation of rat basophilic leukemia cells.

Influence of the antiallergic drug oxatomide and derivatives on membrane structures: relation with inhibition of calcium influx in rat basophilic leukemia cells

1999 ◽  
Vol 57 (5) ◽  
pp. 503-510 ◽  
Author(s):  
Jeannette J.C. Paulussen ◽  
Marcel J.E. Fischer ◽  
Nicolaas J. Zuidam ◽  
J.Cees v. Miltenburg ◽  
Nico J. de Mol ◽  
...  
Keyword(s):  
Calcium Influx ◽  
Leukemia Cells ◽  
Membrane Structures ◽  
Rat Basophilic Leukemia Cells ◽  
Antiallergic Drug ◽  
Basophilic Leukemia

scholarly journals Clustering, mobility, and triggering activity of small oligomers of immunoglobulin E on rat basophilic leukemia cells.

1986 ◽  
Vol 102 (2) ◽  
pp. 534-540 ◽  
Author(s):  
A K Menon ◽  
D Holowka ◽  
W W Webb ◽  
B Baird
Keyword(s):  
Large Scale ◽  
Immunoglobulin E ◽  
Gel Filtration ◽  
Leukemia Cells ◽  
Fluorescence Photobleaching Recovery ◽  
Photobleaching Recovery ◽  
Rat Basophilic Leukemia Cells ◽  
Cellular Components ◽  
Basophilic Leukemia ◽  
Selective Interactions

We have recently shown that small oligomers of IgE bound to univalent receptors for IgE on the surface of rat basophilic leukemia cells induce extensive aggregation of the receptors at 4 degrees C into patches resolvable by fluorescence microscopy and that this does not occur with monomeric IgE (Menon, A. K., D. Holowka, and B. Baird, 1984, J. Cell Biol. 98:577-583). Here we use fluorescence photobleaching recovery measurements to show that receptor oligomerization by this means is accompanied by a dramatic reduction of receptor lateral mobility, and that this immobilization occurs even when the clustering is not microscopically detectable. Furthermore, the degree of immobility induced by a particular oligomer fraction from a gel filtration column correlates positively with its ability to trigger cellular degranulation, whereas receptors labeled with monomeric IgE have no triggering activity and exhibit typical membrane protein mobility. The slow, large-scale oligomer-induced clustering appears to be a long term consequence of earlier selective interactions that result in receptor immobilization, and this highly clustered state provides a competent, noninhibitory triggering signal resulting in cellular degranulation upon warming to 37 degrees C. We conclude that even limited clustering of IgE receptors on rat basophilic leukemia cells induces interactions with other cellular components that constrain receptor mobility and eventually cause massive coalescence of the clusters. These primary selective interactions occurring at the level of receptor oligomers or small clusters of oligomers that result in immobilization may play a role in triggering cellular degranulation.

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