Protein Kinase C Activators of the Teleocidin Family Decrease the IgE-Binding Capacity of Rat Basophilic Leukemia Cells

1988 ◽  
Vol 86 (4) ◽  
pp. 465-471 ◽  
Author(s):  
Claire Gavériaux ◽  
Theodor Fehr ◽  
Encarnita Montecino-Rodriguez ◽  
Jean-Jacques Sanglier ◽  
Francis Loor
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Binding Capacity ◽  
Leukemia Cells ◽  
Kinase C ◽  
Ige Binding ◽  
Protein Kinase C Activators ◽  
Rat Basophilic Leukemia Cells ◽  
Basophilic Leukemia

scholarly journals Effects of Inhibitors of Protein Kinase C on the Release and Synthesis of Histamine in Rat Basophilic Leukemia Cells (2H3).

1992 ◽  
Vol 58 (3) ◽  
pp. 291-298 ◽  
Author(s):  
Kazutaka Maeyama ◽  
Yoshitaka Taguchi ◽  
Eiko Sakurai ◽  
Masayuki Sasaki ◽  
Atsushi Yamatodani ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Leukemia Cells ◽  
Kinase C ◽  
Rat Basophilic Leukemia Cells ◽  
Basophilic Leukemia

Rat Basophilic Leukemia Cells: Protein Kinase C and Secretion

1991 ◽  
Vol 20 (4) ◽  
pp. 407-420 ◽  
Author(s):  
P. André ◽  
A. M. Benoliel ◽  
M. Soler ◽  
D. Vervloet ◽  
P. Bongrand ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Leukemia Cells ◽  
Kinase C ◽  
Rat Basophilic Leukemia Cells ◽  
Basophilic Leukemia

scholarly journals Effects of Inhibitors of Protein Kinase C on the Release and Synthesis of Histamine in Rat Basophilic Leukemia Cells (2H3)

1992 ◽  
Vol 58 (3) ◽  
pp. 291-298
Author(s):  
Kazutaka Maeyama ◽  
Yoshitaka Taguchi ◽  
Eiko Sakurai ◽  
Masayuki Sasaki ◽  
Atsushi Yamatodani ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Leukemia Cells ◽  
Kinase C ◽  
Rat Basophilic Leukemia Cells ◽  
Basophilic Leukemia

scholarly journals Activation of protein kinase C in rat basophilic leukemia cells stimulates increased production of phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate: correlation with actin polymerization.

1995 ◽  
Vol 6 (1) ◽  
pp. 97-108 ◽  
Author(s):  
J R Apgar
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Actin Polymerization ◽  
Immunoglobulin E ◽  
Actin Binding ◽  
Kinase C ◽  
Rat Basophilic Leukemia Cells ◽  
Rbl Cells ◽  
Phosphatidylinositol 4 ◽  
Basophilic Leukemia

Cross-linking of the immunoglobulin E receptor on rat basophilic leukemia (RBL)1 cells by multivalent antigen activates phosphatidylinositol (PI) kinase and phosphatidylinositol 4-phosphate (PIP) kinase leading to the increased production of PIP and phosphatidylinositol 4,5-bisphosphate (PIP2). Activators of protein kinase C (PKC), such as phorbol myristate acetate (PMA) and the synthetic diacylglycerol, 1,2-dioctanoyl-sn-glycerol (diC8), were found to have the same effect even though PMA and diC8 do not cause the activation of phospholipase C. Although the kinetics are different depending on the stimulant, activation of PKC using multivalent antigen, PMA or diC8 also causes the polymerization of actin and an increase in the F-actin content of the cells. In all cases, a good correlation was observed between F-actin levels, activation of PI and PIP kinases, and the increased production of PIP and PIP2. However, in the case of antigen, there is no correlation between actin polymerization and the total amount of PIP and PIP2. Staurosporine, an inhibitor of protein kinases, blocks the F-actin response and the increased synthesis of PIP and PIP2 with similar dose dependencies. Furthermore, depletion of PKC activity through long-term exposure to PMA, inhibited both the F-actin response and the increased synthesis of PIP and PIP2 induced by either DNP-BSA or diC8. These results suggest that activation of PKC precedes the activation of PI and PIP kinases and that under certain circumstances activation of the kinases and the increased synthesis of PIP and PIP2 may be involved in the polymerization of actin in RBL cells, possibly through the interaction of the polyphosphoinositides with actin-binding proteins such as gelsolin and profilin.

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