Immunogenicity of p-Phenetidine, 2-Hydroxy-p-Phenetidine and their Protein Conjugates in Guinea Pigs, Rabbits and Man

1977 ◽  
Vol 53 (4) ◽  
pp. 319-328 ◽  
Author(s):  
P.E. Erne ◽  
A.F. Geczy ◽  
H. Spengler ◽  
A.L. de Weck ◽  
U.C. Dubach
Keyword(s):  
Guinea Pigs ◽  
Protein Conjugates

scholarly journals STUDIES ON THE SENSITIZATION OF ANIMALS WITH SIMPLE CHEMICAL COMPOUNDS

1937 ◽  
Vol 66 (3) ◽  
pp. 337-351 ◽  
Author(s):  
K. Landsteiner ◽  
M. W. Chase
Keyword(s):  
Contact Dermatitis ◽  
Strong Evidence ◽  
Guinea Pigs ◽  
Chemical Properties ◽  
Chemical Compounds ◽  
Protein Conjugates ◽  
Chemical Constitution ◽  
Simple Chemical ◽  
Picryl Chloride ◽  
Cutaneous Administration

It has been shown that by the cutaneous administration of simple chemical compounds in small quantities—2:4:6 trinitrochlorobenzene (picryl chloride) and 2:4 dinitrochlorobenzene, the latter a typical incitant of contact dermatitis in man—it is possible to induce true anaphylactic sensitization in guinea pigs, demonstrable by the intravenous injection of protein conjugates and by the Dale technique, using isolated uterine horns. This furnishes strong evidence for the formation of antigenic conjugates following application of substances of simple chemical constitution. Since the anaphylactic state is induced by the same method of administration that gives rise to cutaneous sensitivity, the assumption would appear justified, when one takes into account the chemical properties of the inciting substances, that the formation of conjugated antigens offers an explanation for the skin effects also. In the experiments with picryl chloride, anaphylactic antibodies, and occasionally precipitins, have been demonstrated. The differences between the cutaneous and anaphylactic types of sensitivity are discussed.

RAPID PURIFICATION OF TRI-IODOTHYRONINE AND THYROXINE PROTEIN CONJUGATES FOR ANTIBODY PRODUCTION

1975 ◽  
Vol 65 (1) ◽  
pp. 133-138 ◽  
Author(s):  
C. W. BURKE ◽  
R. A. SHAKESPEAR
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Antibody Production ◽  
Guinea Pigs ◽  
High Titre ◽  
Adsorption Chromatography ◽  
Protein Conjugates ◽  
Rapid Purification ◽  
Dialysis Technique

SUMMARY Thyroxine (T4) and tri-iodothyronine (T3) were coupled to human serum albumin (HSA) with carbodi-imide. By adsorption chromatography on Sephadex G-25, fractions containing purified conjugate, but not reversibly-bound T3 or T4, were obtained, and this procedure took 5 h; considerably less than the conventional dialysis technique. Highly specific high-titre antisera were produced in rabbits and guinea-pigs by injection of these fractions in Freund's adjuvant.

scholarly journals STUDIES ON THE SENSITIZATION OF ANIMALS WITH SIMPLE CHEMICAL COMPOUNDS

1947 ◽  
Vol 86 (6) ◽  
pp. 489-514 ◽  
Author(s):  
Merrill W. Chase
Keyword(s):  
Guinea Pigs ◽  
Chemical Compounds ◽  
Human Beings ◽  
Acyl Chlorides ◽  
Protein Conjugates ◽  
Simple Chemical ◽  
Picryl Chloride ◽  
Acid Anhydrides ◽  
Substantial Degree

Evidence is presented to show that guinea pigs actively sensitized to simple chemical compounds form serum antibodies capable of sensitizing the skin of normal guinea pigs. Skin sites prepared as for the Prausnitz-Küstner test develop immediate-type ("evanescent") reactions with erythema and edema, upon subsequent injection of the corresponding simple compounds or protein conjugates thereof, and give effects resembling transferred reaginic reactions as seen in human beings. The antibodies were obtainable after sensitization by acyl chlorides, acid anhydrides, and also substances of lesser reactivity, picryl chloride and 2:4 dinitrochlorobenzene, which are human allergens. Observations are reported on the specificity of the antibodies and on various details of the reaction. Like effects result when antiprotein immune sera and their corresponding antigens are employed for the test, making it highly probable that the antibodies secured after sensitization to drugs result from immunization by conjugates formed in vivo. The sera obtained after sensitization with simple chemical compounds readily confer passive anaphylaxis, and their capacity for sensitizing the skin declines gradually with progressive heating. It was observed that following a reaction of substantial degree in guinea pig skin the area involved does not fully recover for some days its capacity to react, the effect being a manifestation, it would seem, of what has been termed "non-specific antianaphylaxis."

scholarly journals CARRIER FUNCTION IN ANTI-HAPTEN IMMUNE RESPONSES

1970 ◽  
Vol 132 (2) ◽  
pp. 283-299 ◽  
Author(s):  
William E. Paul ◽  
David H. Katz ◽  
Edmond A. Goidl ◽  
Baruj Benacerraf
Keyword(s):  
Immune Responses ◽  
Guinea Pigs ◽  
Preceding Paper ◽  
Lymphoid Cells ◽  
Antibody Responses ◽  
Cooperative Interaction ◽  
Antigenic Determinants ◽  
Cell Transfer ◽  
Enhancing Effect ◽  
Protein Conjugates

Transfer of live lymphoid cells from BGG-immunized strain 2 guinea pigs into syngeneic animals primed with DNP-OVA prepares the recipients for a markedly enhanced secondary anti-DNP antibody response upon challenge with DNP-BGG. This phenomenon has been demonstrated when the recipients were challenged 1 day after cell transfer, but it was considerably more striking when an interval of 6 days was allowed between transfer of cells and challenge with antigen. As demonstrated in the preceding paper (6), BGG preimmunization enhanced anti-DNP antibody responses to challenge with DNP-BGG. An analysis of the characteristics of substances to which preimmunization was effective in enhancing subsequent anti-hapten responses was made. It was shown that preimmunization of strain 13 guinea pigs with a copolymer of glutamic acid and lysine (GL), to which these animals are genetically unable to accord an immune response, failed to prepare them for an enhanced anti-DNP response to DNP-GL. Tolerance to BGG specifically abrogated the capacity of subsequent BGG immunization to prepare DNP-OVA primed rabbits for an enhanced anti-DNP response to DNP-BGG. Sensitization of animals to haptens by preimmunization with hapten-protein conjugates failed to prepare them for enhanced primary or secondary antibody responses to other determinants associated with that hapten on a different carrier. These studies indicate that the enhancing effect of carrier preimmunization reflects a cooperative interaction between lymphoid cells specific for carrier and cells specialized for haptenic determinants. Furthermore, the capacity of a substance to behave as a carrier requires more than its possession of a variety of antigenic determinants.

Induction of Photoallergy in Guinea-Pigs by Injection of Photoallergen-Protein Conjugates

1987 ◽  
Vol 84 (4) ◽  
pp. 385-389 ◽  
Author(s):  
M.D. Barratt ◽  
B.F.J. Goodwin ◽  
W.W. Lovell
Keyword(s):  
Guinea Pigs ◽  
Protein Conjugates

Investigation of the relaxant effects of propofol on ovalbumin-induced asthma in guinea pigs

2006 ◽  
pp. 1
Author(s):  
I. Bagcivan ◽  
O. Cevit ◽  
M. K. Yildirim ◽  
S. Gursoy ◽  
S. Yildirim ◽  
...  
Keyword(s):  
Guinea Pigs
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