Development of Contact Sensitivity to DNFB in Guinea Pigs Genetically Differing in Their Response to DNP Skin Protein Conjugates

1974 ◽  
Vol 46 (3) ◽  
pp. 417-426 ◽  
Author(s):  
L. Polak ◽  
Annemarie Polak-Wyss ◽  
J.R. Frey
Keyword(s):  
Guinea Pigs ◽  
Contact Sensitivity ◽  
Protein Conjugates ◽  
Skin Protein

Contact Sensitivity and Immunologic Tolerance in Germfree Guinea Pigs

1972 ◽  
Vol 141 (2) ◽  
pp. 522-526 ◽  
Author(s):  
M. H. Friedlaender ◽  
H. Baer ◽  
P. R. B. McMaster
Keyword(s):  
Guinea Pigs ◽  
Immunologic Tolerance ◽  
Contact Sensitivity

scholarly journals The Inability of Certain Fluorochromes to Induce Contact Sensitivity in Guinea Pigs

1964 ◽  
Vol 42 (4) ◽  
pp. 279-280 ◽  
Author(s):  
Florante C. Bocobo ◽  
Phyllis D. Kind ◽  
Arthur C. Curtis
Keyword(s):  
Guinea Pigs ◽  
Contact Sensitivity

Immunogenicity of p-Phenetidine, 2-Hydroxy-p-Phenetidine and their Protein Conjugates in Guinea Pigs, Rabbits and Man

1977 ◽  
Vol 53 (4) ◽  
pp. 319-328 ◽  
Author(s):  
P.E. Erne ◽  
A.F. Geczy ◽  
H. Spengler ◽  
A.L. de Weck ◽  
U.C. Dubach
Keyword(s):  
Guinea Pigs ◽  
Protein Conjugates

scholarly journals STUDIES ON THE SENSITIZATION OF ANIMALS WITH SIMPLE CHEMICAL COMPOUNDS

1937 ◽  
Vol 66 (3) ◽  
pp. 337-351 ◽  
Author(s):  
K. Landsteiner ◽  
M. W. Chase
Keyword(s):  
Contact Dermatitis ◽  
Strong Evidence ◽  
Guinea Pigs ◽  
Chemical Properties ◽  
Chemical Compounds ◽  
Protein Conjugates ◽  
Chemical Constitution ◽  
Simple Chemical ◽  
Picryl Chloride ◽  
Cutaneous Administration

It has been shown that by the cutaneous administration of simple chemical compounds in small quantities—2:4:6 trinitrochlorobenzene (picryl chloride) and 2:4 dinitrochlorobenzene, the latter a typical incitant of contact dermatitis in man—it is possible to induce true anaphylactic sensitization in guinea pigs, demonstrable by the intravenous injection of protein conjugates and by the Dale technique, using isolated uterine horns. This furnishes strong evidence for the formation of antigenic conjugates following application of substances of simple chemical constitution. Since the anaphylactic state is induced by the same method of administration that gives rise to cutaneous sensitivity, the assumption would appear justified, when one takes into account the chemical properties of the inciting substances, that the formation of conjugated antigens offers an explanation for the skin effects also. In the experiments with picryl chloride, anaphylactic antibodies, and occasionally precipitins, have been demonstrated. The differences between the cutaneous and anaphylactic types of sensitivity are discussed.

scholarly journals STUDIES OF HYPERSENSITIVITY TO LOW MOLECULAR WEIGHT SUBSTANCES

1953 ◽  
Vol 98 (6) ◽  
pp. 533-549 ◽  
Author(s):  
Herman N. Eisen ◽  
Sidney Belman
Keyword(s):  
Skin Surface ◽  
Chromatographic Technique ◽  
Human Beings ◽  
Amino Groups ◽  
Sh Groups ◽  
Protein Conjugates ◽  
Ph Values ◽  
Skin Protein

2,4-dinitrophenylsulfenyl chloride (DSCl) and 2,4-dinitrophenylthiocyanate (DSCN) elicited allergic reactions of the delayed type when applied to the skin of guinea pigs and of human beings who had been sensitized by prior exposure to 2,4-dinitrofluorobenzene (DF). DSCl and DSCN, together with 2,4-dinitrobenzene sulfonate (DSO3), constitute a clearly defined group of allergenic dinitrophenyl compounds in that they all combined with skin protein in vivo through reaction with cysteine or cystine. In vitro, these compounds combine with free SH groups, and with —S—S— groups of hair and epidermis, but not with —S—S— groups of oxidized glutathione or of bovine gamma globulins. DSO3, DSCl, and DSCN did not react with amino groups in vivo, but did react with protein amino groups in vitro at pH values of about 10. Another group of dinitrophenyl compounds (DF, DCl, and DBr) previously had been shown to combine with lysine ϵ-NH2 groups of epidermal proteins. In the present work it was found that these compounds do not react with the disulfide groups of these proteins, either in vivo or in vitro. Moreover, they did not seem to react with SH groups of viable skin, although they are highly reactive with sulfhydryl in vitro. This apparent discrepancy between reactivity with SH groups in vitro and in vivo may be due to the fact that the chromatographic technique employed was relatively insensitive for the sulfhydryl derivative. When a compound of either group was applied to the skin surface, dinitrophenyl-amino acids were recovered from the epidermis but not from the dermis. The results are discussed from the viewpoint of the epidermal localization of dinitrophenyl-protein conjugates.

scholarly journals ELICITATION OF DELAYED ALLERGIC SKIN REACTIONS WITH HAPTENS: THE DEPENDENCE OF ELICITATION ON HAPTEN COMBINATION WITH PROTEIN

1952 ◽  
Vol 95 (5) ◽  
pp. 473-487 ◽  
Author(s):  
Herman N. Eisen ◽  
Leo Orris ◽  
Sidney Belman
Keyword(s):  
Guinea Pigs ◽  
Necessary Condition ◽  
Previous Exposure ◽  
Skin Reactions ◽  
Skin Protein ◽  
Allergic Skin ◽  
High Degree

Eight 2,4-dinitrophenyl compounds with a high degree of configurational uniformity were examined in regard to their ability to elicit delayed allergic skin reactions in guinea pigs and men who had been sensitized by previous exposure to 2,4-dinitrofluorobenzene. Four of the eight compounds produced reactions (elicitors) and four others did not (non-elicitors). It was found that the elicitors combined with protein in vitro and in vivo. The non-elicitors, however, failed to combine in either situation. It is concluded that a necessary condition for the elicitation of delayed allergic skin reactions by haptens is the combination of the latter with skin protein through the formation of bonds of the covalent type. No choice is yet possible amongst the several possible explanations which are advanced to account for the obligatory character of protein combination.

Sign in / Sign up

Export Citation Format

Share Document