A Unique Rapid Enhancement by Adjuvant of Delayed Hypersensitivity to Protein Antigens in the Guinea Pig

1970 ◽  
Vol 38 (4) ◽  
pp. 427-441 ◽  
Author(s):  
H.C. Maguire, Jr.
Keyword(s):  
Guinea Pig ◽  
Delayed Hypersensitivity ◽  
Protein Antigens

scholarly journals DELAYED HYPERSENSITIVITY

1957 ◽  
Vol 105 (1) ◽  
pp. 11-24 ◽  
Author(s):  
Jonathan W. Uhr ◽  
S. B. Salvin ◽  
A. M. Pappenheimer
Keyword(s):  
Guinea Pig ◽  
Guinea Pigs ◽  
Intradermal Injection ◽  
Delayed Hypersensitivity ◽  
Protein Antigens ◽  
Single Protein ◽  
Maximal Sensitivity ◽  
Degree Of Sensitization ◽  
Circulating Antibody ◽  
General Method

A general method for induction of the delayed hypersensitive state directed against single protein antigens is described. The method consists of intradermal injection of minute amounts of washed immune precipitates containing the antigen in question. Provided the specific precipitates are formed in the region of antibody excess, maximal sensitivity develops at least 2 to 3 weeks before detectable circulating antibody is formed in guinea pigs against the sensitizing antigen. Neither adjuvant nor killed acid-fast bacteria are required for induction of the delayed hypersensitive state although the degree of sensitization is considerably increased when the sensitizing material is incorporated in Freund's complete adjuvant. Characteristics of the "delayed" as opposed to the "immediate" hypersensitive states in the guinea pig are described and implications of the findings are discussed.

A delayed hypersensitivity reaction to dentine primer in the guinea-pig

1995 ◽  
Vol 23 (5) ◽  
pp. 295-299 ◽  
Author(s):  
K. Katsuno ◽  
A. Manabe ◽  
K. Itoh ◽  
H. Hisamitsu ◽  
S. Wakumoto ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Hypersensitivity Reaction ◽  
Delayed Hypersensitivity ◽  
Delayed Hypersensitivity Reaction

The Development and Interlaboratory Validation of a Quantitative Antibody Capture ELISA for the Measurement of Specific Guinea-pig IgG1: An Alternative to the Passive Cutaneous Anaphylaxis Assay

1996 ◽  
Vol 24 (1) ◽  
pp. 73-79
Author(s):  
Laura S. Babcock ◽  
Thomas T. Kawabata ◽  
Victor S. Moore ◽  
Catherine M. Condo ◽  
Annette Prentø
Keyword(s):  
Guinea Pig ◽  
Passive Cutaneous Anaphylaxis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein Antigens ◽  
Capture Elisa ◽  
Elisa Format ◽  
Interlaboratory Validation ◽  
Antibody Capture ◽  
Development And Validation

Specific guinea-pig IgG1 has traditionally been measured by using an in vivo guinea-pig passive cutaneous anaphylaxis (PCA) assay. This paper describes the development and validation of a quantitative enzyme-linked immunosorbent assay (ELISA) for specific IgG1 against two protein antigens (Alcalase and Enzyme B) as an alternative to the PCA assay. The ELISA format involved a rabbit antibody bound to microtitre plates to capture the antigen. The test sera is added to this, followed sequentially by goat anti-guinea-pig IgG1 and rabbit anti-goat-IgG-alkaline phosphatase conjugate. Aliquots of each serum sample from immunised guinea-pigs were analysed with the ELISA for specific IgG1 titres in three laboratories and were compared to titres determined by using the PCA assay. The findings demonstrate that there is a good correlation between the ELISA and the PCA assay and that the ELISA shows good interlaboratory reproducibility. Thus, the antibody capture ELISA described in this report is a valid and robust replacement for the guinea-pig PCA assay.

Delayed Hypersensitivity in Guinea Pig Gingiva

1975 ◽  
Vol 46 (2) ◽  
pp. 90-101 ◽  
Author(s):  
Efrain Peretzman ◽  
Patrick D. Toto ◽  
Anthony W. Gargiulo
Keyword(s):  
Guinea Pig ◽  
Delayed Hypersensitivity

scholarly journals THE SPECIFICITY OF ALLERGIC REACTIONS

1963 ◽  
Vol 117 (3) ◽  
pp. 401-423 ◽  
Author(s):  
John E. Coe ◽  
S. B. Salvin
Keyword(s):  
Immune Response ◽  
Guinea Pig ◽  
The State ◽  
Delayed Hypersensitivity ◽  
Protein Carrier ◽  
Specific Contact ◽  
Tolerant Animal ◽  
Gastric Feeding

"Gastric feeding" of adult guinea pigs with dinitrochlorobenzene (DCB) resulted in a specific unresponsiveness to sensitization with the specific contact hapten. The more DCB gastric-fed to a guinea pig, the more complete the unresponsiveness to the hapten. When mycobacteria were incorporated into the sensitizing emulsion, the state of unresponsiveness to the dinitrophenyl (DNP) group was less apparent. When animals gastric-fed with DCB were later sensitized with an in vitro conjugate of the hapten combined with a heterologous protein such as dinitrophenyl-hen egg albumin (DNP·HEA), an immune response similar to that in the controls occurred both to the hapten and to the protein carrier. However, when the tolerant animals were sensitized with a conjugate containing a homologous protein carrier such as dinitrophenyl guinea pig serum (DNP·GPS), they showed diminished immune responses in comparison with those in the non-tolerant controls. The presence of circulating anti-DNP antibodies from sensitization with DNP-HEA did not affect the unresponsiveness to the specific contact hapten, regardless of whether these antibodies are present before or after induction of tolerance. Sensitization with picryl chloride (PiCl) (a cross-reacting hapten), either before or after gastric feeding of DCB, did not affect the state of unresponsiveness to DNP. Similarly when the DNP-tolerant animal was sensitized with PiCl, the subsequent immune response was similar to that in the controls; cross-reactions with the DNP group both in the contact and circulating antibody phase occurred at a rate similar to that in the controls. The foregoing relationships can be explained by presuming that, upon the gastric feeding of DCB, an in vivo conjugate is formed with a somatic protein, which determines the basic specificity of the tolerance. Acquired tolerance seems to manifest an immunologic specificity similar to that of delayed hypersensitivity, a relationship not unexpected if delayed hypersensitivity is an early phase of the immune response.

scholarly journals MACROPHAGE-DIGESTED ANTIGEN AS INDUCER OF DELAYED HYPERSENSITIVITY

1971 ◽  
Vol 133 (3) ◽  
pp. 494-505 ◽  
Author(s):  
Margot N. Pearson ◽  
Sidney Raffel
Keyword(s):  
Immune Responses ◽  
Guinea Pig ◽  
Peritoneal Macrophages ◽  
Delayed Hypersensitivity ◽  
Sheep Erythrocytes

Sheep erythrocytes ingested by guinea pig peritoneal macrophages in vitro, and permitted to undergo digestion for various periods, were found after some hours to lose the capacity to induce antibodies while gaining the ability to invoke delayed hypersensitivity. These observations may be related to the known predilection of small molecular immunogens to act as good inducers of delayed reactivity and poor stimulators of antibody. They may be related also to the activity of mycobacterial adjuvant as a vehicle for the induction of delayed hypersensitivity on the basis that this melange activates macrophages to phagocytose and enzymatically degrade macromolecular antigens rapidly. The thesis that small fragments of antigenic molecules may preferentially invoke hypersensitivity can be interpreted on the basis of current concepts of multicellular involvements in immune responses.

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