Haemagglutination-Inhibition Technique with Tanned Erythrocytes Coated with Specific Antibody (Thyroglobulin Antibody)

1968 ◽  
Vol 34 (5) ◽  
pp. 437-454 ◽  
Author(s):  
T. Hjort
Keyword(s):  
Specific Antibody ◽  
Haemagglutination Inhibition ◽  
Thyroglobulin Antibody ◽  
Inhibition Technique

THYROGLOBULIN IN SERUM AFTER TSH STIMULATION IN HYPERTHYROIDISM

1974 ◽  
Vol 75 (4) ◽  
pp. 699-706 ◽  
Author(s):  
T. Hjort ◽  
Th. Friis ◽  
U. Birk Lauridsen ◽  
I. Persson
Keyword(s):  
Thyroid Gland ◽  
Haemagglutination Inhibition ◽  
Serum Thyroxine ◽  
Thyroglobulin Antibody ◽  
Thyroglobulin Level ◽  
Inhibition Technique ◽  
Hyperthyroid Patients ◽  
Tsh Stimulation

ABSTRACT Thyroglobulin in serum was demonstrated by a haemagglutination-inhibition technique and a reversed haemagglutination technique. Circulating thyroglobulin was found in 12 of 15 hyperthyroid patients – both before and during treatment with methylthiouracil – but in none of 12 euthyroid subjects. In three hyperthyroid patients it was not possible to determine thyroglobulin, as thyroglobulin antibody was present in the serum. After TSH stimulation thyroglobulin appeared in the serum of nine of the 12 euthyroid subjects and the thyroglobulin level increased in five of the 12 untreated and six of the 12 treated hyperthyroid patients. The serum thyroxine, T3 uptake in resin and 131I uptake in the thyroid gland at 4 and 24 h were increased after TSH stimulation in all the euthyroid cases; the hyperthyroid patients (both before and during treatment), however, only showed a slight but significant increase in serum thyroxine and 4 h 131I uptake, while the T3 uptake in resin and the 24 h 131I uptake did not rise at all. LATS was found in serum of only five of 15 untreated hyperthyroid patients. No significant changes in the LATS content could be detected during treatment. The increased content of thyroglobulin in the serum of hyperthyroid patients seems to be due neither to greater sensitivity to TSH nor to the influence of LATS.

THE ROLE OF MELATONIN IN THE REGULATION OF THYROTROPHIN SECRETION

1968 ◽  
Vol 57 (3) ◽  
pp. 363-373 ◽  
Author(s):  
J. N. Panda ◽  
C. W. Turner ◽  
Mary Powell
Keyword(s):  
Thyroid Gland ◽  
Marked Decrease ◽  
Haemagglutination Inhibition ◽  
Regulatory Effect ◽  
Hormone Synthesis ◽  
Thyroid Glands ◽  
Tsh Secretion ◽  
The Mean ◽  
Inhibition Technique

ABSTRACT The fact that melatonin, a pineal substance, causes a depression in thyroid function of prepuberal rats has been reported. A possible site of this action on the thyroid gland has been studied by estimating the TSH content of blood and hypophysis in rats from 35 days to 65 days of age. Haemagglutination-inhibition technique has been used to assay very small amounts of TSH in plasma. Rats receiving 100 μg of melatonin daily for 10 days were sacrificed at 35, 45, 55 and 65 days of age. The mean thyroid weight of each group was markedly higher than that of the corresponding controls. The plasma TSH/ml level was higher in experimental groups, with a marked decrease (0.10 > P > 0.05) in TSH/mg of hypophysis (wet), especially at 45 and 55 days of age. Rats similarly treated with 400 μg/100 g body weight of tapazole daily for 10 days and sacrificed at 45 and 55 days of age showed higher plasma and lower hypophyseal TSH levels than the controls and the results were comparable to those of the melatonin treated groups. The dry-fat-free tissue of the thyroid glands of the melatonin treated groups were higher than the corresponding controls and their DNA content was significantly higher (0.050 > P > 0.025) also, indicating hyperplasia and hypertrophy of the thyroid glands resulting from the action of melatonin. The histological picture of the melatonin treated animals showed goitrogenic effect. It may be concluded from these data that melatonin exerts its regulatory effect on TSH secretion directly acting on the thyroid gland and in some way inhibiting thyroid hormone synthesis or release.

scholarly journals SPECIFIC ANTIBODY RESPONSE IN BACKYARD CHICKENS TO NEWCASTLE DISEASE THERMOSTABLE LIVE VACCINE

2017 ◽  
Vol 14 (2) ◽  
pp. 141-144
Author(s):  
S. Baksi ◽  
P. Puwar ◽  
N. Rao ◽  
M. Khan
Keyword(s):  
Antibody Response ◽  
Specific Antibody ◽  
Haemagglutination Inhibition ◽  
Vaccination Schedule ◽  
Economic Losses ◽  
Specific Antibody Response ◽  
Backyard Poultry ◽  
Poultry Disease ◽  
Backyard Chickens ◽  
Thermostable Vaccine

    Newcastledisease (ND) is widely known poultry disease all over the world. It causes high economic losses even in backyard poultry. The purpose of present study was to estimate specific antibody response of backyard poultry chickens to thermostable vaccine of ND. Presence of antibodies before vaccination was determined by haemagglutination-inhibition (HI) test and vaccine was inoculated intraoccularly to birds of vaccinated group. Blood was withdrawn at regular intervals up to 4 months and generation of specific antibodies to the ND antigen was measured by HI test. The results showed significant increase in antibody titre at 28 days time in vaccinated birds and decrease was found after three months of vaccination in the birds. The study suggests that it is necessary to follow vaccination schedule in the backyard chickens, as they are highly susceptible to ND.

A Rapid Turbidimetric Determination of Fibrinogen Degradation Products (FDP)

1978 ◽  
Vol 39 (01) ◽  
pp. 012-021 ◽  
Author(s):  
H W Verbruggen ◽  
J M C Wessels
Keyword(s):  
Human Serum ◽  
Degradation Products ◽  
Haemagglutination Inhibition ◽  
Serum Samples ◽  
Fibrinogen Degradation Products ◽  
Turbidimetric Method ◽  
Antigen Antibody ◽  
Inhibition Technique

SummaryA rapid and simple turbidimetric determination of fibrinogen degradation products is described. This method is based on the increase of the turbidity due to the formation of precipitating antigen-antibody complexes after addition of rabbit antihuman fibrinogen antiserum to human serum. The increase in turbidity correlates very well with results obtained with the haemagglutination inhibition technique and has the advantage of a more objective quantitation. The reproducibility appears to be quite good. The turbidimetric method is independent of the colour of serum samples.

scholarly journals Haemagglutination inhibition technique for the demonstration of sulphones in urine

1981 ◽  
Vol 52 (3) ◽  
Author(s):  
H. HUIKESHOVEN ◽  
MADELEINE DE WIT ◽  
T. A. EGGELTE ◽  
J. E. LANDHEER ◽  
D. L. LEIKER
Keyword(s):  
Haemagglutination Inhibition ◽  
Inhibition Technique

scholarly journals Antibody against viruses in maternal and cord sera: specific antibody is concentrated on the fetal side of the circulation

1982 ◽  
Vol 89 (2) ◽  
pp. 303-310 ◽  
Author(s):  
P. D. Griffiths ◽  
S. I. Berney ◽  
S. Argent ◽  
R. B. Heath
Keyword(s):  
Herpes Simplex ◽  
Influenza A Virus ◽  
Specific Antibody ◽  
Measles Virus ◽  
Influenza A ◽  
Complement Fixation ◽  
Haemagglutination Inhibition ◽  
Influenza B Virus ◽  
Influenza B ◽  
B Virus

SUMMARYPaired maternal and cord sera from 100 pregnancies were tested for antibodies against herpes simplex virus, measles virus and respiratory syncytial virus by complement fixation and for antibodies against rubella virus, influenza A virus and influenza B virus by haemagglutination-inhibition. For four viruses (herpes simplex, measles, respiratory syncytial and rubella) higher levels of antibody were found in cord than in maternal sera. There was no difference between maternal and cord serum titres against influenza B virus but significantly higher levels of antibody against influenza A virus were found in maternal sera than in cord sera. This discrepancy was investigated by measuring antibodies against the surface antigens of influenza A by a complement fixation technique, and by single radial haemolysis. Both methods showed a preponderance of virus-specific antibody in cord sera. We conclude that IgG antibodies against most, if not all, viruses are concentrated on the fetal side of the circulation, but that conventional haemagglutination-inhibition techniques may fail to detect this difference.

OBSERVATIONS ON A HAEMAGGLUTINATION—INHIBITION TECHNIQUE FOR ESTIMATING HUMAN GROWTH HORMONE

1963 ◽  
Vol 26 (2) ◽  
pp. 219-231 ◽  
Author(s):  
P. J. O'CONNOR ◽  
L. G. SKINNER
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Haemagglutination Inhibition ◽  
Human Growth ◽  
Mean Value ◽  
Normal Adult ◽  
Adult Human ◽  
Human Sera ◽  
The Mean ◽  
Inhibition Technique

SUMMARY The haemagglutination—inhibition technique has been examined as a method of estimating human growth hormone (HGH) and the need for rigid standardization of the procedures involved is stressed. Examination of antisera to a Raben type preparation by immunodiffusion and haemagglutination—inhibition procedures showed the presence of antibodies to albumin and γ-globulin as well as to HGH. The presence of these contaminating antibodies did not appear to interfere with the endpoints obtained in the haemagglutination—inhibition reactions. Within its limitations the technique has been found suitable for the assay of solutions of purified HGH. The mean level of HGH in six normal adult human sera was estimated as 261 ± 23·6 μg./l. (± s.e.) which is similar to the values obtained by other workers, but the validity of this mean value is questioned.

scholarly journals ELISA Inhibition Technique for the Demonstration of Sulphones in Body Fluids I. Sulphones Specific Antibody-enzyme Conjugate

1979 ◽  
Vol 50 (4) ◽  
Author(s):  
HAN HUIKESHOVEN ◽  
MADELEINE de WIT ◽  
ANYA SOETERS ◽  
TEUNIS A. EGGELTE ◽  
J. E. LANDHEER ◽  
...  
Keyword(s):  
Specific Antibody ◽  
Body Fluids ◽  
Enzyme Conjugate ◽  
Elisa Inhibition ◽  
Inhibition Technique
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