An Organ Culture Study of Frog Renal Tumor and its Effects on Normal Frog Kidney in vitro

Oncology ◽  
1973 ◽  
Vol 28 (6) ◽  
pp. 536-552 ◽  
Author(s):  
D.M. Morek
2006 ◽  
Vol 22 (4) ◽  
pp. 968-979 ◽  
Author(s):  
Agata Baczynska ◽  
Peter Funch ◽  
Jens Fedder ◽  
Hans Jørgen Knudsen ◽  
Svend Birkelund ◽  
...  

2013 ◽  
Vol 31 (10) ◽  
pp. 1514-1519 ◽  
Author(s):  
Zhu Dai ◽  
Kanghua Li ◽  
Zhiwei Chen ◽  
Ying Liao ◽  
Lezhong Yang ◽  
...  

1971 ◽  
Vol 68 (1_Suppl) ◽  
pp. S27-S40 ◽  
Author(s):  
T. Kobayashi ◽  
T. Kigawa ◽  
M. Mizuno ◽  
T. Watanabe

ABSTRACT There are several in vitro methods to analyse the function of the adenohypophysis or the mechanisms of its regulation. The present paper deals with single cell culture, organ culture and short term incubation techniques by which the morphology and gonadotrophin-secreting function of the adenohypophysis were studied. In trypsin-dispersed cell culture, the adenohypophysial cells showed extensive propagation to form numerous cell colonies and finally develop into a confluent monolayer cell sheet covering completely the surface of culture vessels. Almost all of the cultured cells, however, became chromophobic, at least at the end of the first week of cultivation, when gonadotrophin was detectable neither in the culture medium nor in the cells themselves. After the addition of the hypothalamic extract, gonadotrophin became detectable again, and basophilic or PAS-positive granules also reappeared within the cells, suggesting that the gonadotrophs were stimulated by the extract to produce gonadotrophin. In organ culture and short term incubation, the incorporation of [3H] leucine into the adenohypophysial cells in relation to the addition of hypothalamic extract was examined. It was obvious that the ability to incorporate [3H] leucine into the gonadotrophs in vitro was highly dependent upon the presence of the hypothalamic extract.


PLoS ONE ◽  
2018 ◽  
Vol 13 (2) ◽  
pp. e0192884 ◽  
Author(s):  
Hiroyuki Sanjo ◽  
Mitsuru Komeya ◽  
Takuya Sato ◽  
Takeru Abe ◽  
Kumiko Katagiri ◽  
...  

2007 ◽  
Vol 330-332 ◽  
pp. 877-880 ◽  
Author(s):  
E.S. Thian ◽  
J. Huang ◽  
Serena Best ◽  
Zoe H. Barber ◽  
William Bonfield

Crystalline hydroxyapatite (HA) and 0.8 wt.% silicon-substituted HA (SiHA) thin films were produced using magnetron co-sputtering. These films were subjected to contact angle measurements and in vitro cell culture study using human osteoblast-like (HOB) cells. A wettability study showed that SiHA has a lower contact angle, and thus is more hydrophilic in nature, as compared to HA. Consequently, enhanced cell growth was observed on SiHA at all time-points. Furthermore, distinct and well-developed actin filaments could be seen within HOB cells on SiHA. Thus, this work demonstrated that the surface properties of the coating may be modified by the substitution of Si into the HA structure.


1994 ◽  
Vol 32 (3) ◽  
pp. 184-187 ◽  
Author(s):  
Stephen J. Fortunato ◽  
Ramkumar Menon ◽  
Kenneth F. Swan ◽  
Timothy W. Lyden
Keyword(s):  

1972 ◽  
Vol 50 (7) ◽  
pp. 1627-1631 ◽  
Author(s):  
K. S. Bawa ◽  
R. F. Stettler

Female catkin primordia of black cottonwood (Populus trichocarpa T. & G. ex Hook.) were cultured for 70 days on a modified Murashige and Skoog's (1962) medium in vitro. Explants 2–3 mm long, and with bud scales removed, gave the best results, many of them developing floral structures characteristic of the female sex. There was a general tendency to callus formation with increasing age of the culture, occasionally followed by a reversal to vegetative growth. Catkin primordia raised on Wolter's medium without auxin or kinetin, but with 6-benzylaminopurine, and at 250 ft-c for a 16-h photoperiod, proliferated axillary shoots in loco of pistils.


1992 ◽  
Vol 24 (3) ◽  
pp. 125-128 ◽  
Author(s):  
Chang-Ping Lin ◽  
Matthias Böhnke ◽  
Jorg Draeger

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