scholarly journals Repair of avascular meniscal injuries using juvenile meniscal fragments: An in vitro organ culture study

2013 ◽  
Vol 31 (10) ◽  
pp. 1514-1519 ◽  
Author(s):  
Zhu Dai ◽  
Kanghua Li ◽  
Zhiwei Chen ◽  
Ying Liao ◽  
Lezhong Yang ◽  
...  
Keyword(s):  
Organ Culture ◽  
Culture Study ◽  
In Vitro Organ Culture

scholarly journals Morphology of human Fallopian tubes after infection with Mycoplasma genitalium and Mycoplasma hominis—in vitro organ culture study

2006 ◽  
Vol 22 (4) ◽  
pp. 968-979 ◽  
Author(s):  
Agata Baczynska ◽  
Peter Funch ◽  
Jens Fedder ◽  
Hans Jørgen Knudsen ◽  
Svend Birkelund ◽  
...  
Keyword(s):  
Organ Culture ◽  
Mycoplasma Hominis ◽  
Mycoplasma Genitalium ◽  
Fallopian Tubes ◽  
Culture Study ◽  
In Vitro Organ Culture

The Influence of Fluoride Exposure on Dentin Mineralization Using an in Vitro Organ Culture Model

2003 ◽  
Vol 73 (5) ◽  
pp. 470-475 ◽  
Author(s):  
R. Moseley ◽  
R. J. Waddington ◽  
A. J. Sloan ◽  
A. J. Smith ◽  
R. C. Hall ◽  
...  
Keyword(s):  
Organ Culture ◽  
Fluoride Exposure ◽  
Culture Model ◽  
Dentin Mineralization ◽  
In Vitro Organ Culture

Development of an in vitro organ culture model to study transmission of HIV-1 in the female genital tract

10.1038/74743 ◽  
2000 ◽  
Vol 6 (4) ◽  
pp. 475-479 ◽  
Author(s):  
Kelly B. Collins ◽  
Bruce K. Patterson ◽  
Gregory J. Naus ◽  
Daniel V. Landers ◽  
Phalguni Gupta
Keyword(s):  
Organ Culture ◽  
Genital Tract ◽  
Female Genital Tract ◽  
Culture Model ◽  
In Vitro Organ Culture ◽  
Hiv 1 ◽  
Female Genital

scholarly journals Adherence of enteroaggregative Escherichia coli to the ileal and colonic mucosa: an in vitro study utilizing the scanning electron microscopy

2011 ◽  
Vol 48 (3) ◽  
pp. 199-204 ◽  
Author(s):  
Jacy Alves Braga de Andrade ◽  
Edna Freymüller ◽  
Ulysses Fagundes-Neto
Keyword(s):  
Escherichia Coli ◽  
Organ Culture ◽  
Colonic Mucosa ◽  
In Vitro Study ◽  
In Vitro Assays ◽  
Electron Microscopic ◽  
In Vitro Organ Culture ◽  
Scanning Electron

CONTEXT: Enteroaggregative Escherichia coli strains have been associated with persistent diarrhea in several developing countries. In vivo procedures with animal models, in vitro assays with cellular lines and in vitro organ culture with intestinal fragments have been utilized to study these bacteria and their pathogenicity. OBJECTIVE: The present experimental research assessed the pathogenic interactions of three enteroaggregative Escherichia coli strains, using the in vitro organ culture, in order to show the adherence to different regions of both, the ileal and the colonic mucosa and demonstrate possible mechanisms that could have the participation in the prolongation of diarrheiogenic process. METHODS: This study used intestinal fragments from terminal ileum and colon that were excised from pediatric patients undergoing intestinal surgeries and from adult patients that underwent to colonoscopic procedures. Each strain was tested with three intestinal fragments for each region. Tissue was fixed for scanning electron microscopic analysis. RESULTS: These bacteria colonized ileal and colonic mucosa in the typical stacked-brick configuration in the ileum and colon. In both regions, the strains were seen over a great amount of mucus and sometimes over the intact epithelium. In some regions, there is a probable evidence of effacement of the microvilli. It was possible to see adhered to the intestinal surface, bacteria fimbrial structures that could be responsible for the adherence process. CONCLUSION: In order to cause diarrhea, enteroaggregative Escherichia coli strains adhere to the intestinal mucosa, create a mucoid biofilm on the small bowel surface that could justify the digestive-absorptive abnormalities and consequently, prolonging the diarrhea.

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