New Carrier Protein(s) of Folic Acid in Human Serum

1972 ◽  
Vol 48 (3) ◽  
pp. 145-150 ◽  
Author(s):  
T. Markkanen ◽  
R.-L Pajula ◽  
S. Virtanen ◽  
P. Himanen
Keyword(s):  
Folic Acid ◽  
Human Serum ◽  
Protein S ◽  
Carrier Protein

Transferrin, the Third Carrier Protein of Folic Acid Activity in Human Serum

1972 ◽  
Vol 48 (4) ◽  
pp. 213-217 ◽  
Author(s):  
T. Markkanen ◽  
S. Virtanen ◽  
P. Himanen ◽  
R.-L Pajula
Keyword(s):  
Folic Acid ◽  
Human Serum ◽  
Carrier Protein ◽  
The Third

[Acyl-carrier-protein] S-malonyltransferase

1996 ◽  
pp. 795-799
Author(s):  
Dietmar Schomburg ◽  
Dörte Stephan
Keyword(s):  
Acyl Carrier Protein ◽  
Protein S ◽  
Carrier Protein

scholarly journals Evidence Against Transferrin as a Binder of Either Vitamin B12 or Folic Acid

Blood ◽  
1974 ◽  
Vol 43 (5) ◽  
pp. 767-768 ◽  
Author(s):  
Elizabeth Jacob ◽  
Victor Herbert
Keyword(s):  
Folic Acid ◽  
Vitamin B12 ◽  
Carrier Protein ◽  
Human Transferrin

Abstract Human transferrin is not a carrier protein for either folic acid or vitamin B12.

scholarly journals Development of a Sensitive Chemiluminescence Immunoassay for the Quantification of Folic Acid in Human Serum

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Xiang Chen ◽  
Qiyang Zhou ◽  
Ting Zhang ◽  
ChunXin Wang ◽  
Zheng Yu ◽  
...  
Keyword(s):  
Folic Acid ◽  
Horseradish Peroxidase ◽  
Human Serum ◽  
Magnetic Beads ◽  
Megaloblastic Anemia ◽  
Human Growth ◽  
Cross Reactivity ◽  
Alkaline Condition ◽  
Folate Binding Protein ◽  
Chemiluminescence Immunoassay

Folic acid (FA) is an important vitamin for human growth, especially for pregnant women. FA deficiency is associated with megaloblastic anemia, neural tube defects, cardiovascular diseases, irritability, diarrhea, and psychiatric disorders. Normally, FA molecules bind to folate-binding protein (FBP) in the serum as complex. Before quantify the FA concentration, a releasing procedure should be conducted. Alkaline condition and tris(2-carboxyethyl)phosphine (TCEP) are used to release binding FA to freeing state. In this work, a chemiluminescence immunoassay (CLIA) for human serum FA was established by competition model. Streptavidin (SA) was labeled to magnetic beads by an 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDAC/NHS) method. Activated biotin molecules were labeled to FBP molecules purified from milk. FA was labeled to horseradish peroxidase (HRP) by EDAC to activate the FA molecules. The pretreated samples or standards were added into the reaction tube with biotin-FBP and FA-horseradish peroxidase (HRP), FA in the sample compete with FA-HRP for binding to biotin-FBP, the signal is inversely proportional to the FA concentration. The method established shows good thermostability and performance. The limitation of detection (LOD) is 0.44 ng/mL. The intra-assay coefficient of variation (CV) is 3.6%–7.1%, the interassay CV is 4.2%–7.5%, and the recovery rate is 92.1%–103.5%. Cross reactivity (CR) was remarkably low with aminopterin, folinic acid, and methotrexate. The method shows good correlation with the FA CLIA product from Beckman Coulter; the equation is y = 0.9618x−0.1434 while the R2 value is 0.9224. The established method is sensitive, rapid, and accurate which can fully satisfy for the clinical requirement.

Effect of Carrier Surface Modification on the Resultant Antibody to Folic Acid

2012 ◽  
Vol 581-582 ◽  
pp. 77-80
Author(s):  
Qiu Shi Tang ◽  
Hong Tao Lei ◽  
Long Bin Huang ◽  
Yuan Ming Sun ◽  
Jin Yi Yang
Keyword(s):  
Surface Modification ◽  
Folic Acid ◽  
Ultraviolet Spectrum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Carrier Protein ◽  
Coupling Ratio ◽  
Hexamethylene Diamine ◽  
Artificial Antigen ◽  
Sds Page ◽  
Surface Modified

For the generation of antibodies against small hapten molecules, the hapten is cross-linked with some carrier protein to make it immunogenic. However, it was rarely systematically studied about the effect of modified carrier protein on the obtained antibody nature. In this study, folic acid as the model hapten was coupled to natural and surface-modified bovine serum albumin (BSA) using modifying agent amidocaproic acid (ACA) and hexamethylene diamine (HDA). The three immunogens of FA-BSA, FA-ACA-BSA, FA-HDA-BSA were confirmed by SDS-PAGE and ultraviolet spectrum (UV). The Balb/C mice were immunized with the artificial antigen to obtain three specific antibody with indirect competitive enzyme-linked immunosorbent assay (icELISA) method to compare the performance of antibodies obtained based on different imunogen. The results indicate that the conjugates were successfully synthesized and the coupling ratio from the high to the low was HDA-BSA > ACA-BSA > BSA, while the titer of antibody was ACA-BSA > BSA >HDA-BSA and the IC50was HDA-BSA < ACA-BSA < BSA. This suggests that sensitivity of antibody was improved by cationizing the carrier protein.

scholarly journals Folic acid receptor-targeted human serum albumin nanoparticle formulation of cabazitaxel for tumor therapy

2018 ◽  
Vol Volume 14 ◽  
pp. 135-148 ◽  
Author(s):  
Yating Sun ◽  
Yarong Zhao ◽  
Shanshan Teng ◽  
Fei Hao ◽  
Huan Zhang ◽  
...  
Keyword(s):  
Folic Acid ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Tumor Therapy ◽  
Acid Receptor ◽  
Nanoparticle Formulation ◽  
Folic Acid Receptor

Carrier Proteins of Folic Acid Activity in Human Serum

1971 ◽  
Vol 45 (2) ◽  
pp. 106-111 ◽  
Author(s):  
T. Markkanen ◽  
O. Peltola
Keyword(s):  
Folic Acid ◽  
Human Serum ◽  
Carrier Proteins

scholarly journals Adsorptive stripping voltammetric assay of folic acid in human serum

1988 ◽  
Vol 6 (6-8) ◽  
pp. 743-747 ◽  
Author(s):  
J.M.Fernandez Alvarez ◽  
A.Costa Garcia ◽  
A.J.Miranda Ordieres ◽  
Paulino Tuñón Blanco
Keyword(s):  
Folic Acid ◽  
Human Serum ◽  
Voltammetric Assay
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