Early Sacral Neural Crest Migration in Dominant megacolon Mouse Embryos

2008 ◽  
Vol 5 (3) ◽  
pp. 69-79 ◽  
Author(s):  
W.Y. Tam ◽  
X. Wang ◽  
L. Wang ◽  
L.H. Bao ◽  
W.Y. Chan
Keyword(s):  
Neural Crest ◽  
Mouse Embryos ◽  
Dominant Megacolon ◽  
Sacral Neural Crest ◽  
Neural Crest Migration

Early Migration of Sacral Neural Crest Cells in Mouse Embryos

2006 ◽  
Vol 4 (4) ◽  
pp. 189-201 ◽  
Author(s):  
M. Dong ◽  
X. Wang ◽  
A.K. Chan ◽  
A.J. Burns ◽  
W.Y. Chan
Keyword(s):  
Neural Crest ◽  
Neural Crest Cells ◽  
Mouse Embryos ◽  
Early Migration ◽  
Sacral Neural Crest

scholarly journals Vital dye labelling demonstrates a sacral neural crest contribution to the enteric nervous system of chick and mouse embryos

Development ◽  
1991 ◽  
Vol 111 (4) ◽  
pp. 857-866 ◽  
Author(s):  
G.N. Serbedzija ◽  
S. Burgan ◽  
S.E. Fraser ◽  
M. Bronner-Fraser
Keyword(s):  
Nervous System ◽  
Neural Crest ◽  
Enteric Nervous System ◽  
Neural Tube ◽  
Neural Crest Cells ◽  
Mouse Embryos ◽  
Chick Embryos ◽  
Vital Dye ◽  
Dorsal Portion ◽  
Sacral Neural Crest

We have used the vital dye, DiI, to analyze the contribution of sacral neural crest cells to the enteric nervous system in chick and mouse embryos. In order to label premigratory sacral neural crest cells selectively, DiI was injected into the lumen of the neural tube at the level of the hindlimb. In chick embryos, DiI injections made prior to stage 19 resulted in labelled cells in the gut, which had emerged from the neural tube adjacent to somites 29–37. In mouse embryos, neural crest cells emigrated from the sacral neural tube between E9 and E9.5. In both chick and mouse embryos, DiI-labelled cells were observed in the rostral half of the somitic sclerotome, around the dorsal aorta, in the mesentery surrounding the gut, as well as within the epithelium of the gut. Mouse embryos, however, contained consistently fewer labelled cells than chick embryos. DiI-labelled cells first were observed in the rostral and dorsal portion of the gut. Paralleling the maturation of the embryo, there was a rostral-to-caudal sequence in which neural crest cells populated the gut at the sacral level. In addition, neural crest cells appeared within the gut in a dorsal-to-ventral sequence, suggesting that the cells entered the gut dorsally and moved progressively ventrally. The present results resolve a long-standing discrepancy in the literature by demonstrating that sacral neural crest cells in both the chick and mouse contribute to the enteric nervous system in the postumbilical gut.

An experimental study on neural crest migration in Barbus conchonius (Cyprinidae, Teleostei), with special reference to the origin of the enteroendocrine cells

Development ◽  
1981 ◽  
Vol 62 (1) ◽  
pp. 309-323
Author(s):  
C. H. J. Lamers ◽  
J. W. H. M. Rombout ◽  
L. P. M. Timmermans
Keyword(s):  
Neural Crest ◽  
Neural Tube ◽  
Ganglion Cells ◽  
Neural Crest Cells ◽  
Enteroendocrine Cells ◽  
Pigment Cells ◽  
Transplantation Technique ◽  
Spinal Ganglion Cells ◽  
Neural Crest Origin ◽  
Neural Crest Migration

A neural crest transplantation technique is described for fish. As in other classes ofvertebrates, two pathways of neural crest migration can be distinguished: a lateroventral pathway between somites and ectoderm, and a medioventral pathway between somites and neural tube/notochord. In this paper evidence is presented for a neural crest origin of spinal ganglion cells and pigment cells, and indication for such an origin is obtained for sympathetic and enteric ganglion cells and for cells that are probably homologues to adrenomedullary and paraganglion cells in the future kidney area. The destiny of neural crest cells near the developing lateral-line sense organs is discussed. When grafted into the yolk, neural crest cells or neural tube cells appear to differentiate into ‘periblast cells’; this suggests a highly activating influence of the yolk. Many neural crest cells are found around the urinary ducts and, when grafted below the notochord, even within the urinary duct epithelium. These neural crest cells do not invade the gut epithelium, even when grafted adjacent to the developing gut. Consequently enteroendocrine cells in fish are not likely to have a trunkor rhombencephalic neural crest origin. Another possible origin of these cells will be proposed.

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