Mammalian neural crest cells participate in normal embryonic development on microinjection into post-implantation mouse embryos

Nature ◽  
1985 ◽  
Vol 318 (6042) ◽  
pp. 181-183 ◽  
Author(s):  
Rudolf Jaenisch
Keyword(s):  
Embryonic Development ◽  
Neural Crest ◽  
Neural Crest Cells ◽  
Mouse Embryos ◽  
Post Implantation ◽  
Normal Embryonic Development

Early Migration of Sacral Neural Crest Cells in Mouse Embryos

2006 ◽  
Vol 4 (4) ◽  
pp. 189-201 ◽  
Author(s):  
M. Dong ◽  
X. Wang ◽  
A.K. Chan ◽  
A.J. Burns ◽  
W.Y. Chan
Keyword(s):  
Neural Crest ◽  
Neural Crest Cells ◽  
Mouse Embryos ◽  
Early Migration ◽  
Sacral Neural Crest

scholarly journals Vital dye labelling demonstrates a sacral neural crest contribution to the enteric nervous system of chick and mouse embryos

Development ◽  
1991 ◽  
Vol 111 (4) ◽  
pp. 857-866 ◽  
Author(s):  
G.N. Serbedzija ◽  
S. Burgan ◽  
S.E. Fraser ◽  
M. Bronner-Fraser
Keyword(s):  
Nervous System ◽  
Neural Crest ◽  
Enteric Nervous System ◽  
Neural Tube ◽  
Neural Crest Cells ◽  
Mouse Embryos ◽  
Chick Embryos ◽  
Vital Dye ◽  
Dorsal Portion ◽  
Sacral Neural Crest

We have used the vital dye, DiI, to analyze the contribution of sacral neural crest cells to the enteric nervous system in chick and mouse embryos. In order to label premigratory sacral neural crest cells selectively, DiI was injected into the lumen of the neural tube at the level of the hindlimb. In chick embryos, DiI injections made prior to stage 19 resulted in labelled cells in the gut, which had emerged from the neural tube adjacent to somites 29–37. In mouse embryos, neural crest cells emigrated from the sacral neural tube between E9 and E9.5. In both chick and mouse embryos, DiI-labelled cells were observed in the rostral half of the somitic sclerotome, around the dorsal aorta, in the mesentery surrounding the gut, as well as within the epithelium of the gut. Mouse embryos, however, contained consistently fewer labelled cells than chick embryos. DiI-labelled cells first were observed in the rostral and dorsal portion of the gut. Paralleling the maturation of the embryo, there was a rostral-to-caudal sequence in which neural crest cells populated the gut at the sacral level. In addition, neural crest cells appeared within the gut in a dorsal-to-ventral sequence, suggesting that the cells entered the gut dorsally and moved progressively ventrally. The present results resolve a long-standing discrepancy in the literature by demonstrating that sacral neural crest cells in both the chick and mouse contribute to the enteric nervous system in the postumbilical gut.

scholarly journals Early Development of The Trunk Neural Crest Cells in the Egyptian Cobra Naja H. Haje

2021 ◽  
Author(s):  
Eraqi R. Khannoon ◽  
Christian Alvarado ◽  
Maria Elena Elena de Bellard
Keyword(s):  
Embryonic Development ◽  
Neural Crest ◽  
Early Development ◽  
Cancer Metastasis ◽  
Epithelial To Mesenchymal Transition ◽  
Early Stage ◽  
Neural Crest Cells ◽  
Migratory Behavior ◽  
Mesenchymal Transition ◽  
Trunk Neural Crest

Abstract Background: Trunk neural crest cells (TNCC) are representing a model for epithelial to mesenchymal transition, this correlates the importance of studying the migration of these cells to cancer metastasis. Reptiles are unique group of animals being very morphologically diverse and their close position to synapsid leading to mammals. Recently, more publications focused on the migratory behavior of trunk NCC during embryonic development of squamates. Only one colubrid snake has been studied so far regarding the NCC migration. Results: Here we follow the migratory behavior of TNCC with HNK1 in the elapid snake Naja h. haje from early stage to 14 days postoviposition. Comparing the colubrid snake with the Egyptian cobra showed that both snakes overall follow the same TNCC migratory pathways of both birds and mammals by following the rostral and avoiding the caudal portions of the somites. Conclusions: First, TNCC intra-somitic migration as observed in turtles supports a contributing role for TNCC to scale precursors. Second, our observation of significant numbers of migrating TNCC in the intersomitic pathway suggest interesting evolutionary differences. Together, our present results of the Egyptian cobra in combination with those on a colubrid and turtle supports intersomitic TNCC as a unique reptile phenomena.

Dissociation and Reaggregation of Embryonic cells of Triturus alpestris

Development ◽  
1955 ◽  
Vol 3 (3) ◽  
pp. 251-255
Author(s):  
M. Feldman
Keyword(s):  
Embryonic Development ◽  
Neural Crest ◽  
Cell Movement ◽  
Neural Crest Cells ◽  
Embryonic Cells ◽  
Experimental Conditions ◽  
Developmental Processes ◽  
Triturus Alpestris ◽  
And Migration

Cell movement and migration seem to play an important role in the formation of tissue-patterns during embryogenesis. Phenomena such as the appearance of loosely attached cells in the mesoderm, or of the freely migrating neural crest cells, are quite common in embryonic development. Since the tissues of adult organs are mostly rather compact in structure it seems that the capacity of isolated or loosely arranged cells to reassociate is an obligatory condition for many developmental processes. This capacity, under experimental conditions, was extensively studied by Holtfreter (1947, 1948). He has shown that cells of newt blastulae and early gastrulae can be made to dissociate and can then become reaggregated and proceed with their morphogenetic development. His experiments were carried out, for the most part, either with cells prior to histogenetic determination or with determined cells of a single tissue.

Expression of Schwann cell markers by mammalian neural crest cells in vitro

Development ◽  
1989 ◽  
Vol 105 (2) ◽  
pp. 251-262 ◽  
Author(s):  
L.C. Smith-Thomas ◽  
J.W. Fawcett
Keyword(s):  
Monoclonal Antibody ◽  
Schwann Cell ◽  
Embryonic Development ◽  
Neural Crest ◽  
Schwann Cells ◽  
Neural Crest Cell ◽  
Neural Crest Cells ◽  
Similar Proportion ◽  
Cell Phenotype ◽  
Cell Markers

During embryonic development, neural crest cells differentiate into a wide variety of cell types including Schwann cells of the peripheral nervous system. In order to establish when neural crest cells first start to express a Schwann cell phenotype immunocytochemical techniques were used to examine rat premigratory neural crest cell cultures for the presence of Schwann cell markers. Cultures were fixed for immunocytochemistry after culture periods ranging from 1 to 24 days. Neural crest cells were identified by their morphology and any neural tube cells remaining in the cultures were identified by their epithelial morphology and immunocytochemically. As early as 1 to 2 days in culture, approximately one third of the neural crest cells stained with m217c, a monoclonal antibody that appears to recognize the same antigen as rat neural antigen-1 (RAN-1). A similar proportion of cells were immunoreactive in cultures stained with 192-IgG, a monoclonal antibody that recognizes the rat nerve growth factor receptor. The number of immunoreactive cells increased with time in culture. After 16 days in culture, nests of cells, many of which had a bipolar morphology, were present in the area previously occupied by neural crest cells. The cells in the nests were often associated with neurons and were immunoreactive for m217c, 192-IgG and antibody to S-100 protein and laminin, indicating that the cells were Schwann cells. At all culture periods examined, neural crest cells did not express glial fibrillary acidic protein. These results demonstrate that cultured premigratory neural crest cells express early Schwann cell markers and that some of these cells differentiate into Schwann cells. These observations suggest that some neural crest cells in vivo may be committed to forming Schwann cells and will do so provided that they then proceed to encounter the correct environmental cues during embryonic development.

scholarly journals Misexpression of BRE gene in the developing chick neural tube affects neurulation and somitogenesis

2015 ◽  
Vol 26 (5) ◽  
pp. 978-992 ◽  
Author(s):  
Guang Wang ◽  
Yan Li ◽  
Xiao-Yu Wang ◽  
Manli Chuai ◽  
John Yeuk-Hon Chan ◽  
...  
Keyword(s):  
Chick Embryo ◽  
Embryonic Development ◽  
Neural Crest ◽  
Embryo Development ◽  
Neural Tube ◽  
Neural Crest Cells ◽  
Chick Embryos ◽  
Early Chick Embryo

This is the first study of the role of BRE in embryonic development using early chick embryos. BRE is expressed in the developing neural tube, neural crest cells, and somites. BRE thus plays an important role in regulating neurogenesis and indirectly somitogenesis during early chick embryo development.

scholarly journals Cross Talk between Adhesion Molecules: Control of N-cadherin Activity by Intracellular Signals Elicited by β1 and β3 Integrins in Migrating Neural Crest Cells

1997 ◽  
Vol 137 (7) ◽  
pp. 1663-1681 ◽  
Author(s):  
Frédérique Monier-Gavelle ◽  
Jean-Loup Duband
Keyword(s):  
Cell Migration ◽  
Embryonic Development ◽  
Neural Crest ◽  
Neural Crest Cell ◽  
Neural Crest Cells ◽  
Dependent Manner ◽  
Adhesion Receptors ◽  
Surface Distribution ◽  
Intracellular Signals ◽  
Intercellular Contacts

During embryonic development, cell migration and cell differentiation are associated with dynamic modulations both in time and space of the repertoire and function of adhesion receptors, but the nature of the mechanisms responsible for their coordinated occurrence remains to be elucidated. Thus, migrating neural crest cells adhere to fibronectin in an integrin-dependent manner while maintaining reduced N-cadherin–mediated intercellular contacts. In the present study we provide evidence that, in these cells, the control of N-cadherin may rely directly on the activity of integrins involved in the process of cell motion. Prevention of neural crest cell migration using RGD peptides or antibodies to fibronectin and to β1 and β3 integrins caused rapid N-cadherin–mediated cell clustering. Restoration of stable intercellular contacts resulted essentially from the recruitment of an intracellular pool of N-cadherin molecules that accumulated into adherens junctions in tight association with the cytoskeleton and not from the redistribution of a preexisting pool of surface N-cadherin molecules. In addition, agents that cause elevation of intracellular Ca2+ after entry across the plasma membrane were potent inhibitors of cell aggregation and reduced the N-cadherin– mediated junctions in the cells. Finally, elevated serine/ threonine phosphorylation of catenins associated with N-cadherin accompanied the restoration of intercellular contacts. These results indicate that, in migrating neural crest cells, β1 and β3 integrins are at the origin of a cascade of signaling events that involve transmembrane Ca2+ fluxes, followed by activation of phosphatases and kinases, and that ultimately control the surface distribution and activity of N-cadherin. Such a direct coupling between adhesion receptors by means of intracellular signals may be significant for the coordinated interplay between cell–cell and cell–substratum adhesion that occurs during embryonic development, in wound healing, and during tumor invasion and metastasis.

scholarly journals How to distinguish between different cell lineages sharing common markers using combinations of double in-situ-hybridization and immunostaining in avian embryos: CXCR4-positive mesodermal and neural crest-derived cells

2020 ◽  
Vol 155 (1) ◽  
pp. 145-155 ◽  
Author(s):  
Imadeldin Yahya ◽  
Marion Böing ◽  
Beate Brand-Saberi ◽  
Gabriela Morosan-Puopolo
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Embryonic Development ◽  
Neural Crest ◽  
Expression Patterns ◽  
Simultaneous Detection ◽  
Neural Crest Cells ◽  
Cell Lineages ◽  
Wide Range

AbstractCell migration plays a crucial role in early embryonic development. The chemokine receptor CXCR4 has been reported to guide migration of neural crest cells (NCCs) to form the dorsal root ganglia (DRG) and sympathetic ganglia (SG). CXCR4 also plays an important part during the formation of limb and cloacal muscles. NCCs migration and muscle formation during embryonic development are usually considered separately, although both cell lineages migrate in close neighbourhood and have markers in common. In this study, we present a new method for the simultaneous detection of CXCR4, mesodermal markers and NCCs markers during chicken embryo developmental stages HH18–HH25 by combining double whole-mount in situ hybridization (ISH) and immunostaining on floating vibratome sections. The simultaneous detection of CXCR4 and markers for the mesodermal and neural crest cells in multiple labelling allowed us to compare complex gene expression patterns and it could be easily used for a wide range of gene expression pattern analyses of other chicken embryonic tissues. All steps of the procedure, including the preparation of probes and embryos, prehybridization, hybridization, visualization of the double labelled transcripts and immunostaining, are described in detail.

Influence of injected pluripotential (EK) cells on haploid and diploid parthenogenetic development

Development ◽  
1984 ◽  
Vol 80 (1) ◽  
pp. 75-86
Author(s):  
M. H. Kaufman ◽  
M. J. Evans ◽  
E. J. Robertson ◽  
A. Bradley
Keyword(s):  
Embryonic Development ◽  
Somite Stage ◽  
Parthenogenetic Development ◽  
Stage Embryo ◽  
Haploid Embryos ◽  
Post Implantation ◽  
Parthenogenetic Embryos ◽  
Normal Embryonic Development

A number of pluripotential embryo-derived EK cells were introduced into the blastocoele of haploid and diploid parthenogenetic embryos which were subsequently transferred to suitable recipients. At autopsy on day 10 or 11 of pseudopregnancy 22 % of decidua in the diploid series contained somite-stage embryos while an additional 12 % contained abnormal egg cylinder-like sacs. In the haploid series, 7 % of the decidua contained somite-stage embryos and an additional 5 % contained abnormal ‘sacs’. In ‘injected’ diploid and haploid ‘controls’ in which the zonae were pierced with an empty injection pipette 3 % and 0 % respectively of decidua in these two series contained somite-stage embryos, while an additional 17 % and 3 % respectively of decidua in these two series contained abnormal sacs. GPI analysis revealed that the EK cells were incorporated into somite-stage conceptuses in only one third of the diploids and in none of the haploid embryos. Although the presence of EK cells considerably increases the chance of normal embryonic development taking place, a detectable contribution from the EK cells into the resulting somite-stage embryo is apparently not necessary. Possible mechanisms allowing successful early post-implantation development to occur in this study are discussed.

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