Early Migration of Sacral Neural Crest Cells in Mouse Embryos

2006 ◽  
Vol 4 (4) ◽  
pp. 189-201 ◽  
Author(s):  
M. Dong ◽  
X. Wang ◽  
A.K. Chan ◽  
A.J. Burns ◽  
W.Y. Chan
Keyword(s):  
Neural Crest ◽  
Neural Crest Cells ◽  
Mouse Embryos ◽  
Early Migration ◽  
Sacral Neural Crest

scholarly journals Vital dye labelling demonstrates a sacral neural crest contribution to the enteric nervous system of chick and mouse embryos

Development ◽  
1991 ◽  
Vol 111 (4) ◽  
pp. 857-866 ◽  
Author(s):  
G.N. Serbedzija ◽  
S. Burgan ◽  
S.E. Fraser ◽  
M. Bronner-Fraser
Keyword(s):  
Nervous System ◽  
Neural Crest ◽  
Enteric Nervous System ◽  
Neural Tube ◽  
Neural Crest Cells ◽  
Mouse Embryos ◽  
Chick Embryos ◽  
Vital Dye ◽  
Dorsal Portion ◽  
Sacral Neural Crest

We have used the vital dye, DiI, to analyze the contribution of sacral neural crest cells to the enteric nervous system in chick and mouse embryos. In order to label premigratory sacral neural crest cells selectively, DiI was injected into the lumen of the neural tube at the level of the hindlimb. In chick embryos, DiI injections made prior to stage 19 resulted in labelled cells in the gut, which had emerged from the neural tube adjacent to somites 29–37. In mouse embryos, neural crest cells emigrated from the sacral neural tube between E9 and E9.5. In both chick and mouse embryos, DiI-labelled cells were observed in the rostral half of the somitic sclerotome, around the dorsal aorta, in the mesentery surrounding the gut, as well as within the epithelium of the gut. Mouse embryos, however, contained consistently fewer labelled cells than chick embryos. DiI-labelled cells first were observed in the rostral and dorsal portion of the gut. Paralleling the maturation of the embryo, there was a rostral-to-caudal sequence in which neural crest cells populated the gut at the sacral level. In addition, neural crest cells appeared within the gut in a dorsal-to-ventral sequence, suggesting that the cells entered the gut dorsally and moved progressively ventrally. The present results resolve a long-standing discrepancy in the literature by demonstrating that sacral neural crest cells in both the chick and mouse contribute to the enteric nervous system in the postumbilical gut.

scholarly journals PROX1 is a specific and dynamic marker of sacral neural crest cells in the chicken intestine

2019 ◽  
Vol 528 (5) ◽  
pp. 879-889
Author(s):  
Andreia S. Margarido ◽  
Ludovic Le Guen ◽  
Amandine Falco ◽  
Sandrine Faure ◽  
Norbert Chauvet ◽  
...  
Keyword(s):  
Neural Crest ◽  
Neural Crest Cells ◽  
Chicken Intestine ◽  
Sacral Neural Crest
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