Deoxyribonucleic Acid Repair Replication in Pseudomonas aeruginosa after Sublethal Doses of Silver Sulfadiazine

Pathobiology ◽  
1973 ◽  
Vol 39 (6) ◽  
pp. 434-445
Author(s):  
M.S. Wysor ◽  
R.E. Zollinhofer
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Deoxyribonucleic Acid ◽  
Silver Sulfadiazine ◽  
Sublethal Doses

Experimental immunization with attenuated mutants of Pseudomonas aeruginosa

1973 ◽  
Vol 19 (5) ◽  
pp. 573-580
Author(s):  
Delfa Syeklocha
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibody Response ◽  
Specific Antibody ◽  
Complex Medium ◽  
Specific Antibody Response ◽  
Intraperitoneal Route ◽  
Viable Cells ◽  
Sublethal Doses ◽  
Experimental Immunization

Two attenuated mutants of Pseudomonas aeruginosa (PA7), designated P-8 and P-11, were tested for their immunizing potential in mice. One or three sublethal doses of viable cells of either mutant given by the intraperitoneal route did not increase the resistance of the animals even with time, to challenge with the virulent parent form. However, increased survival was obtained in groups of mice immunized with six doses of either a viable or formalinized preparation of the P-8 mutant, injected intraperitoneally or intravenously. An elevated specific antibody response was found only in the sera of animals immunized with the largest doses of P-8 and this antiserum protected normal animals when given intraperitoneally 4 h before challenge.The two mutants appear to be similar, if not identical, with the parent form, with the exception of their slower growth in complex medium and their reduced virulence for mice.

Satellite deoxyribonucleic acid in Pseudomonas aeruginosa

1972 ◽  
Vol 18 (7) ◽  
pp. 1003-1006
Author(s):  
Ruth B. Finley ◽  
J. D. Punch
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Satellite Dna ◽  
Deoxyribonucleic Acid ◽  
The Other ◽  
Clinical Specimens

Deoxyribonucleic acid preparations from two strains of Pseudomonas aeruginosa newly isolated from clinical specimens each contained double-stranded satellite DNA. The satellite DNA in one strain consisted of two components with buoyant densities of 1.705 and 1.712 g cm−3 and comprised 28% of the total extracted DNA. The other strain contained 15% satellite DNA which was composed of components with buoyant densities of 1.711 and 1.718 g cm−3.

scholarly journals Antibacterial ativity of biosynthesized silver nanoparticles against Pseudomonas aeruginosa in vitro

2017 ◽  
Vol 41 (1) ◽  
pp. 60-65
Author(s):  
Ahmad N. A. Salih ◽  
Mohammad J. Eesa
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Silver Sulfadiazine ◽  
Diffusion Method ◽  
Olive Leaves ◽  
Scanning Electron

     This study was conducted for the synthesis of silver nanoparticles by using olive leaves aqueous extract and evaluate its antibacterial activity against Pseudomonas aeruginosa in vitro. The synthesis and characterization of silver nanoparticles was confirmed by Ultra Violet Visible – spectrophotometer and Scanning Electron Microscopy. Well diffusion method was used to show the antibacterial action of silver nanoparticles against Pseudomonas aeruginosa in vitro in comparison with standard antibacterial silver sulfadiazine by using different concentrations of each agent ranged from 12.5-200 μg/ml. The results of this study showed it possible to produce silver nanoparticles in eco-friendly and easy process and UV-Visible absorption spectra of the silver nanoparticles revealed maximum absorbance at 420 and 430 nm. The Scanning Electron Microscopy analysis demonstrated the mean of the silver particles diameter was 26 nm. The antibacterial findings of the synthesized silver nanoparticles against Pseudomonas aeruginosa in vitro showed that the silver nanoparticles were more effective than silver sulfadiazine against Pseudomonas aeruginosa. It could be concluded that olive leaves extract can be used effectively in the production of silver nanoparticles and these synthesized nanoparticles had considerable antibacterial activity against Pseudomonas aeruginosa in vitro.                                                        

Evaluation of the Antimicrobial Efficacy of Urinary Catheters Impregnated With Antiseptics in an In Vitro Urinary Tract Model

2003 ◽  
Vol 24 (7) ◽  
pp. 506-513 ◽  
Author(s):  
Trupti A. Gaonkar ◽  
Lester A. Sampath ◽  
Shanta M. Modak
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Urinary Tract ◽  
Staphylococcus Epidermidis ◽  
Bacterial Colonization ◽  
Silver Sulfadiazine ◽  
Antimicrobial Spectrum

AbstractObjectives:To evaluate the long-term efficacy of urinary Foley catheters (latex and silicone) impregnated with (1) chlorhexidine and silver sulfadiazine (CXS) and (2) chlorhexidine, silver sulfadiazine, and triclosan (CXST) in inhibiting extra-luminal bacterial adherence and to compare their efficacy with that of silver hydrogel latex (SH) and nitrofurazone-treated silicone (NF) catheters.Design:The antimicrobial spectrum of these catheters was evaluated using a zone of inhibition assay. A novel in vitro urinary tract model was developed to study the potential in vivo efficacy of antimicrobial catheters in preventing extraluminal bacterial colonization. The “meatus” was inoculated daily with Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa, and Candida albicans. The “bladder” portion of the model was cultured daily to determine bacterial growth.Results:Both CXS and CXST catheters had a broader antimicrobial spectrum than SH and NF catheters. In the in vitro model, CXST latex and silicone catheters exhibited significantly better efficacy (3 to 25 days) against uropathogens, compared with CXS (1 to 14 days) and control (0 to 5 days) catheters (P = .01). CXST latex catheters exhibited significantly longer protection against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and Pseudomonas aeruginosa, compared with SH catheters (P = .01). CXST silicone catheters resisted colonization with Staphylococcus aureus and Staphylococcus epidermidis for a significantly longer period (23 to 24 days) than did NF catheters (9 to 11 days) (P = .01).Conclusion:Catheters impregnated with synergistic combinations of chlorhexidine, silver sulfadiazine, and triclosan exhibited broad-spectrum, long-term resistance against microbial colonization on their outer surfaces (Infect Control Hosp Epidemiol 2003;24:506-513)

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