Experimental immunization with attenuated mutants of Pseudomonas aeruginosa

1973 ◽  
Vol 19 (5) ◽  
pp. 573-580
Author(s):  
Delfa Syeklocha
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibody Response ◽  
Specific Antibody ◽  
Complex Medium ◽  
Specific Antibody Response ◽  
Intraperitoneal Route ◽  
Viable Cells ◽  
Sublethal Doses ◽  
Experimental Immunization

Two attenuated mutants of Pseudomonas aeruginosa (PA7), designated P-8 and P-11, were tested for their immunizing potential in mice. One or three sublethal doses of viable cells of either mutant given by the intraperitoneal route did not increase the resistance of the animals even with time, to challenge with the virulent parent form. However, increased survival was obtained in groups of mice immunized with six doses of either a viable or formalinized preparation of the P-8 mutant, injected intraperitoneally or intravenously. An elevated specific antibody response was found only in the sera of animals immunized with the largest doses of P-8 and this antiserum protected normal animals when given intraperitoneally 4 h before challenge.The two mutants appear to be similar, if not identical, with the parent form, with the exception of their slower growth in complex medium and their reduced virulence for mice.

scholarly journals Whole Transcriptome Profiling Identifies CD93 and Other Plasma Cell Survival Factor Genes Associated with Measles-Specific Antibody Response after Vaccination

PLoS ONE ◽  
2016 ◽  
Vol 11 (8) ◽  
pp. e0160970 ◽  
Author(s):  
Iana H. Haralambieva ◽  
Michael T. Zimmermann ◽  
Inna G. Ovsyannikova ◽  
Diane E. Grill ◽  
Ann L. Oberg ◽  
...  
Keyword(s):  
Antibody Response ◽  
Cell Survival ◽  
Plasma Cell ◽  
Specific Antibody ◽  
Transcriptome Profiling ◽  
Specific Antibody Response ◽  
Survival Factor ◽  
Whole Transcriptome ◽  
Cell Survival Factor

scholarly journals A highly specific antibody response after protein prime-peptide boost immunization with Eppin/B-cell epitope in mice

2011 ◽  
Vol 7 (8) ◽  
pp. 849-855 ◽  
Author(s):  
Zhengqiong Chen ◽  
Wei He ◽  
Yuzhang Wu ◽  
Ping Yan ◽  
Haiyang He ◽  
...  
Keyword(s):  
Antibody Response ◽  
B Cell ◽  
Specific Antibody ◽  
Cell Epitope ◽  
Specific Antibody Response ◽  
B Cell Epitope ◽  
Boost Immunization

Assessment of the antigen-specific antibody response induced by mucosal administration of a GnRH conjugate entrapped in lipid nanoparticles

2014 ◽  
Vol 10 (5) ◽  
pp. e971-e979 ◽  
Author(s):  
Ayman M. Gebril ◽  
Dimitrios A. Lamprou ◽  
Manal M. Alsaadi ◽  
William H. Stimson ◽  
Alexander B. Mullen ◽  
...  
Keyword(s):  
Antibody Response ◽  
Specific Antibody ◽  
Lipid Nanoparticles ◽  
Specific Antibody Response

Characterization of Donor Specific Antibody Response Following Liver Transplantation in Rats.

2014 ◽  
Vol 98 ◽  
pp. 386-387
Author(s):  
Y. Xie ◽  
Y. Wu ◽  
J. Wang ◽  
J. Ye ◽  
J. Levitsky ◽  
...  
Keyword(s):  
Liver Transplantation ◽  
Antibody Response ◽  
Specific Antibody ◽  
Specific Antibody Response

scholarly journals Poor specific antibody response in patients with mucopolysaccaridosis

2019 ◽  
Vol 143 (2) ◽  
pp. AB15
Author(s):  
Marilia M. Moraes ◽  
Isabella B. Manhaes ◽  
Luiza M. Marino ◽  
Julio Cesar Gontijo ◽  
Barbara Luiza B. Cancado ◽  
...  
Keyword(s):  
Antibody Response ◽  
Specific Antibody ◽  
Specific Antibody Response

scholarly journals Anti-factor VIII antibodies of hemophiliac patients are frequently directed towards nonfunctional determinants and do not exhibit isotypic restriction

Blood ◽  
1993 ◽  
Vol 82 (8) ◽  
pp. 2452-2461 ◽  
Author(s):  
JG Gilles ◽  
J Arnout ◽  
J Vermylen ◽  
JM Saint-Remy
Keyword(s):  
Antibody Response ◽  
Factor Viii ◽  
Specific Antibody ◽  
Hemophilia A ◽  
Significant Proportion ◽  
Functional Method ◽  
Specific Antibody Response ◽  
Functional Assays ◽  
Epitope Specificity ◽  
Chromogenic Assay

Abstract A significant proportion of hemophilia A patients receiving transfusions of factor VIII (FVIII) develop a specific antibody response towards FVIII. These antibodies are usually detected by assays in which they inhibit the function of the molecule, such as the Bethesda clotting test. We have prepared anti-FVIII antibodies by specific immunoadsorption from the plasma of four hemophiliacs with stable inhibitor levels. The isotypic distribution of such antibodies was determined and their capacity to bind to insolubilized FVIII was compared with their inhibitory activity in two functional assays, namely, the Bethesda assay and a chromogenic assay. In addition, the FVIII epitope specificity was determined by competition with monoclonal antibodies for the binding to insolubilized FVIII. We show here that (1) anti-FVIII antibodies are not isotypically restricted; thus, a significant proportion of specific IgG2 was found; (2) antibodies are frequently directed towards epitopes of FVIII that are not directly involved in the function of the molecule and therefore escape detection in the Bethesda method or chromogenic assay; and (3) each patient shows a unique pattern of FVIII epitope recognition. We conclude that evaluation of anti-FVIII antibodies by a functional method does not provide an accurate evaluation of the specific antibody response. These findings have important implications for the comparison of the immunogenicity of FVIII molecules produced by different technologies and for the development of methods to control anti-FVIII antibody production.

Interferon activation status underlies higher antibody response to viral antigens in patients with systemic lupus erythematosus receiving no or light treatment

Rheumatology ◽  
2020 ◽  
Author(s):  
Albin Björk ◽  
Rui Da Silva Rodrigues ◽  
Elina Richardsdotter Andersson ◽  
Jorge I Ramírez Sepúlveda ◽  
Johannes Mofors ◽  
...  
Keyword(s):  
Antibody Response ◽  
Specific Antibody ◽  
Lupus Erythematosus ◽  
Serum Proteins ◽  
Light Treatment ◽  
Antibody Responses ◽  
Type I ◽  
Specific Antibody Response ◽  
Viral Antigens

Abstract Objectives Infections have been proposed as an environmental risk factor for autoimmune disease. Responses to microbial antigens may be studied in vivo during vaccination. We therefore followed patients with SLE and controls during split-virion influenza vaccination to quantify antibody responses against viral antigens and associated cellular and proteome parameters. Methods Blood samples and clinical data were collected from female patients with SLE with no or HCQ and/or low-dose prednisolone treatment (n = 29) and age- and sex-matched healthy controls (n = 17). Vaccine-specific antibody titres were measured by ELISA and IFN-induced gene expression in monocytes by quantitative PCR. Serum proteins were measured by proximity extension assay and disease-associated symptoms were followed by questionnaires. Results The vaccine-specific antibody response was significantly higher in patients compared with controls and titres of IgG targeting the viral proteins were higher in patients than controls at both 1 and 3 months after immunization. Clinical disease symptoms and autoantibody titres remained unchanged throughout the study. Notably, a positive pre-vaccination mRNA-based IFN score was associated with a significantly higher vaccine-specific antibody response and with a broader profile of autoantibody specificities. Screening of serum protein biomarkers revealed higher levels of IFN-regulated proteins in patients compared with controls and that levels of such proteins correlated with the vaccine-specific IgG response, with C-C motif chemokine ligand 3 exhibiting the strongest association. Conclusion Augmented antibody responses to viral antigens develop in patients with SLE on no or light treatment and associate with markers of type I IFN system activation at the RNA and protein levels.

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