Infectivity of Liposomally Encapsulated Nucleic Acids Isolated from EMC Virus and Scrapie-Infected Mouse Brain

Intervirology ◽  
1983 ◽  
Vol 20 (2-3) ◽  
pp. 164-168 ◽  
Author(s):  
Jane Manning ◽  
G.C. Millson
Keyword(s):  
Nucleic Acids ◽  
Infected Mouse ◽  
Mouse Brain ◽  
Emc Virus

Separation of Isospora (Toxoplasma) gondii cysts and cystozoites from mouse brain tissue by continuous density - gradient centrifugation

Parasitology ◽  
1981 ◽  
Vol 83 (1) ◽  
pp. 103-108 ◽  
Author(s):  
A. W. C. A. Cornelissen ◽  
J. P. Overdulve ◽  
J. M. Hoenderboom
Keyword(s):  
Toxoplasma Gondii ◽  
Brain Tissue ◽  
Density Gradient ◽  
Infected Mouse ◽  
Mouse Brain ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Percoll Gradient ◽  
Isolation And Purification ◽  
Reproducible Method

SUMMARYA simple, quick and reproducible method consisting of density-gradient centrifugation of homogenized infected mouse brain tissue on Percoll is described for the isolation and purification of cysts of Isospora (Toxoplasma) gondii. A 100% recovery of cysts, with 74·2% in a single fraction with a specific gravity of 1·056, was obtained by overlaying homogenates of infected mouse brains on a pre-formed Percoll gradient and centrifugation at low g forces. With this procedure recovery was independent of the age of the cysts. Titration of purified cystozoites showed there to be no loss of infectivity.

Metabolism of Pyruvate and α-Ketoglutarate in Virus-infected Mouse Brain

Nature ◽  
1957 ◽  
Vol 180 (4599) ◽  
pp. 1418-1419 ◽  
Author(s):  
P. L. BRADLEY
Keyword(s):  
Infected Mouse ◽  
Mouse Brain

The association between PrP and infectivity in scrapie and BSE infected mouse brain

1996 ◽  
Vol 141 (2) ◽  
pp. 275-289 ◽  
Author(s):  
R. A. Somerville ◽  
A. J. Dunn
Keyword(s):  
Infected Mouse ◽  
Mouse Brain
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