The association between PrP and infectivity in scrapie and BSE infected mouse brain

1996 ◽  
Vol 141 (2) ◽  
pp. 275-289 ◽  
Author(s):  
R. A. Somerville ◽  
A. J. Dunn
Keyword(s):  
Infected Mouse ◽  
Mouse Brain

Separation of Isospora (Toxoplasma) gondii cysts and cystozoites from mouse brain tissue by continuous density - gradient centrifugation

Parasitology ◽  
1981 ◽  
Vol 83 (1) ◽  
pp. 103-108 ◽  
Author(s):  
A. W. C. A. Cornelissen ◽  
J. P. Overdulve ◽  
J. M. Hoenderboom
Keyword(s):  
Toxoplasma Gondii ◽  
Brain Tissue ◽  
Density Gradient ◽  
Infected Mouse ◽  
Mouse Brain ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Percoll Gradient ◽  
Isolation And Purification ◽  
Reproducible Method

SUMMARYA simple, quick and reproducible method consisting of density-gradient centrifugation of homogenized infected mouse brain tissue on Percoll is described for the isolation and purification of cysts of Isospora (Toxoplasma) gondii. A 100% recovery of cysts, with 74·2% in a single fraction with a specific gravity of 1·056, was obtained by overlaying homogenates of infected mouse brains on a pre-formed Percoll gradient and centrifugation at low g forces. With this procedure recovery was independent of the age of the cysts. Titration of purified cystozoites showed there to be no loss of infectivity.

Metabolism of Pyruvate and α-Ketoglutarate in Virus-infected Mouse Brain

Nature ◽  
1957 ◽  
Vol 180 (4599) ◽  
pp. 1418-1419 ◽  
Author(s):  
P. L. BRADLEY
Keyword(s):  
Infected Mouse ◽  
Mouse Brain

Tioman virus infection in experimentally infected mouse brain and its association with apoptosis

2007 ◽  
Vol 143 (2) ◽  
pp. 140-146 ◽  
Author(s):  
Koon Chu Yaiw ◽  
Kien Chai Ong ◽  
Kaw Bing Chua ◽  
John Bingham ◽  
Linfa Wang ◽  
...  
Keyword(s):  
Virus Infection ◽  
Infected Mouse ◽  
Mouse Brain

Detection of Toscana Virus-Specific Immunoglobulins G and M by an Enzyme-Linked Immunosorbent Assay Based on Recombinant Viral Nucleoprotein

1999 ◽  
Vol 37 (6) ◽  
pp. 2010-2012 ◽  
Author(s):  
Maria Grazia Ciufolini ◽  
Cristiano Fiorentini ◽  
Paola di Bonito ◽  
Stefania Mochi ◽  
Colomba Giorgi
Keyword(s):  
Infected Mouse ◽  
Sensitivity And Specificity ◽  
Mouse Brain ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Toscana Virus ◽  
Human Sera

An enzyme-linked immunosorbent assay (ELISA) based on the recombinant Toscana virus nucleoprotein (rN) has been developed. Its sensitivity and specificity for the detection of virus-specific immunoglobulins G and M in human sera were similar to those of the ELISA that is based on an antigen extracted from infected mouse brain and that is routinely used for serodiagnosis.

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