Metabolism of Pyruvate and α-Ketoglutarate in Virus-infected Mouse Brain

Nature ◽  
1957 ◽  
Vol 180 (4599) ◽  
pp. 1418-1419 ◽  
Author(s):  
P. L. BRADLEY
Keyword(s):  
Infected Mouse ◽  
Mouse Brain

Separation of Isospora (Toxoplasma) gondii cysts and cystozoites from mouse brain tissue by continuous density - gradient centrifugation

Parasitology ◽  
1981 ◽  
Vol 83 (1) ◽  
pp. 103-108 ◽  
Author(s):  
A. W. C. A. Cornelissen ◽  
J. P. Overdulve ◽  
J. M. Hoenderboom
Keyword(s):  
Toxoplasma Gondii ◽  
Brain Tissue ◽  
Density Gradient ◽  
Infected Mouse ◽  
Mouse Brain ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Percoll Gradient ◽  
Isolation And Purification ◽  
Reproducible Method

SUMMARYA simple, quick and reproducible method consisting of density-gradient centrifugation of homogenized infected mouse brain tissue on Percoll is described for the isolation and purification of cysts of Isospora (Toxoplasma) gondii. A 100% recovery of cysts, with 74·2% in a single fraction with a specific gravity of 1·056, was obtained by overlaying homogenates of infected mouse brains on a pre-formed Percoll gradient and centrifugation at low g forces. With this procedure recovery was independent of the age of the cysts. Titration of purified cystozoites showed there to be no loss of infectivity.

The association between PrP and infectivity in scrapie and BSE infected mouse brain

1996 ◽  
Vol 141 (2) ◽  
pp. 275-289 ◽  
Author(s):  
R. A. Somerville ◽  
A. J. Dunn
Keyword(s):  
Infected Mouse ◽  
Mouse Brain

Tioman virus infection in experimentally infected mouse brain and its association with apoptosis

2007 ◽  
Vol 143 (2) ◽  
pp. 140-146 ◽  
Author(s):  
Koon Chu Yaiw ◽  
Kien Chai Ong ◽  
Kaw Bing Chua ◽  
John Bingham ◽  
Linfa Wang ◽  
...  
Keyword(s):  
Virus Infection ◽  
Infected Mouse ◽  
Mouse Brain

Detection of Toscana Virus-Specific Immunoglobulins G and M by an Enzyme-Linked Immunosorbent Assay Based on Recombinant Viral Nucleoprotein

1999 ◽  
Vol 37 (6) ◽  
pp. 2010-2012 ◽  
Author(s):  
Maria Grazia Ciufolini ◽  
Cristiano Fiorentini ◽  
Paola di Bonito ◽  
Stefania Mochi ◽  
Colomba Giorgi
Keyword(s):  
Infected Mouse ◽  
Sensitivity And Specificity ◽  
Mouse Brain ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Toscana Virus ◽  
Human Sera

An enzyme-linked immunosorbent assay (ELISA) based on the recombinant Toscana virus nucleoprotein (rN) has been developed. Its sensitivity and specificity for the detection of virus-specific immunoglobulins G and M in human sera were similar to those of the ELISA that is based on an antigen extracted from infected mouse brain and that is routinely used for serodiagnosis.

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