A Method to Assess Histamine-Induced Cyclic AMP Production in Isolated Gastric Mucosal Cells from Human Biopsies

Pharmacology ◽  
1991 ◽  
Vol 42 (6) ◽  
pp. 327-332 ◽  
Author(s):  
A. Sarem-Aslani ◽  
D. Ratge ◽  
H.-H. Bigge ◽  
S. Walker ◽  
U. Klotz ◽  
...  
Keyword(s):  
Cyclic Amp ◽  
Gastric Mucosal Cells ◽  
Mucosal Cells

Actions of histamine, secretin, and PGE2 on cyclic AMP production by isolated canine fundic mucosal cells.

1979 ◽  
Vol 237 (5) ◽  
pp. E437
Author(s):  
A Wollin ◽  
A H Soll ◽  
I M Samloff
Keyword(s):  
Cyclic Amp ◽  
Parietal Cell ◽  
Cell Type ◽  
Gastric Mucosal Cells ◽  
Cell Content ◽  
Histamine Stimulation ◽  
Mucosal Cells ◽  
Single Cell Type ◽  
Cell Fractions ◽  
Stimulation Of

Cyclic AMP production was studied in isolated canine fundic gastric mucosal cells. Histamine, prostaglandin E2 (PGE2), and secretin increased cyclic AMP production by unenriched mucosal cells. In separated cell fractions, histamine stimulation of cyclic AMP production correlated with the parietal cell content of the fractions. Secretin in concentrations above 1 nM stimulated cyclic AMP production, and this effect correlated with the pepsinogen content of the separated cell fractions. At concentrations above 1 microM, PGE2 stimulated cyclic AMP production; this effect was found in all separated cell fractions and was not associated with any of the available cell markers. PGE2 stimulation of cyclic AMP production was, however, negatively correlated with the parietal cell content. Thus, histamine stimulated cyclic AMP production by parietal cells and secretin stimulated production of cyclic AMP by chief cells. PGE2 stimulation of cyclic AMP production could not be localized to a single cell type but occurred primarily in nonparietal cells.

In vitro Action of Gastric Mucosal Lysosomal Enzymes on Intracellular Gastric Glycoproteins

1979 ◽  
Vol 34 (1-2) ◽  
pp. 90-95 ◽  
Author(s):  
Fouad M. Fouad ◽  
D. Waldron-Edward
Keyword(s):  
Cell Wall ◽  
Lysosomal Enzymes ◽  
Gastric Ulceration ◽  
Mucosal Cell ◽  
Gastric Lesions ◽  
Acid Hydrolases ◽  
Gastric Mucosal Cells ◽  
Mucosal Cells ◽  
Specific Increase

Abstract The results show that incubation of gastric mucosal cells from rat at pH ~4.5 or in the presence of aspirin is associated with a specific increase in the activity of some acid-hydrolases. Intracellular glycoproteins, isolated by non-degradative techniques from rat or dog fundic mucosal cells, were found to be potential bio-substrates for these acid-hydrolyses. This may suggest that cleavage of the carbohydrate moieties of the intracellular and mucosal cell wall glycoproteins is a fundamental step in the development of gastric ulceration. A model for gastric lesions is proposed and discussed in the light of the results obtained.

Establishment of primary epithelial cell culture from elutriated rat gastric mucosal cells

1995 ◽  
Vol 30 (2) ◽  
pp. 135-141 ◽  
Author(s):  
Yoshikazu Kinoshita ◽  
Sazzad Hassan ◽  
Hirohisa Nakata ◽  
Masakyo Asahara ◽  
Yumi Matsushima ◽  
...  
Keyword(s):  
Cell Culture ◽  
Epithelial Cell ◽  
Epithelial Cell Culture ◽  
Gastric Mucosal Cells ◽  
Primary Epithelial Cell ◽  
Mucosal Cells
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