Interactions between intracellular cyclic AMP and agonist-induced inositol phospholipid breakdown in isolated gastric mucosal cells of the rat

1987 ◽  
Vol 336 (5) ◽  
Author(s):  
Juhani Puurunen ◽  
MartinJ. Lohse ◽  
Ulrich Schwabe
Keyword(s):  
Cyclic Amp ◽  
Gastric Mucosal Cells ◽  
Mucosal Cells ◽  
Inositol Phospholipid ◽  
Inositol Phospholipid Breakdown

A Method to Assess Histamine-Induced Cyclic AMP Production in Isolated Gastric Mucosal Cells from Human Biopsies

Pharmacology ◽  
1991 ◽  
Vol 42 (6) ◽  
pp. 327-332 ◽  
Author(s):  
A. Sarem-Aslani ◽  
D. Ratge ◽  
H.-H. Bigge ◽  
S. Walker ◽  
U. Klotz ◽  
...  
Keyword(s):  
Cyclic Amp ◽  
Gastric Mucosal Cells ◽  
Mucosal Cells

scholarly journals Requirement and role of arachidonic acid in the differentiation of pre-adipose cells

1989 ◽  
Vol 257 (2) ◽  
pp. 389-397 ◽  
Author(s):  
D Gaillard ◽  
R Négrel ◽  
M Lagarde ◽  
G Ailhaud
Keyword(s):  
Growth Hormone ◽  
Arachidonic Acid ◽  
Cyclic Amp ◽  
Adipose Conversion ◽  
Inositol Phospholipid ◽  
Glycerol 3 Phosphate Dehydrogenase ◽  
Triacylglycerol Accumulation ◽  
Exogenous Origin ◽  
Inositol Phospholipid Breakdown ◽  
Adipose Cells

The terminal adipose differentiation of Ob1771 cells, characterized by glycerol-3-phosphate dehydrogenase activity and triacylglycerol accumulation, was studied in serum-free hormone-supplemented medium containing growth hormone, tri-iodothyronine, insulin, transferrin and fetuin. Arachidonic acid was able to substitute for a crude adipogenic fraction isolated from fetal bovine serum but not for growth hormone or tri-iodothyronine. Arachidonic acid was also able to increase in a rapid and dramatic manner cyclic AMP production; moreover it was able to amplify the adipose conversion promoted by other agents elevating cyclic AMP concentrations and to induce inositol phospholipid breakdown. Both phorbol 12-myristate 13-acetate, a protein kinase C activator and ionomycin, a Ca2+-mobilizing agent, showed potent synergy with agents elevating cyclic AMP concentrations for the promotion of adipose conversion, whereas 8-bromo cyclic GMP and 4 alpha-phorbol 12,13-dibutyrate were ineffective. The triggering of both the cyclic AMP and inositol phospholipid pathways was accompanied by a single round of cell division, and within a few days all the cells became differentiated. Similar results were obtained, after exposure to arachidonic acid, with preadipose 3T3-F442A cells and with rat adipose precursor cells in primary culture. The availability of arachidonic acid from intracellular stores and/or of exogenous origin should play a major role for the onset of critical mitoses leading to terminal differentiation in pre-adipose cells.

Actions of histamine, secretin, and PGE2 on cyclic AMP production by isolated canine fundic mucosal cells.

1979 ◽  
Vol 237 (5) ◽  
pp. E437
Author(s):  
A Wollin ◽  
A H Soll ◽  
I M Samloff
Keyword(s):  
Cyclic Amp ◽  
Parietal Cell ◽  
Cell Type ◽  
Gastric Mucosal Cells ◽  
Cell Content ◽  
Histamine Stimulation ◽  
Mucosal Cells ◽  
Single Cell Type ◽  
Cell Fractions ◽  
Stimulation Of

Cyclic AMP production was studied in isolated canine fundic gastric mucosal cells. Histamine, prostaglandin E2 (PGE2), and secretin increased cyclic AMP production by unenriched mucosal cells. In separated cell fractions, histamine stimulation of cyclic AMP production correlated with the parietal cell content of the fractions. Secretin in concentrations above 1 nM stimulated cyclic AMP production, and this effect correlated with the pepsinogen content of the separated cell fractions. At concentrations above 1 microM, PGE2 stimulated cyclic AMP production; this effect was found in all separated cell fractions and was not associated with any of the available cell markers. PGE2 stimulation of cyclic AMP production was, however, negatively correlated with the parietal cell content. Thus, histamine stimulated cyclic AMP production by parietal cells and secretin stimulated production of cyclic AMP by chief cells. PGE2 stimulation of cyclic AMP production could not be localized to a single cell type but occurred primarily in nonparietal cells.

In vitro Action of Gastric Mucosal Lysosomal Enzymes on Intracellular Gastric Glycoproteins

1979 ◽  
Vol 34 (1-2) ◽  
pp. 90-95 ◽  
Author(s):  
Fouad M. Fouad ◽  
D. Waldron-Edward
Keyword(s):  
Cell Wall ◽  
Lysosomal Enzymes ◽  
Gastric Ulceration ◽  
Mucosal Cell ◽  
Gastric Lesions ◽  
Acid Hydrolases ◽  
Gastric Mucosal Cells ◽  
Mucosal Cells ◽  
Specific Increase

Abstract The results show that incubation of gastric mucosal cells from rat at pH ~4.5 or in the presence of aspirin is associated with a specific increase in the activity of some acid-hydrolases. Intracellular glycoproteins, isolated by non-degradative techniques from rat or dog fundic mucosal cells, were found to be potential bio-substrates for these acid-hydrolyses. This may suggest that cleavage of the carbohydrate moieties of the intracellular and mucosal cell wall glycoproteins is a fundamental step in the development of gastric ulceration. A model for gastric lesions is proposed and discussed in the light of the results obtained.

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