Regional mapping of the human galactocerebrosidase gene (GALC) to 14q31 by in situ hybridization

1994 ◽  
Vol 66 (4) ◽  
pp. 244-245 ◽  
Author(s):  
L.A. Cannizzaro ◽  
Y.Q. Chen ◽  
M.A. Rafi ◽  
D.A. Wenger
Keyword(s):  
In Situ Hybridization ◽  
Regional Mapping

Regional mapping to 4q32.1 by in situ hybridization of a DNA domain rearranged in human liver cancer

1988 ◽  
Vol 80 (3) ◽  
pp. 274-276 ◽  
Author(s):  
Véronique Blanquet ◽  
Floriane Garreau ◽  
X. Chenivesse ◽  
C. Brechot ◽  
Catherine Turleau
Keyword(s):  
In Situ Hybridization ◽  
Liver Cancer ◽  
Human Liver ◽  
Regional Mapping ◽  
Human Liver Cancer

Regional mapping of the human gene for lysosomal ?-glucosidase by in situ hybridization

1984 ◽  
Vol 67 (3) ◽  
pp. 326-328 ◽  
Author(s):  
D.J.J. Halley ◽  
A. Konings ◽  
P. Hupkes ◽  
H. Galjaard
Keyword(s):  
In Situ Hybridization ◽  
Human Gene ◽  
Regional Mapping

Regional mapping of the human NSP gene to chromosome region 14q21→q22 by fluorescence in situ hybridization analysis

1994 ◽  
Vol 66 (1) ◽  
pp. 48-50 ◽  
Author(s):  
P.F.J. Kools ◽  
A.J.M. Roebroek ◽  
H.J.K. Van deVelde ◽  
P. Marynen ◽  
J. Bullerdiek ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Chromosome Region ◽  
Hybridization Analysis ◽  
Regional Mapping

Regional mapping of the human gene encoding the novel pituitary polypeptide 7B2 to chromosome 15q13→q14 by in situ hybridization

1989 ◽  
Vol 50 (2-3) ◽  
pp. 158-160 ◽  
Author(s):  
A.J.M. Roebroek ◽  
M.R.M. Dehaen ◽  
A. van Bokhoven ◽  
G.J.M. Martens ◽  
P. Marÿnen ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Human Gene ◽  
The Novel ◽  
Regional Mapping ◽  
Gene Encoding

DNA sequence mapping in interphase and metaphase chromosomes by fluorescence in situ hybridization

1992 ◽  
Vol 50 (1) ◽  
pp. 496-497
Author(s):  
Barbara Trask ◽  
Susan Allen ◽  
Anne Bergmann ◽  
Mari Christensen ◽  
Anne Fertitta ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Dna Sequence ◽  
Dna Sequences ◽  
Dual Band ◽  
Nick Translation ◽  
Metaphase Chromosomes ◽  
Band Pass ◽  
Texas Red ◽  
Fluorescent Spot

Using fluorescence in situ hybridization (FISH), the positions of DNA sequences can be discretely marked with a fluorescent spot. The efficiency of marking DNA sequences of the size cloned in cosmids is 90-95%, and the fluorescent spots produced after FISH are ≈0.3 μm in diameter. Sites of two sequences can be distinguished using two-color FISH. Different reporter molecules, such as biotin or digoxigenin, are incorporated into DNA sequence probes by nick translation. These reporter molecules are labeled after hybridization with different fluorochromes, e.g., FITC and Texas Red. The development of dual band pass filters (Chromatechnology) allows these fluorochromes to be photographed simultaneously without registration shift.

Ultrastructural analysis of the spatial distribution of MRNA

1992 ◽  
Vol 50 (1) ◽  
pp. 552-553
Author(s):  
Gary Bassell ◽  
Robert H. Singer
Keyword(s):  
Electron Microscopy ◽  
Spatial Distribution ◽  
In Situ Hybridization ◽  
High Resolution ◽  
Nucleic Acids ◽  
Colloidal Gold ◽  
Dna Probe ◽  
Nucleotide Analogs ◽  
Gold Particles

We have been investigating the spatial distribution of nucleic acids intracellularly using in situ hybridization. The use of non-isotopic nucleotide analogs incorporated into the DNA probe allows the detection of the probe at its site of hybridization within the cell. This approach therefore is compatible with the high resolution available by electron microscopy. Biotinated or digoxigenated probe can be detected by antibodies conjugated to colloidal gold. Because mRNA serves as a template for the probe fragments, the colloidal gold particles are detected as arrays which allow it to be unequivocally distinguished from background.

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