Regional mapping of cKi-rasproto-oncogene on mouse chromosome 6 by in situ hybridization

1985 ◽  
Vol 39 (2) ◽  
pp. 140-144 ◽  
Author(s):  
L.A. Cahilly ◽  
D.L. George
Keyword(s):  
In Situ Hybridization ◽  
Mouse Chromosome ◽  
Chromosome 6 ◽  
Regional Mapping

Assignment1 of the centrosomal protein 110 gene (Cep110) to mouse chromosome bands 2B–C1 by in situ hybridization

2000 ◽  
Vol 89 (3-4) ◽  
pp. 216-217
Author(s):  
C. Popovici ◽  
M.-G. Mattéi ◽  
J.B. Rattner ◽  
D. Birnbaum ◽  
M.-J. Pébusque
Keyword(s):  
In Situ Hybridization ◽  
Mouse Chromosome ◽  
Centrosomal Protein ◽  
Chromosome Bands

Recovery of a telomere-truncated chromosome via a compensating translocation in maize

Genome ◽  
2011 ◽  
Vol 54 (3) ◽  
pp. 184-195 ◽  
Author(s):  
Robert T. Gaeta ◽  
Tatiana V. Danilova ◽  
Changzeng Zhao ◽  
Rick E. Masonbrink ◽  
Morgan E. McCaw ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Transgene Expression ◽  
De Novo ◽  
Extra Chromosome ◽  
Single Gene ◽  
B Chromosomes ◽  
Chromosome 1 ◽  
Chromosome 6

Maize-engineered minichromosomes are easily recovered from telomere-truncated B chromosomes but are rarely recovered from A chromosomes. B chromosomes lack known genes, and their truncation products are tolerated and transmitted during meiosis. In contrast, deficiency gametes resulting from truncated A chromosomes prevent their transmission. We report here a de novo compensating translocation that permitted recovery of a large truncation of chromosome 1 in maize. The truncation (trunc-1) and translocation with chromosome 6 (super-6) occurred during telomere-mediated truncation experiments and were characterized using single-gene fluorescent in situ hybridization (FISH) probes. The truncation contained a transgene signal near the end of the broken chromosome and transmitted together with the compensating translocation as a heterozygote to approximately 41%–55% of progeny. Transmission as an addition chromosome occurred in ~15% of progeny. Neither chromosome transmitted through pollen. Transgene expression (Bar) cosegregated with trunc-1 transcriptionally and phenotypically. Meiosis in T1 plants revealed eight bivalents and one tetravalent chain composed of chromosome 1, trunc-1, chromosome 6, and super-6 in diplotene and diakinesis. Our data suggest that de novo compensating translocations allow recovery of truncated A chromosomes by compensating deficiency in female gametes and by affecting chromosome pairing and segregation. The truncated chromosome can be maintained as an extra chromosome or together with the super-6 as a heterozygote.

Assignment1 of the mouse Vegfb gene to mouse chromosome 19 B by in situ hybridization

2001 ◽  
Vol 95 (3-4) ◽  
pp. 242-243
Author(s):  
L. Gerace ◽  
N. Cirenei ◽  
M. Cappelletti ◽  
R. Petraroli ◽  
F. Sebastiani ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Mouse Chromosome ◽  
Chromosome 19 ◽  
I Gene
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