Double synchronization of human lymphocyte cultures: selection for high-resolution banded metaphases in the first and second division

1985 ◽  
Vol 39 (4) ◽  
pp. 292-295 ◽  
Author(s):  
M. Rønne
Keyword(s):  
High Resolution ◽  
Human Lymphocyte ◽  
Lymphocyte Cultures ◽  
Selection For

Synchronization of human lymphocyte cultures by fluorodeoxyuridine

1985 ◽  
Vol 27 (5) ◽  
pp. 622-625 ◽  
Author(s):  
M. De Braekeleer ◽  
Marylinne Keushnig ◽  
C. C. Lin
Keyword(s):  
High Resolution ◽  
Human Lymphocyte ◽  
Simple Technique ◽  
S Phase ◽  
Human Chromosomes ◽  
Hoechst 33258 ◽  
Simultaneous Exposure ◽  
Simple Method ◽  
Cell Synchronization ◽  
Lymphocyte Cultures

A simple technique for synchronization of human lymphocyte cultures with fluorodeoxyuridine (FudR) is presented. The S-phase block induced by the FudR is released by simultaneous exposure to 5-bromodeoxyuridine (BrdU) and Hoechst 33258 or by thymidine and Hoechst 33258. This method provides a high mitotic index with high percentage of prometaphase chromosomes. This simple method is highly advantageous and easy to utilize in clinical cytogenetics.Key words: fluorodeoxyuridine, high-resolution human chromosomes, cell synchronization, banding.

Mitotic arrest by benzimidazole analogs in human lymphocyte cultures

1980 ◽  
Vol 2 (1) ◽  
pp. 67-73 ◽  
Author(s):  
Henry E. Holden ◽  
Paula A. Crider ◽  
Margitta G. Wahrenburg
Keyword(s):  
Human Lymphocyte ◽  
Mitotic Arrest ◽  
Lymphocyte Cultures

Studies of the Producer Cell of Interferon in Human Lymphocyte Cultures

1979 ◽  
Vol 156 (1-2) ◽  
pp. 65-75
Author(s):  
H. Kirchner ◽  
H.H. Peter ◽  
H.M. Hirt ◽  
R. Zawatzky ◽  
H. Dalügge ◽  
...  
Keyword(s):  
Human Lymphocyte ◽  
Lymphocyte Cultures ◽  
Producer Cell

Effect of low molecular weight human serum factors and human somatomedin peptides on human lymphocyte cultures

1982 ◽  
Vol 57 (1) ◽  
pp. 129-137
Author(s):  
MH Heulin ◽  
M Artur ◽  
J Straczek ◽  
F Belleville ◽  
P Nabet ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Human Serum ◽  
Human Lymphocyte ◽  
Low Molecular Weight ◽  
Serum Factors ◽  
Lymphocyte Cultures
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