Synchronization of human lymphocyte cultures by fluorodeoxyuridine

1985 ◽  
Vol 27 (5) ◽  
pp. 622-625 ◽  
Author(s):  
M. De Braekeleer ◽  
Marylinne Keushnig ◽  
C. C. Lin
Keyword(s):  
High Resolution ◽  
Human Lymphocyte ◽  
Simple Technique ◽  
S Phase ◽  
Human Chromosomes ◽  
Hoechst 33258 ◽  
Simultaneous Exposure ◽  
Simple Method ◽  
Cell Synchronization ◽  
Lymphocyte Cultures

A simple technique for synchronization of human lymphocyte cultures with fluorodeoxyuridine (FudR) is presented. The S-phase block induced by the FudR is released by simultaneous exposure to 5-bromodeoxyuridine (BrdU) and Hoechst 33258 or by thymidine and Hoechst 33258. This method provides a high mitotic index with high percentage of prometaphase chromosomes. This simple method is highly advantageous and easy to utilize in clinical cytogenetics.Key words: fluorodeoxyuridine, high-resolution human chromosomes, cell synchronization, banding.

scholarly journals JunB Breakdown in Mid-/Late G2 Is Required for Down-Regulation of Cyclin A2 Levels and Proper Mitosis

2008 ◽  
Vol 28 (12) ◽  
pp. 4173-4187 ◽  
Author(s):  
Rosa Farràs ◽  
Véronique Baldin ◽  
Sandra Gallach ◽  
Claire Acquaviva ◽  
Guillaume Bossis ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Tumor Suppressor ◽  
Genetic Instability ◽  
S Phase ◽  
Subsequent Reduction ◽  
Suppressor Activity ◽  
Cell Synchronization ◽  
Tumor Suppressor Activity ◽  
A Cell ◽  
Cyclin A2

ABSTRACT JunB, a member of the AP-1 family of dimeric transcription factors, is best known as a cell proliferation inhibitor, a senescence inducer, and a tumor suppressor, although it also has been attributed a cell division-promoting activity. Its effects on the cell cycle have been studied mostly in G1 and S phases, whereas its role in G2 and M phases still is elusive. Using cell synchronization experiments, we show that JunB levels, which are high in S phase, drop during mid- to late G2 phase due to accelerated phosphorylation-dependent degradation by the proteasome. The forced expression of an ectopic JunB protein in late G2 phase indicates that JunB decay is necessary for the subsequent reduction of cyclin A2 levels in prometaphase, the latter event being essential for proper mitosis. Consistently, abnormal JunB expression in late G2 phase entails a variety of mitotic defects. As these aberrations may cause genetic instability, our findings contrast with the acknowledged tumor suppressor activity of JunB and reveal a mechanism by which the deregulation of JunB might contribute to tumorigenesis.

High resolution analysis and differential condensation in RBA-banded human chromosomes

1981 ◽  
Vol 59 (3) ◽  
Author(s):  
E. Schollmayer ◽  
D. Sch�fer ◽  
B. Frisch ◽  
E. Schleiermacher
Keyword(s):  
High Resolution ◽  
Human Chromosomes ◽  
High Resolution Analysis ◽  
Resolution Analysis
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