Replication pattern and quinacrine fluorescence of the chromosomes of a CV-1-derived African green monkey cell line

1973 ◽  
Vol 12 (2) ◽  
pp. 93-106 ◽  
Author(s):  
R.J. Parker ◽  
P.W. Allderdice ◽  
O.J. Miller ◽  
C.L. Schildkraut
Keyword(s):  
Cell Line ◽  
African Green Monkey ◽  
Replication Pattern ◽  
Monkey Cell ◽  
Green Monkey ◽  
Quinacrine Fluorescence

Molecular characterization of small polydisperse circular DNA from an African green monkey cell line

Biochemistry ◽  
1982 ◽  
Vol 21 (9) ◽  
pp. 2076-2085 ◽  
Author(s):  
Arthur H. Bertelsen ◽  
M. Zafri Humayun ◽  
Stefan G. Karfopoulos ◽  
Mark G. Rush
Keyword(s):  
Cell Line ◽  
Molecular Characterization ◽  
African Green Monkey ◽  
Circular Dna ◽  
Monkey Cell ◽  
Green Monkey

An African green monkey cell line RAMT resistant simultaneously to 8-azaguanine, 6-mercaptopurine, and 6-thioguanine

1982 ◽  
Vol 94 (4) ◽  
pp. 1449-1451
Author(s):  
V. I. Stobetskii ◽  
V. P. Grachev ◽  
L. L. Mironova ◽  
V. G. Chernikov
Keyword(s):  
Cell Line ◽  
African Green Monkey ◽  
Monkey Cell ◽  
Green Monkey

PLAQUE FORMATION BY POLIO AND MEASLES VIRUSES IN BS-C-1 (HOPPS) CELLS DERIVED FROM THE AFRICAN GREEN MONKEY

1965 ◽  
Vol 11 (3) ◽  
pp. 435-439 ◽  
Author(s):  
John Furesz ◽  
Pierre Moreau
Keyword(s):  
Cell Line ◽  
Measles Virus ◽  
Plaque Assay ◽  
Plaque Formation ◽  
African Green Monkey ◽  
High Passage ◽  
Green Monkey ◽  
Simple Medium ◽  
Low Passage ◽  
Passage Cells

BS-C-1 cells, cultivated in a simple medium designated M-E, were found to be suitable for a plaque assay of polio and measles viruses. In the plaque assay both low- and high-passage levels of the BS-C-1 cell line were highly susceptible to measles virus but titers of polioviruses were lower in the high-passage cells. Low-passage cells, preserved and stored at −196 °C for prolonged periods, are recommended for the titration of polio and measles viruses.

Baseline karyologic values for primary rhesus and African green monkey cell cultures

1974 ◽  
Vol 2 (4) ◽  
pp. 333-338 ◽  
Author(s):  
J.C. Petricciani ◽  
F.C. Chu ◽  
J.E. Cooper ◽  
T.C. Hsu
Keyword(s):  
Cell Cultures ◽  
African Green Monkey ◽  
Monkey Cell ◽  
Green Monkey

scholarly journals Cytotoxic and Genotoxic Effects of Fluconazole on African Green Monkey Kidney (Vero) Cell Line

2018 ◽  
Vol 2018 ◽  
pp. 1-7
Author(s):  
Regianne Maciel dos Santos Correa ◽  
Tatiane Cristina Mota ◽  
Adriana Costa Guimarães ◽  
Laís Teixeira Bonfim ◽  
Rommel Rodriguez Burbano ◽  
...  
Keyword(s):  
Comet Assay ◽  
Vero Cell ◽  
Cell Line ◽  
Vero Cells ◽  
Negative Control ◽  
African Green Monkey ◽  
Monkey Kidney ◽  
African Green Monkey Kidney ◽  
Vero Cell Line ◽  
Green Monkey

Fluconazole is a broad-spectrum triazole antifungal that is well-established as the first-line treatment for Candida albicans infections. Despite its extensive use, reports on its genotoxic/mutagenic effects are controversial; therefore, further studies are needed to better clarify such effects. African green monkey kidney (Vero) cells were exposed in vitro to different concentrations of fluconazole and were then evaluated for different parameters, such as cytotoxicity (MTT/cell death by fluorescent dyes), genotoxicity/mutagenicity (comet assay/micronucleus test), and induction of oxidative stress (DCFH-DA assay). Fluconazole was used at concentrations of 81.6, 163.2, 326.5, 653, 1306, and 2612.1μM for the MTT assay and 81.6, 326.5, and 1306μM for the remaining assays. MTT results showed that cell viability reduced upon exposure to fluconazole concentration of 1306μM (85.93%), being statistically significant (P<0.05) at fluconazole concentration of 2612.1μM (35.25%), as compared with the control (100%). Fluconazole also induced necrosis (P<0.05) in Vero cell line when cells were exposed to all concentrations (81.6, 326.5, and 1306μM) for both tested harvest times (24 and 48 h) as compared with the negative control. Regarding genotoxicity/mutagenicity, results showed fluconazole to increase significantly (P<0.05) DNA damage index, as assessed by comet assay, at 1306μM versus the negative control (DI=1.17 vs DI=0.28, respectively). Micronucleus frequency also increased until reaching statistical significance (P<0.05) at 1306μM fluconazole (with 42MN/1000 binucleated cells) as compared to the negative control (13MN/1000 binucleated cells). Finally, significant formation of reactive oxygen species (P<0.05) was observed at 1306μM fluconazole vs the negative control (OD=40.9 vs OD=32.3, respectively). Our experiments showed that fluconazole is cytotoxic and genotoxic in the assessed conditions. It is likely that such effects may be due to the oxidative properties of fluconazole and/or the presence of FMO (flavin-containing monooxygenase) in Vero cells.

scholarly journals Characterization of a novel embryonic stem cell line from an ICSI-derived blastocyst in the African green monkey

Reproduction ◽  
2010 ◽  
Vol 139 (3) ◽  
pp. 565-573 ◽  
Author(s):  
Nobuhiro Shimozawa ◽  
Shinichiro Nakamura ◽  
Ichiro Takahashi ◽  
Masanori Hatori ◽  
Tadashi Sankai
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Biomedical Research ◽  
Es Cells ◽  
Cell Monolayer ◽  
Embryonic Stem ◽  
African Green Monkey ◽  
Embryoid Bodies ◽  
Es Cell ◽  
Green Monkey

Several cell types from the African green monkey (Cercopithecus aethiops), such as red blood cells, primary culture cells from kidney, and the Vero cell line, are valuable sources for biomedical research and testing. Embryonic stem (ES) cells that are established from blastocysts have pluripotency to differentiate into these and other types of cells. We examined an in vitro culture system of zygotes produced by ICSI in African green monkeys and attempted to establish ES cells. Culturing with and without a mouse embryonic fibroblast (MEF) cell monolayer resulted in the development of ICSI-derived zygotes to the blastocyst stage, while culturing with a buffalo rat liver cell monolayer yielded no development (3/14, 21.4% and 6/31, 19.4% vs 0/23, 0% respectively; P<0.05). One of the nine blastocysts, which had been one of the zygotes co-cultured with MEF cells, formed flat colonies consisting of cells with large nuclei, similar to other primate ES cell lines. The African green monkey ES (AgMES) cells expressed pluripotency markers, formed teratomas consisting of three embryonic germ layer tissues, and had a normal chromosome number. Furthermore, expression of the germ cell markers CD9 and DPPA3 (STELLA) was detected in the embryoid bodies, suggesting that AgMES cells might have the potential ability to differentiate into germ cells. The results suggested that MEF cells greatly affected the quality of the inner cell mass of the blastocysts. In addition, AgMES cells would be a precious resource for biomedical research such as other primate ES cell lines.

Morphological characterisation of BGM (Buffalo Green Monkey) cell line exposed to low doses of cadmium chloride

2003 ◽  
Vol 17 (3) ◽  
pp. 293-299 ◽  
Author(s):  
D Romero ◽  
M Gómez-Zapata ◽  
A Luna ◽  
A.J Garcı́a-Fernández
Keyword(s):  
Cell Line ◽  
Cadmium Chloride ◽  
Low Doses ◽  
Morphological Characterisation ◽  
Monkey Cell ◽  
Green Monkey
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