Wheat IgE-Mediated Food Allergy in European Patients: α-Amylase Inhibitors, Lipid Transfer Proteins and Low-Molecular-Weight Glutenins

2007 ◽  
Vol 144 (1) ◽  
pp. 10-22 ◽  
Author(s):  
Elide A. Pastorello ◽  
Laura Farioli ◽  
Amedeo Conti ◽  
Valerio Pravettoni ◽  
Simona Bonomi ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Food Allergy ◽  
Low Molecular Weight ◽  
Lipid Transfer ◽  
Lipid Transfer Proteins ◽  
Ige Mediated ◽  
Amylase Inhibitors

scholarly journals Sensitisation to lipid transfer proteins in pollen – allergic adults with food allergy

2020 ◽  
Vol 37 (4) ◽  
pp. 508-512
Author(s):  
Adam Wawrzeńczyk ◽  
Magdalena Żbikowska-Gotz ◽  
Anna Wawrzenczyk ◽  
Zbigniew Bartuzi
Keyword(s):  
Food Allergy ◽  
Lipid Transfer ◽  
Lipid Transfer Proteins

scholarly journals Sustained Drug-related Reaction With Eosinophilia and Systemic Symptoms (DRESS) Triggered by Low Molecular Weight Heparins in COVID-19: Management and Precision Diagnosis.

2021 ◽  
Author(s):  
Elena Minenna ◽  
Nada Chaoul ◽  
Maria Pia Rossi ◽  
Lucia Giliberti ◽  
Marcello Albanesi ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Lymphocyte Proliferation ◽  
Low Molecular Weight ◽  
High Dose ◽  
Low Molecular Weight Heparins ◽  
First Case ◽  
Ige Mediated ◽  
Systemic Symptoms ◽  
Lymphocyte Proliferation Test ◽  
Related Reaction

Abstract Background. Low molecular weight heparins (LMWHs) are administered routinely to COVID-19 patients. Systemic reactions to LMWHs (both IgE-mediated and non IgE-mediated) are considered rare. Particularly, a single case of drug-related reaction with eosinophilia and systemic symptoms (DRESS) due to enoxaparin has been described in the literature. Here, we report on the first case of DRESS related to administration of fondaparinux.Case presentation. An elderly woman was diagnosed with COVID-19, during the “first wave” of the pandemics in Italy, and treated with enoxaparin (for anti-coagulation). A DRESS syndrome developed after two weeks (RegiSCAR score = 7). Enoxaparin was considered the culprit drug and replaced with the fully synthetic LMWH fondaparinux. At the same time, high-dosage prednisone was added to the therapy. The DRESS slowly subsided and the patient survived. Upon recovery and corticosteroid washout, a lymphocyte proliferation test for both LMWHs was performed, yielding a positive response not only for enoxaparin but also for fondaparinux. Conclusions. By lymphocyte proliferation test we demonstrated that both enoxaparin and fondaparinux were implicated in this case of DRESS, occurring in an elderly COVID-19 patient. In retrospect, the choice of using a sustained high-dose corticosteroid treatment while maintaining anti-coagulation by fondaparinux proved to be right. Ultimately, the patient recovered from both DRESS and COVID-19.

scholarly journals Identification and Purification of Novel Low-Molecular-Weight Lupine Allergens as Components for Personalized Diagnostics

Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 409
Author(s):  
Uta Jappe ◽  
Arabella Karstedt ◽  
Daniela Warneke ◽  
Saskia Hellmig ◽  
Marisa Böttger ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Expressed Sequence Tag ◽  
Lipid Transfer Protein ◽  
Extraction Methods ◽  
Low Molecular Weight ◽  
Lipid Transfer ◽  
Prick Test ◽  
Ige Reactivity ◽  
Potential Marker ◽  
Marker Allergens

Lupine flour is a valuable food due to its favorable nutritional properties. In spite of its allergenic potential, its use is increasing. Three lupine species, Lupinus angustifolius, L. luteus, and L. albus are relevant for human nutrition. The aim of this study is to clarify whether the species differ with regard to their allergen composition and whether anaphylaxis marker allergens could be identified in lupine. Patients with the following characteristics were included: lupine allergy, suspected lupine allergy, lupine sensitization only, and peanut allergy. Lupine sensitization was detected via CAP-FEIA (ImmunoCAP) and skin prick test. Protein, DNA and expressed sequence tag (EST) databases were queried for lupine proteins homologous to already known legume allergens. Different extraction methods applied on seeds from all species were examined by SDS-PAGE and screened by immunoblotting for IgE-binding proteins. The extracts underwent different and successive chromatography methods. Low-molecular-weight components were purified and investigated for IgE-reactivity. Proteomics revealed a molecular diversity of the three species, which was confirmed when investigated for IgE-reactivity. Three new allergens, L. albus profilin, L. angustifolius and L. luteus lipid transfer protein (LTP), were identified. LTP as a potential marker allergen for severity is a valuable additional candidate for molecular allergy diagnostic tests.

Clinical role of lipid transfer proteins in food allergy

2004 ◽  
Vol 48 (5) ◽  
pp. 356-362 ◽  
Author(s):  
Elide A. Pastorello ◽  
Anna M. Robino
Keyword(s):  
Food Allergy ◽  
Lipid Transfer ◽  
Lipid Transfer Proteins ◽  
Clinical Role

Involvement of Lipid Transfer Proteins in Food Allergy to Wheat

2011 ◽  
pp. 383-386 ◽  
Author(s):  
F. Battais ◽  
J.P Douliez ◽  
D. Marion ◽  
Y. Popineau ◽  
G. Kanny ◽  
...  
Keyword(s):  
Food Allergy ◽  
Lipid Transfer ◽  
Lipid Transfer Proteins

A high-performance oil-free backing pump for electron microscopes

1978 ◽  
Vol 36 (1) ◽  
pp. 110-111
Author(s):  
G.K.W. Balkau ◽  
E. Bez ◽  
J.L. Farrant
Keyword(s):  
Molecular Weight ◽  
Electron Microscope ◽  
Hot Spots ◽  
High Performance ◽  
Carbonaceous Material ◽  
Contamination Rate ◽  
Low Molecular Weight ◽  
Principal Source ◽  
Rotary Pumps ◽  
Electron Microscopes

The earliest account of the contamination of electron microscope specimens by the deposition of carbonaceous material during electron irradiation was published in 1947 by Watson who was then working in Canada. It was soon established that this carbonaceous material is formed from organic vapours, and it is now recognized that the principal source is the oil-sealed rotary pumps which provide the backing vacuum. It has been shown that the organic vapours consist of low molecular weight fragments of oil molecules which have been degraded at hot spots produced by friction between the vanes and the surfaces on which they slide. As satisfactory oil-free pumps are unavailable, it is standard electron microscope practice to reduce the partial pressure of organic vapours in the microscope in the vicinity of the specimen by using liquid-nitrogen cooled anti-contamination devices. Traps of this type are sufficient to reduce the contamination rate to about 0.1 Å per min, which is tolerable for many investigations.

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