scholarly journals Identification and Purification of Novel Low-Molecular-Weight Lupine Allergens as Components for Personalized Diagnostics

Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 409
Author(s):  
Uta Jappe ◽  
Arabella Karstedt ◽  
Daniela Warneke ◽  
Saskia Hellmig ◽  
Marisa Böttger ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Expressed Sequence Tag ◽  
Lipid Transfer Protein ◽  
Extraction Methods ◽  
Low Molecular Weight ◽  
Lipid Transfer ◽  
Prick Test ◽  
Ige Reactivity ◽  
Potential Marker ◽  
Marker Allergens

Lupine flour is a valuable food due to its favorable nutritional properties. In spite of its allergenic potential, its use is increasing. Three lupine species, Lupinus angustifolius, L. luteus, and L. albus are relevant for human nutrition. The aim of this study is to clarify whether the species differ with regard to their allergen composition and whether anaphylaxis marker allergens could be identified in lupine. Patients with the following characteristics were included: lupine allergy, suspected lupine allergy, lupine sensitization only, and peanut allergy. Lupine sensitization was detected via CAP-FEIA (ImmunoCAP) and skin prick test. Protein, DNA and expressed sequence tag (EST) databases were queried for lupine proteins homologous to already known legume allergens. Different extraction methods applied on seeds from all species were examined by SDS-PAGE and screened by immunoblotting for IgE-binding proteins. The extracts underwent different and successive chromatography methods. Low-molecular-weight components were purified and investigated for IgE-reactivity. Proteomics revealed a molecular diversity of the three species, which was confirmed when investigated for IgE-reactivity. Three new allergens, L. albus profilin, L. angustifolius and L. luteus lipid transfer protein (LTP), were identified. LTP as a potential marker allergen for severity is a valuable additional candidate for molecular allergy diagnostic tests.

Recombinant Proteins and Peptides as Tools for Studying IgE Reactivity with Low-Molecular-Weight Glutenin Subunits in Some Wheat Allergies

2007 ◽  
Vol 55 (24) ◽  
pp. 9837-9845 ◽  
Author(s):  
Jacques Snégaroff ◽  
Gérard Branlard ◽  
Isabelle Bouchez-Mahiout ◽  
Béatrice Laudet ◽  
Martina Tylichova ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Recombinant Proteins ◽  
Low Molecular Weight ◽  
Glutenin Subunits ◽  
Ige Reactivity ◽  
Proteins And Peptides

Wheat IgE-Mediated Food Allergy in European Patients: α-Amylase Inhibitors, Lipid Transfer Proteins and Low-Molecular-Weight Glutenins

2007 ◽  
Vol 144 (1) ◽  
pp. 10-22 ◽  
Author(s):  
Elide A. Pastorello ◽  
Laura Farioli ◽  
Amedeo Conti ◽  
Valerio Pravettoni ◽  
Simona Bonomi ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Food Allergy ◽  
Low Molecular Weight ◽  
Lipid Transfer ◽  
Lipid Transfer Proteins ◽  
Ige Mediated ◽  
Amylase Inhibitors

Detection of Wheat Lipid Transfer Protein (Tri a 14) Sensitization: Comparison between Currently Available Diagnostic Tools

2021 ◽  
pp. 1-5
Author(s):  
Clara San Bartolomé ◽  
Rosa Muñoz-Cano ◽  
Josefina Rius ◽  
Rocío Casas-Saucedo ◽  
Sara Balsells ◽  
...  
Keyword(s):  
Lipid Transfer Protein ◽  
Poor Performance ◽  
Specific Ige ◽  
Diagnostic Tools ◽  
Lipid Transfer ◽  
Prick Test ◽  
Transfer Protein ◽  
Whole Wheat ◽  
Systemic Reactions ◽  
Routine Diagnosis

<b><i>Background:</i></b> Wheat lipid transfer protein (LTP; Tri a 14) and ω5-gliadin have been described as major allergens in wheat allergy (WA) and relevant in wheat-induced anaphylaxis, frequently associated with cofactors. <b><i>Objective:</i></b> The objective of this study was to compare tools currently available in routine diagnosis to detect Tri a 14 sensitization, its clinical relevance, and cosensitization to ω5-gliadin and other LTPs. <b><i>Methods:</i></b> One hundred eighteen adults sensitized to rTri a 14 by ImmunoCAP<sup>®</sup> (cutoff ≥0.1 kU<sub>A</sub>/L) identified among 210 LTP allergic patients were included. We evaluated (1) wheat skin prick test (SPT), (2) specific IgE (sIgE) to wheat, rTri a 14, rTri a 19, peach, apple, walnut, hazelnut, and peanut LTPs using ImmunoCAP<sup>®</sup> and microarray ImmunoCAP®ISAC (cutoff ≥0.3I SU), and (3) wheat-related symptoms. <b><i>Results:</i></b> Wheat SPT and sIgE were positive in 31% and 85% of subjects, respectively. rTri a 14 by microarray was detected in 25%. Eight percent showed cosensitization to ω5-gliadin. Thirty percent referred symptoms (gastrointestinal [13%], urticaria [11%], and anaphylaxis [8%]). Cofactors (45%) were significantly associated with systemic reactions. <b><i>Conclusion:</i></b> WA due to Tri a 14 is frequently related with systemic reactions and because are frequently related to cofactors, the culprit may not be suspected. Together with the poor performance to identify Tri a 14 sensitization of the current routine diagnostic tools based on the analysis of whole wheat extract, such as wheat SPT or sIgE, there is a high risk that WA may be overlooked. Thus, when WA is suspected, sIgE Tri a 14 assessment is recommended, together with wheat and ω5-gliadin, preferably in the singleplex format, and carefully evaluated considering ≥0.1 kUA/L as a cutoff.

scholarly journals GsLTP, a Non-Specific Lipid Transfer Protein from Glycine soja, Enhances Drought and Salt Stress Tolerance in Transgenic Tobacco

2020 ◽  
Author(s):  
Jinping Fan ◽  
Jingshuang Liu ◽  
Lei Cao ◽  
Jiayue Wang ◽  
Qingxue Meng ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Expressed Sequence Tag ◽  
Lipid Transfer Protein ◽  
Glycine Soja ◽  
Plant Responses ◽  
Lipid Transfer ◽  
Reading Frame ◽  
Microarray Expression

Abstract Background: Lipid transfer proteins (LTPs) mainly exist in plant cell walls where they make an important impact in the transport of diverse lipophilic compounds. They have an important effect on the stress tolerance of plants by mediating plant responses to environmental stimuli and cell signal transduction pathways. Although the functions of several LTPS in different plant species have been identified, little is known about the biochemical and enzymatic activities of LTP family members in soybeans.Results: Herein, GsLTP was identified from Glycine soja using soybean gene microarray expression and screened in a salt stress expressed sequence tag (EST) library. The 369 bp open reading frame (ORF) encodes a protein of 122 amino acids. The cDNA sequence shares similarity with LTP genes in other plants such as soybean (93%) and Vigna (72%). The function of the gene was characterized in transgenic tobacco. Various physiological characteristics under different environmental stresses were investigated. We discovered that overexpression of GsLTP enhanced tolerance to drought and salt stresses.Conclusions: Our results indicate that GsLTP plays an important role in multiple abiotic signalling pathways, and provide a theoretical basis and practical gene resource for crop breeding.

The effect of thermal processing on the IgE reactivity of the non-specific lipid transfer protein from apple, Mal d 3

Allergy ◽  
2005 ◽  
Vol 60 (10) ◽  
pp. 1262-1268 ◽  
Author(s):  
A. I. Sancho ◽  
N. M. Rigby ◽  
L. Zuidmeer ◽  
R. Asero ◽  
G. Mistrello ◽  
...  
Keyword(s):  
Thermal Processing ◽  
Lipid Transfer Protein ◽  
Lipid Transfer ◽  
Transfer Protein ◽  
Ige Reactivity ◽  
Specific Lipid

scholarly journals GsLTP, a Non-Specific Lipid Transfer Protein from Glycine soja, Enhances Drought and Salt Stress Tolerance in Transgenic Tobacco

2021 ◽  
Author(s):  
Jinping Fan ◽  
Jingshuang Liu ◽  
Lei Cao ◽  
Jiayue Wang ◽  
Qingxue Meng ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Expressed Sequence Tag ◽  
Lipid Transfer Protein ◽  
Glycine Soja ◽  
Plant Responses ◽  
Lipid Transfer ◽  
Reading Frame ◽  
Microarray Expression

Abstract Background: Lipid transfer proteins (LTPs) mainly exist in plant cell walls where they make an important impact in the transport of diverse lipophilic compounds. They have an important effect on the stress tolerance of plants by mediating plant responses to environmental stimuli and cell signal transduction pathways. Although the functions of several LTPs in different plant species have been identified, little is known about the biochemical and enzymatic activities of LTP family members in soybeans. Results: Herein, GsLTP was identified from Glycine soja using soybean gene microarray expression and screened in a salt stress expressed sequence tag (EST) library. The 369 bp open reading frame (ORF) encodes a protein of 122 amino acids. The cDNA sequence shares similarity with LTP genes in other plants such as soybean (93%) and Vigna (72%). The function of the gene was characterized in transgenic tobacco. Various physiological characteristics under different environmental stresses were investigated. We discovered that overexpression of GsLTP enhanced tolerance to drought and salt stresses. Conclusions: Our results indicate that GsLTP plays an important role in multiple abiotic signalling pathways, and provide a theoretical basis and practical gene resource for crop breeding.

scholarly journals GsLTP, a Non-Specific Lipid Transfer Protein from Glycine soja, Enhances Drought and Salt Stress Tolerance in Transgenic Tobacco

2020 ◽  
Author(s):  
Jinping Fan ◽  
Jingshuang Liu ◽  
Lei Cao ◽  
Jiayue Wang ◽  
Qingxue Meng ◽  
...  
Keyword(s):  
Salt Stress ◽  
Transgenic Tobacco ◽  
Expressed Sequence Tag ◽  
Lipid Transfer Protein ◽  
Glycine Soja ◽  
Lipid Transfer ◽  
Lipophilic Compounds ◽  
Reading Frame ◽  
Drought And Salt Stresses ◽  
Microarray Expression

Abstract Background Lipid transfer proteins (LTPs) mainly exist in plant cell walls where they make an important impact in the transport of diverse lipophilic compounds. Although the functions of several LTPS in different plant species have been identified, little is known about the biochemical and enzymatic activities of LTP family members in soybeans. Results Herein, GsLTP was identified from Glycine soja using soybean gene microarray expression and screened in a salt stress expressed sequence tag (EST) library. The 369 bp open reading frame (ORF) encodes a protein of 122 amino acids, and the cDNA sequence shares similarity with LTP genes in other plants such as soybean (93%) and Vigna (72%). The function of the gene was characterized in transgenic tobacco. Various physiological characteristics under different environmental stresses were investigated. We discovered that over-expression of GsLTP enhanced tolerance to drought and salt stresses. Conclusions Our results indicate that GsLTP plays an important role in multiple abiotic signalling pathways, and provide a theoretical basis and practical gene resource for crop breeding.

scholarly journals Beer, Cider, and Wine Allergy

2017 ◽  
Vol 2017 ◽  
pp. 1-4 ◽  
Author(s):  
Rhea A. Bansal ◽  
Susan Tadros ◽  
Amolak S. Bansal
Keyword(s):  
Lipid Transfer Protein ◽  
Specific Ige ◽  
Alcoholic Beverages ◽  
Food Groups ◽  
Lipid Transfer ◽  
Case Presentation ◽  
Blue Cheese ◽  
Transfer Protein ◽  
Ige Reactivity ◽  
Specific Proteins

Background. Allergy to beer is often due to specific proteins in barley and sometimes to lipid transfer protein. Allergy to wine is frequently due to a sensitivity to grape proteins. We present a rare case of allergy to beer, wine, and cider resulting from IgE reactivity to yeasts and moulds which also explained the patient’s additional sensitivity to yeast extracts and blue cheese. Case Presentation. The patient’s symptoms included throat and facial itching accompanied by mild wheeze and severe urticaria. Diagnosis of allergy to yeast was confirmed by specific IgE testing as well as that to relevant foods and beverages. The patient’s ongoing management included advice to avoid beer, wine, and other food groups containing specific yeasts, in addition to carrying a short acting nonsedating antihistamine as well as an adrenaline autoinjector. Conclusions. Cases of yeast allergy are extremely rare in medical literature but may be underrecognised and should be considered in patients presenting with reactions to alcoholic beverages and other yeast-containing products.

scholarly journals Risk Factors in Severe Anaphylaxis: Which Matters The Most, Food or Cofactors?

2021 ◽  
Vol 32 (4) ◽  
Author(s):  
R Casas-Saucedo ◽  
C de la Cruz ◽  
G Araujo-Sánchez ◽  
S Gelis ◽  
T Jimenez ◽  
...  
Keyword(s):  
Risk Factors ◽  
Anaphylactic Shock ◽  
Lipid Transfer Protein ◽  
Clinical History ◽  
Food Group ◽  
Plant Food ◽  
Food Groups ◽  
Lipid Transfer ◽  
Related Factors ◽  
Prick Test

Background: The prevalence of anaphylactic shock, the most severe manifestation of anaphylaxis, remains unknown. Risk factors and biomarkers have not been fully identified. Objective: To identify risk factors in anaphylactic shock patients. Methods: Using lipid transfer protein (LTP)allergy as a model, the characteristics of patients who developed anaphylaxis and anaphylactic shock were compared. Demographics, pollen sensitization, foods ingested up to 2 hours before the reaction onset, and the presence of a cofactor were recorded. Culprit foods were identified by compatible clinical history and positive allergological work-up (skin prick test and/or sIgE). Results:150 reactions were evaluated, suffered by 55 patients with An (134 reactions) and 12 with AnS (16 reactions). Patients in the anaphylaxis group experienced twice as many reactions (mean [SD] 2.4[2.5] in An vs 1.3[1.5) in AnS, p<0.02). No relationship between any food group and reaction severity was found. The most frequent food involved in both groups of patients was the combination of several plant-derived foods (“plant food mix”), followed by peach and nuts. Indeed, in the “plant food mix” reactions the presence of a cofactor was more often observed than in other food groups. On the other hand, cofactors were not present in peach- and nuts-related reactions. Exercise was the most frequent cofactor in all groups. Conclusion: In our series, the severity of the reactions was not determined by the kind of food or presence of a cofactor. Anaphylactic shock seems an infrequent presentation that may be related with other individual-related factors that need further evaluation.

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