In vitro Evaluation of Skin Sensitivity of Povidone-Iodine and Other Antiseptics Using a Three-Dimensional Human Skin Model

Dermatology ◽  
2002 ◽  
Vol 204 (1) ◽  
pp. 109-113 ◽  
Author(s):  
Mieko Nagasawa ◽  
Hiroyuki Hayashi ◽  
Takemi Nakayoshi
Keyword(s):  
Human Skin ◽  
Povidone Iodine ◽  
Three Dimensional ◽  
Skin Model ◽  
Skin Sensitivity ◽  
In Vitro Evaluation

Cosmetic efficacy claims in vitro using a three-dimensional human skin model

2001 ◽  
Vol 23 (5) ◽  
pp. 309-318 ◽  
Author(s):  
K. Schlotmann ◽  
M. Kaeten ◽  
A. F. Black ◽  
O. Damour ◽  
M. Waldmann-Laue ◽  
...  
Keyword(s):  
Human Skin ◽  
Three Dimensional ◽  
Skin Model

Assessment of the Ocular Irritancy Potential of Hair Conditioners in a Three-Dimensional in Vitro Human Skin Model

1994 ◽  
Vol 13 (2) ◽  
pp. 117-125 ◽  
Author(s):  
T. Donnelly ◽  
B. Decker ◽  
M. Stemp ◽  
L. A. Rheins ◽  
P. Logemann
Keyword(s):  
Human Skin ◽  
Three Dimensional ◽  
Skin Model

scholarly journals Development and Evaluation of a Topical Anti-Inflammatory Preparation Containing Dodonaea polyandra Extract

2015 ◽  
Vol 18 (4) ◽  
pp. 578 ◽  
Author(s):  
Bradley S Simpson ◽  
Xianling Luo ◽  
Jiping Wang ◽  
Yunmei Song ◽  
David Claudie ◽  
...  
Keyword(s):  
Phase Separation ◽  
Human Skin ◽  
Dosage Form ◽  
Interleukin 1 ◽  
In Vitro Release ◽  
Skin Inflammation ◽  
Skin Model ◽  
Inflammation Model ◽  
Anti Inflammatory

Purpose: We have previously reported that the Australian Northern Kaanju (Kuuku I’yu) medicinal plant Dodonaea polyandra has anti-inflammatory activity. This is attributed largely to the presence of clerodane diterpenoids contained within the leaf resin. We envisaged developing a topical preparation to treat indications relating to skin inflammation. However, it was unknown whether the resin could be incorporated into a suitable dosage form while retaining the therapeutic value demonstrated in previous work. Therefore, the following study was undertaken to assess parameters of safety and efficacy for a prototype formulation containing the leaf resin extracted from D. polyandra. Methods: Using the assessment criteria of optimum appearance, tactile feeling, spreadability and odour, 78 different formulations were developed. Formulation stability was assessed using a centrifugal test with preparations displaying phase separation further modified or re-formulated. A prototype formulation containing 5% w/w plant resin was selected and subjected to in vitro release studies. This was quantified through HPLC analysis using two major bioactive diterpenoids as reference. The prototype formulation was tested for efficacy in a TPA-induced acute murine skin inflammation model as well as a 3D human skin model for irritancy/toxicity (Epiderm™). Results: The prototype resin cream was a chartreuse-coloured homogenous semisolid preparation that was readily spreadable upon contact with skin with no sensation of tackiness, residual greasiness, or irritation. The optimized cream showed no phase separation after 30 min centrifugation at 825 g. In the TPA-induced inflammation model, the resin formulation significantly reduced ear thickness and interleukin-1 beta levels in mouse ear tissue. The 5% w/w resin cream formulation showed no irritancy in a 3D human skin model. Conclusions: Our results demonstrate that bioactive resin from D. polyandra can be formulated into a stable and non-irritant semi-solid dosage form and reduce parameters of acute skin inflammation in vivo. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

Skin permeation of retinol in Tween 20-based deformable liposomes: in-vitro evaluation in human skin and keratinocyte models

2006 ◽  
Vol 58 (2) ◽  
pp. 161-166 ◽  
Author(s):  
Yu-Kyoung Oh ◽  
Mi Young Kim ◽  
Jee-Young Shin ◽  
Tae Woon Kim ◽  
Mi-Ok Yun ◽  
...  
Keyword(s):  
Human Skin ◽  
Skin Permeation ◽  
Tween 20 ◽  
In Vitro Evaluation

scholarly journals Construction of Three-dimensional Human Skin Model Involving Dendritic Cells and Its Application to Skin Sensitization Test

2008 ◽  
Vol 128 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Tadashi UCHINO ◽  
Toshiaki TAKEZAWA ◽  
Yoshiaki IKARASHI ◽  
Hiroshi TOKUNAGA
Keyword(s):  
Dendritic Cells ◽  
Human Skin ◽  
Three Dimensional ◽  
Skin Sensitization ◽  
Skin Model

scholarly journals UVA and UVB formulation phototoxicity in a three-dimensional human skin model: Photodegradation effect

2018 ◽  
Vol 53 ◽  
pp. 37-44 ◽  
Author(s):  
Dayane P. uco ◽  
Vânia R. Leite-Silva ◽  
Heron D.T. Silva ◽  
Marcelo D. Duque ◽  
Jeffrey Grice ◽  
...  
Keyword(s):  
Human Skin ◽  
Three Dimensional ◽  
Skin Model

scholarly journals Inhibitory Effects of α-Arbutin on Melanin Synthesis in Cultured Human Melanoma Cells and a Three-Dimensional Human Skin Model

2004 ◽  
Vol 27 (4) ◽  
pp. 510-514 ◽  
Author(s):  
Kazuhisa Sugimoto ◽  
Takahisa Nishimura ◽  
Koji Nomura ◽  
Kenji Sugimoto ◽  
Takashi Kuriki
Keyword(s):  
Human Skin ◽  
Three Dimensional ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Melanin Synthesis ◽  
Inhibitory Effects ◽  
Skin Model ◽  
Human Melanoma Cells

scholarly journals Preliminary Study of In Vitro Three-Dimensional Skin Model Using an Ovine Collagen Type I Sponge Seeded with Co-Culture Skin Cells: Submerged versus Air-Liquid Interface Conditions

Polymers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 2784
Author(s):  
Mh Busra Fauzi ◽  
Zahra Rashidbenam ◽  
Aminuddin Bin Saim ◽  
Ruszymah Binti Hj Idrus
Keyword(s):  
Collagen Type ◽  
Three Dimensional ◽  
Collagen Type I ◽  
Liquid Interface ◽  
Type I ◽  
Skin Model ◽  
Skin Cells ◽  
Air Liquid Interface ◽  
In Vitro Skin Model

Three-dimensional (3D) in vitro skin models have been widely used for cosmeceutical and pharmaceutical applications aiming to reduce animal use in experiment. This study investigate capability of ovine tendon collagen type I (OTC-I) sponge suitable platform for a 3D in vitro skin model using co-cultured skin cells (CC) containing human epidermal keratinocytes (HEK) and human dermal fibroblasts (HDF) under submerged (SM) and air-liquid interface (ALI) conditions. Briefly, the extracted OTC-I was freeze-dried and crosslinked with genipin (OTC-I_GNP) and carbodiimide (OTC-I_EDC). The gross appearance, physico-chemical characteristics, biocompatibility and growth profile of seeded skin cells were assessed. The light brown and white appearance for the OTC-I_GNP scaffold and other groups were observed, respectively. The OTC-I_GNP scaffold demonstrated the highest swelling ratio (~1885%) and water uptake (94.96 ± 0.14%). The Fourier transformation infrared demonstrated amide A, B and I, II and III which represent collagen type I. The microstructure of all fabricated sponges presented a similar surface roughness with the presence of visible collagen fibers and a heterogenous porous structure. The OTC-I_EDC scaffold was more toxic and showed the lowest cell attachment and proliferation as compared to other groups. The micrographic evaluation revealed that CC potentially formed the epidermal- and dermal-like layers in both SM and ALI that prominently observed with OTC-I_GNP compared to others. In conclusion, these results suggest that OTC_GNP could be used as a 3D in vitro skin model under ALI microenvironment.

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